The influence of bone formation by thrombomodulin in platelet rich plasma gel
富血小板血浆凝胶中血栓调节蛋白对骨形成的影响
基本信息
- 批准号:15592193
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Platelet-rich plasma (PRP) has been shown to enhance the maturation of bone grafts following local application, and to have biological effects on osteoblasts in vitro. However, PRP is not applied by itself clinically due its poor benefits in large bone defects. The present study was undertaken to develop a clinical alternative to autologous bone, by investigating the application of PRP in combination with osteoblastic cells and evaluating its effects after transplantation.PRP and platelet-poor plasma (PPP) were prepared from blood obtained from ddY mice by two centrifugation steps. MC3T3-E1 cells were labeled with fluorescent carbocyanine just before transplantation. The combination of labeled cells and PRP gel was subcutaneously transplanted into the back of SCID mice, and the transplants were evaluated radiographically and immunohistologically after 4 weeks. The effects of PRP were assessed by alkaline phosphatase (ALP) staining and von Kossa staining and the expressions of bone-related markers were analyzed by reverse transcription-polymerase chain reaction before transplantation.Before transplantation, PRP enhanced the expressions of Osterix and bone sialoprotein mRNAs compared with PPP. Furthermore, PRP elevated ALP activity and induced the formation of mineralized nodules. After transplantation, the combination of labeled cells and PRP gel formed mineralized tissue and the transplanted cells visualized in the tissue using fluorescence microscopy expressed osteocalcin and type I collagen. And Phosphorylation of focal adhesion molecule (FAK) was recognized in osteoblasts with PRP application.These results suggest that application of a PRP/osteoblasts complex has beneficial effects for transplanting engineered cells into bone defects through the promotion of osteoblastic differentiation.
富血小板血浆(PRP)在局部应用后可促进骨移植物的成熟,并在体外对成骨细胞具有生物学效应。然而,由于PRP在大面积骨缺损中的益处较差,因此临床上不单独应用PRP。本研究旨在通过研究PRP与成骨细胞联合应用并评估其移植后的效果来开发一种临床上可替代自体骨的方法。在移植前用荧光碳菁标记MC 3 T3-E1细胞。将标记细胞与PRP凝胶组合物皮下移植到SCID小鼠背部,4周后对移植物进行放射学和免疫组织学评价。移植前采用碱性磷酸酶(ALP)染色和von Kossa染色评价PRP的作用,并采用逆转录-聚合酶链反应(RT-PCR)检测骨相关标志物的表达,结果表明:与PPP相比,PRP可促进骨相关标志物Osterix和骨唾液酸蛋白mRNA的表达。此外,PRP升高ALP活性并诱导矿化结节的形成。移植后,标记的细胞和PRP凝胶的组合形成矿化组织,并且使用荧光显微镜在组织中可见的移植细胞表达骨钙素和I型胶原。结果表明,PRP/成骨细胞复合物可促进成骨细胞的分化,有利于工程化细胞的骨缺损修复。
项目成果
期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Platelet-Rich Plasma/Osteoblasts complex Induces Bone Formation via Ostoblastic Differentiation Following Subcutaneous Transplantation
富含血小板的血浆/成骨细胞复合物通过皮下移植后的成骨细胞分化诱导骨形成
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Goto H;Matsuyama T;Miyamoto M;Yonamine Y;Izumi Y.
- 通讯作者:Izumi Y.
Platelet-rich plasma/osteoblasts complex induces bone formation via osteoblastic differentiation following subcutaneous transplantation
- DOI:10.1111/j.1600-0765.2006.00892.x
- 发表时间:2006-10-01
- 期刊:
- 影响因子:3.5
- 作者:Goto, H.;Matsuyama, T.;Izumi, Y.
- 通讯作者:Izumi, Y.
Expression of major histocompatibility complex class II and CD80 by gingival epithelial cells induces activation of CD4+ T cells in response to bacterial challenge
- DOI:10.1128/iai.73.2.1044-1051.2005
- 发表时间:2005-02-01
- 期刊:
- 影响因子:3.1
- 作者:Matsuyama, T;Kawai, T;Taubman, MA
- 通讯作者:Taubman, MA
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MATSUYAMA Takashi其他文献
MATSUYAMA Takashi的其他文献
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{{ truncateString('MATSUYAMA Takashi', 18)}}的其他基金
Elucidation of the molecular mechanism for highly active terminator regions in Saccharomyces cerevisiae
阐明酿酒酵母高活性终止子区域的分子机制
- 批准号:
25660071 - 财政年份:2013
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Establishment of Peri-implantitis treatment using a dual-purpose graft and cell transplantation.
建立使用双重目的移植物和细胞移植的种植体周围炎治疗方法。
- 批准号:
21592629 - 财政年份:2009
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Elucidation of new adhesion mechanism between neutrophils and gingival epithelial cells via thrombomodulin
通过血栓调节蛋白阐明中性粒细胞与牙龈上皮细胞之间的新粘附机制
- 批准号:
13672193 - 财政年份:2001
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Real-Tune 3D Shape Reconstruction, Visualization, Editing, and Coding for 3D Video
实时调整 3D 形状重建、可视化、编辑和 3D 视频编码
- 批准号:
13308017 - 财政年份:2001
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Understanding human intention and activities for versatile real-time human-machine-interactions
了解人类意图和活动以实现多功能实时人机交互
- 批准号:
13224051 - 财政年份:2001
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Cooperative Distributed Image Understanding Systems
协作分布式图像理解系统
- 批准号:
08408010 - 财政年份:1996
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Developing High Precision Versatile Camera Systems by Multi-Image Integration
通过多图像集成开发高精度多功能相机系统
- 批准号:
07558047 - 财政年份:1995
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Parallel Image Understanding System on Recursive Torus Architecture
递归环面架构的并行图像理解系统
- 批准号:
05452359 - 财政年份:1993
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Geometric Programming System
几何规划系统
- 批准号:
03452171 - 财政年份:1991
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Effects of Doxorubicin or OK-432 on Cancer cells during liver regeneration after two-thirds hepatectomy
阿霉素或 OK-432 对三分之二肝切除术后肝再生过程中癌细胞的影响
- 批准号:
03670630 - 财政年份:1991
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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