Bone regenerative medicine by introducing ets family of gene

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Ets oncogene family plays an important role for basic biological events such as embryogenesis, differentiation, cell growth and apoptosis. Previously, we found that one of the ets family, E1AF, activates the expression of matrixmetalloproteases (MMPs), which is related to bone production. The objective of this research is to examine whether ets genes induce the differentiation of osteoblast.We produced an E1AF expression vector and examined the activation of osteopontin gene expression by luciferase assay. The expression was activated by E1AF in dose-dependent manner.Since E1AF protein failed to be detected in the cells expressing E1AF stably, whereas the mRNA was expressed well, E1AF is regulated in post-transcriptional level. We found that E1AF is degraded by ubiquitin-proteasome pathway.We examined the effect of adenovirus E1A, which inhibits bone production, on the activation of gene expression mediated by E1AF. The data showed that E1A inhibited the transcription from the gene targeted by E1AF.These findings indicate that E1AF has some relation with bone production.