Response to synovial fibroblasts by various stresses

滑膜成纤维细胞对各种应激的反应

• 批准号: 15592123
• 负责人: KAWAKAMI Tetsuji
• 金额: $ 2.18万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592123/
• 关键词: Temporomandibular joint(TMJ); Cyclooxygenase-2(COX-2); Mechanical strech; Synovial fibroblasts; Nuclear factor-κB(NF-κB); 顎関節症; Cyclppxygenase-2(COX-2); 滑膜細胞; Nuclear factor-κB(NF-κB); Cyclooxygenase-2(COX-2)

It is proposed that synovial inflammation mediated by induced cyclooxygenase-2(COX-2) have important roles in the onset of temporomandibular joint(TMJ) pain. However, we have little knowledge about the pathophysiology of TMJ pain. In this study, the effect of uni-axial mechanical stretch on the expression of COX-2 was investigated in lapine synovial fibroblast cell line (HIG-82). Western blot analyses indicated that the level of the expression of COX-2 protein in HIG-82 increased in response to mechanical stretch (140%). We also observed nuclear translocation of p65 which is one of the nuclear factor-κB(NF-κB) family members in response to stretching. To investigate whether the induction of COX-2 was under NF-κB control was analyzed by luciferase reporter assay. Compared with the control, mechanical stretch treatment significantly increased the promoter activity of the NF-κB. These results showed that mechanical stress on synovial membrane induced COX-2 via activation of NF-κB. The present study suggested that synovial inflammation due to repetitive motion, such as bruxism might lead to TMJ pain.

提示诱导型环氧合酶-2（考克斯-2）介导的滑膜炎症在颞下颌关节（TMJ）疼痛的发生中起重要作用。然而，我们对颞下颌关节疼痛的病理生理学知之甚少。本研究以兔滑膜成纤维细胞（HIG-82）为研究对象，探讨了单向机械牵张对考克斯-2表达的影响。Western blot分析表明，考克斯-2蛋白在HIG-82中的表达水平响应于机械拉伸而增加（140%）。我们还观察到牵拉引起的核因子-κB（NF-κB）家族成员p65的核转位。采用荧光素酶报告基因分析法研究考克斯-2的诱导是否受NF-κB的调控。与对照组相比，机械牵张处理显著提高了NF-κB的启动子活性。以上结果表明，机械应力作用于滑膜后，通过激活NF-κB，诱导考克斯-2的表达。目前的研究表明，由于重复运动，如磨牙症，滑膜炎症可能会导致颞下颌关节疼痛。

项目成果

Induction of COX-2 in lapine Synovial fibroblasts by uni-axial mechanical strech

单轴机械拉伸在兔滑膜成纤维细胞中诱导 COX-2

• 发表时间: 2003
• 期刊: J.Jpn.Stomatol.Soc. 52(5)
• 作者: Tetsuji Kawakami;Toshiaki Morisugi;Naoyuki Oheda;Hirohito Fujita;Yasuro Yoshimura;Tadaaki Kirita
• 通讯作者: Tadaaki Kirita

川上哲司他: "伸展ストレスによるウサギ滑膜細包のCOX-2発現誘導"日本口腔科学会雑誌. 52巻5号. 235-242 (2003)

Tetsushi Kawakami 等人：“拉伸应激诱导兔滑膜滤泡中的 COX-2 表达”，日本口腔医学会杂志，第 52 卷，第 5 期，第 235-242 期（2003 年）。

伸展ストレスによるウサギ滑膜細胞のCOX-2発現誘導

拉伸应力诱导兔滑膜细胞中COX-2表达

• 发表时间: 2003
• 期刊: 日本口腔科学会雑誌 52巻5号
• 作者: 川上哲司;森杉敏明;桐田忠昭
• 通讯作者: 桐田忠昭