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Assessment of Children's Psychological Stress using Salivary Amylase Activity

利用唾液淀粉酶活性评估儿童心理压力

基本信息

批准号：
15592160
负责人：
SANO Tomiko
金额：
$ 2.3万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592160/
关键词：
Salivary Amylase Activity Pediatric Dentistry Dental Treatment Stress Dental Fear

项目摘要

The aim of the present study was to assess the psychological stress of children using salivary amylase activity. The subjects consisted of 13 patients who attended the Pediatric Dental Clinic of Niigata University Medical and Dental Hospital for periodical examinations. They ranged from 8 to 11 years of age (The mean age being 10.7). The subjects who received extraction of teeth, cavity preparation, and application of fluoride were excluded. The first saliva samples were obtained when the child arrived for the dental visits. The second saliva samples were obtained after treatment immediately, and the third saliva samples were obtained 15 minutes after treatment.Control subjects were 9 dentists who were in good health. The average age of the control subjects was 33.4 yr (ranging 26-42 yr). Saliva samples were also obtained in the same manner. These samples had been kept at -20℃ until the hormone measurements were done.In the child patients, salivary amylase activity of the third saliva samples was significantly lower (p<0.05) than that of the first saliva samples. However in the control subjects, there were no significant differences between the salivary amylase activities obtained before and after treatment.The results of the present study indicated that children felt anxiety when they arrived for the dental visits, but children's stress was allayed within 15 minutes after treatments. As a method of measuring stress in children, measurement of salivary amylase activity may be recommended. This is the first report that we assess the psychological stress in children using salivary amylase activity in pediatric dentistry.

本研究的目的是利用唾液淀粉酶活性评估儿童的心理压力。研究对象为在新泻大学医学齿科医院儿童牙科诊所定期检查的13名患者。他们的年龄从 8 岁到 11 岁不等（平均年龄为 10.7 岁）。接受拔牙、蛀牙准备和氟化物应用的受试者被排除在外。第一份唾液样本是在孩子到达牙科诊所时采集的。第二份唾液样本是在治疗后立即获取的，第三份唾液样本是在治疗后15分钟获取的。对照对象是9名身体状况良好的牙医。对照组的平均年龄为 33.4 岁（范围为 26-42 岁）。也以相同的方式获取唾液样本。这些样本在进行激素测量之前一直保存在-20℃下。在儿童患者中，第三个唾液样本的唾液淀粉酶活性显着低于第一个唾液样本的唾液淀粉酶活性（p<0.05）。然而，在对照组中，治疗前后获得的唾液淀粉酶活性没有显着差异。本研究结果表明，儿童在去看牙医时感到焦虑，但儿童的压力在治疗后15分钟内得到缓解。作为测量儿童压力的方法，可能建议测量唾液淀粉酶活性。这是我们在儿科牙科中使用唾液淀粉酶活性评估儿童心理压力的第一份报告。