The effect of the temporomandibular synovial cells bythe mechanical stretch

机械拉伸对颞下颌关节滑膜细胞的影响

• 批准号: 22592227
• 负责人: KAWAKAMI Tetsuji
• 金额: $ 2.66万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592227/
• 关键词: 滑膜細胞; 顎関節; 酸化ストレス; 遺伝子発現

Temporomandibular disorders (TMD) show complex symptoms associated with inflammation, pain and degeneration of the peripheral tissues including synovium. Although it is believed that excessive mechanical stress on synovium causes development of TMD, the molecular mechanism by which mechanical stress triggers TMD has still remained unclear. In order to examine the effect of mechanical stress on synoviocytes, rabbit synovial cells were cyclically stretched in vitro. The stretch efficiently increased the gene expressions of cyclooxygenase-2, inducible nitric oxide synthase and NF-κB responsive reporter gene constructs. The results demonstrate that mechanical stress on synovial cells not only induces gene expressions of COX-2 and iNOS but also affects PAR synthesis.

颞下颌关节紊乱病（Temporomandibular disorders，TMD）是一种以炎症、疼痛和滑膜等周围组织变性为主要表现的复杂疾病。尽管人们认为过度的机械应力作用于滑膜是导致TMD发生的原因，但机械应力触发TMD的分子机制仍不清楚。为了研究机械应力对滑膜细胞的影响，在体外循环拉伸兔滑膜细胞。拉伸有效地增加了环氧合酶-2、诱导型一氧化氮合酶和NF-κB应答报告基因构建体的基因表达。结果表明，机械应力不仅诱导滑膜细胞考克斯-2和iNOS的基因表达，而且影响PAR的合成。

项目成果

Mechanical stretch enhance NF- κB-dependent gene expression and poly (ADP-ribose) synthesis in synovial cells

机械拉伸增强滑膜细胞中 NF-κB 依赖性基因表达和聚 (ADP-核糖) 合成

• 发表时间: 2010
• 期刊: The Journal of Biochemistry
• 作者: Morisugi T;Tanaka Y;Kawakami;Kirita T
• 通讯作者: Kirita T

滑膜線維芽細胞における15デオキシプロスタグランジンで誘導されるPPARγ非依存的アポトーシス

15-脱氧前列腺素诱导的滑膜成纤维细胞中 PPARγ 独立的细胞凋亡

• 发表时间: 2011
• 作者: Morisugi T;Tanaka Y;Kawakami;Kirita T;田中康春
• 通讯作者: 田中康春