Regulated factors of NF-kB activity in synovial fibroblasts by repetitive mechanical stretch

提示诱导型环氧合酶-2（考克斯-2）介导的滑膜炎症在颞下颌关节（TMJ）疼痛的发生中起重要作用。然而，我们对颞下颌关节疼痛的病理生理学知之甚少。在我们以前的研究中，我们已经证明，考克斯-2和转录因子核因子κB（NF-κB）诱导兔滑膜成纤维细胞（HIG-82）过度机械拉伸（140%峰到峰）。在这项研究中，我们研究了考克斯-2和NF-κB B是否在HIG-82细胞中被重复和弱拉伸（120%峰-峰）诱导，如磨牙症和紧咬。此外，我们还检测了机械牵张诱导HIG-82细胞NF-κB活性的调控因素，报告基因检测结果显示，随着牵张时间的增加，HIG-82细胞NF-κB活性短暂升高，随后逐渐降低，提示动态弱重复牵张组NF-κ B活性显著高于静态过度牵张组，且随着牵张时间的延长，NF-κB活性逐渐降低。 ...更多信息 恶心。同样，考克斯-2蛋白在重复机械应力诱导下也短暂增加。为了检测机械牵张诱导的HIG-82细胞NF-κB活性的调节因子，将细胞与考克斯-2抑制剂孵育1小时在循环拉伸之前，将样品（BAY 11 -7085（2μM）、姜黄素（10 μ M-40μM）、MG 132（2μM）、PDTC（5μM）和SP 600125（5μM））进行离心。考克斯-2抑制剂（姜黄素、BAY 11 -7085、MG 132和PDTC）可显著抑制拉伸引起的NF-κB活性。同样，考克斯-2抑制剂抑制考克斯-2的诱导响应重复拉伸。其中，姜黄素是食品香料姜黄的主要成分，以抗炎和抗氧化特性而闻名。提示考克斯-2抑制剂（如姜黄素）可作为治疗TMD的一种新方法。本研究提示，磨牙、紧咬等重复性运动引起的滑膜炎症可能导致TMJ疼痛，而预先给予考克斯-2抑制剂可抑制滑膜细胞的炎症。少

It is proposed that synovial inflammation mediated by induced cyclooxygenase-2 (COX-2) has important roles in the onset of temporomandibular joint (TMJ) pain. However, we have little knowledge about the pathophysiology of TMJ pain. In our previous studies, we have demonstrated that COX-2 and the transcription factor nuclear factor κB (NF-κB) were induced in lapine synovial fibroblast cells (HIG-82) by excessive mechanical stretch (140% peak to peak). In this study, we examined whether COX-2 and NF-κB were induced in HIG-82 cells by repetitive and weak stretch (120% peak to peak) such as bruxism and clenching. Moreover, we examined regulated factors of NF-κB activity induced by mechanical stretch in HIG-82 cells.Reporter gene assay showed that NF-κB activity in HIG-82 transiently increased according to the increase of stretching periods and decreased gradually, suggesting that NF-κB activity in HIG-82 with dynamic weak repetitive stretch was significantly higher than static excessive st … More retch. Similarly, COX-2 protein induced by repetitive mechanical stress transiently increased. This suggested that synovial inflammation due to repetitive motion might lead to TMJ pain.To examine regulated factors of NF-κB activity induced by mechanical stretch in HIG-82 cells, the cells were incubated for 1 hour with some COX-2 inhibitors (BAY11-7085 (2μM), curcumin (10〜40μM), MG132 (2μM), PDTC (5μM), and SP600125 (5μM) ) prior to cycling stretch. NF-κB activity in response to stretching is significantly suppressed by COX-2 inhibitor (curcumin, BAY11-7085, MG132 and PDTC). Similarly, COX-2 inhibitors suppressed induction of COX-2 in response to repetitive stretch. Among them, curcumin, a major component of food spice turmeric, is known for anti-inflammatory and antioxidant properties. This suggested that pharmacotherapy using COX-2 inhibitors (e.g. curcumin) could be applied to a new treatment for TMD.The present study suggested that synovial inflammation due to repetitive motion, such as bruxism and clenching, might lead to TMJ pain, and that inflammation of synoviocyte was able to suppress by pretreatment of COX-2 inhibitors. Less