Mapping of Nuclear Matrix Binding Region of Human Genome and Functional Analysis of A Nuclear Matrix Protein

我们研究了大鼠核支架蛋白P130的分子基础及其功能。由845个氨基酸残基组成的P130具有几个功能结构域，通过改变其磷酸化状态，改变其磷酸化状态，使其从细胞核的洋地黄素可提取部分转移到核支架，从而产生一种电泳学上独特的异构体P123。功能结构域NLS、NES和含锌指DNA结合域ZF1和ZF2有助于这些易位。P130通过与hnRNPs I和L相似的两个RNA结合域(RB1和RB2)与RNA结合，在大鼠肝脏和Ac2F肝癌细胞中发现微体和多聚体定位的P130和P123。这种定位需要P130事先进入细胞核，但不需要RB1和RB2。因此，最初从大鼠肝核支架中纯化的P130不仅在核支架中，而且在各种亚细胞室中都有可能在多种生物学事件中发挥作用。P130(AB205483)与Matrin 3(M63485和BC062231)相同，但自首次发表以来，对大鼠Matrin 3的一级结构进行了修改。2000；http：//gdb www.gdb.org/)用于MAR/SAR搜索程序[MARFinder或SMARTER(Friscm等人)。因此，我们已经定位了人类基因组中30个基因的100多个MARS/http://www.genomatix.de/cgi-bin/smartest。

We examined the molecular basis of rat P130, a nuclear scaffold protein, and its functions. P130 comprising 845 amino acid residues possesses several functional domains and yields an electrophoretically distinctive isoform, P123, by altering its phosphorylation status in association with translocation across the nuclear membrane and from the digitonin-extractable fraction of the nucleus to the nuclear scaffold. The functional domains, NLS, NES, and zinc-finger bearing DNA-binding domains, ZF1 and ZF2, aid these translocations. P130 binds RNA through two RNA-binding domains (RB1 and RB2) similar to those of hnRNPs I and L.Microsome- and polysome-localized P130 and P123 were found in rat liver and Ac2F hepatoma cells. This localization required prior entry of P130 to the nucleus, but did not require RB1 and RB2. Thus, P130 initially purified from rat liver nuclear scaffold has the potential to play a variety of roles in biological events not only in the nuclear scaffold but also in various subcellular compartments. P130 (AB205483) is identical to matrin 3 (M63485 and BC062231), although the primary structure of rat matrin 3 has been revised, since it was first published.The Human Genome Database (GDB) (Future et al. 2000 ; http::/gdbwww.gdb.org/) was used for MAR/SAR search program [MARFinder or SMARTest (Friscm et al. 2002 ; http://www.genomatix.de/cgi-bin/smartest pd/smartest.pl)] As a consequence, we have mapped more than 100 MARs/SARs of 30 genes in human genome.