Effect of fetal exposure to multiple chemicals on the epigenetic regulation in the testis of mice

胎儿接触多种化学物质对小鼠睾丸表观遗传调控的影响

• 批准号: 17590148
• 负责人: MATSUNO Yoshiharu
• 金额: $ 2.3万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590148/
• 关键词: multiple chemical; exposure; epigenetic; testis; Restriction Landmark Genomics Scanning (RLGS); cleft palate (CP); レーザーマイクロダイセクション; 精巣上体; 子宮

The main purpose of the study was to examine the effect of fetal exposure to multiple chemical (Diethlstilbestrol) on epigenetic alteration in mouse testis. However, we did not obtain a clear finding using Restriction Landmark Genomics Scanning (RLGS) method for male reproductive tract. On the other hand, cleft palate (CP) in fetuses were manifested by maternal exposure to all-trans retinoic acid (atRA) that was the congenital anomaly, and was able to obtain a few findings concerning the generation mechanism of epigenetic with the chemical.CP is caused by both genetic and environmental factors. Although numerous genetic studies have been performed in the past, with various candidate genes suggested, the etiology remains yet unclear. However, in recent years, epigenetics that participates in the development and the differentiation of cells has received the spotlight as a new approach. In this report, an epigenetic approach (in specific, DNA methylation) was utilized in order to analyze … More the mechanisms of CP.CPs in fetuses were manifested by maternal exposure to atRA. Their secondary palates were excised for analysis. The cytosine extension assay (CEA) and RLGS were performed to analyze DNA methylation status. The expression levels of the DNA methyltransferases were examined by real- time RT-PCR.Using CEA, on gestation day (GD) 14.5, the status of DNA methylation within CpG islands and in global DNA was significantly decreased in atRA-treated groups compared to the controls (p<0.01 and p<0.05). In the controls, the status within CpG islands on GD14.5 was significantly increased compared to GD13.5 and 18.5, p<0.01. Using real-time RT-PCR, there was no significant change in the expression of Dnmts, except for on GD 18.5. Using RLGS on GD 18.5, 5 spots (0.49%) in the controls and 1 spot (0.1%) in atRA-treated groups were specifically detected.These results indicate that changes in DNA methylation may play an important role in the manifestation of CP caused by environmental factors such as maternal exposure to atRA. Less

该研究的主要目的是检查胎儿暴露于多种化学物质（Diethlstilbestrol）对小鼠测试中表观遗传改变的影响。但是，我们没有使用限制性地标基因组学扫描（RLGS）方法来获得明确的发现，用于男性生殖道。另一方面，胎儿中的left裂（CP）表现出孕产妇暴露于先天性异常的全反式视黄酸（ATRA），并且能够获得一些有关表观遗传学的生成机制的发现。CP引起的遗传和环境因素引起了许多遗传学的研究。不清楚。然而，近年来，参与发育和细胞的分化的表观遗传学已成为一种新方法。在本报告中，利用了一种表观遗传学方法（特定的DNA甲基化）来分析……更多的CP.CPS在胎儿中的机制通过母体暴露于Atra来表现出胎儿的机制。他们的次要味觉非常适合分析。进行了胞嘧啶扩展测定（CEA）和RLGS以分析DNA甲基化状态。通过实时RT-PCR进行CEA，在妊娠日（GD）14.5通过实时RT-PCR进行了DNA甲基转移酶的表达水平，与对照组相比，ATRA处理组中CPG岛内DNA甲基化的状态和全球DNA的状态显着降低（P <0.01和P <0.01和P <0.05）。在对照组中，与GD13.5和18.5，p <0.01相比，GD14.5上CpG岛上的状态显着增加。使用实时RT-PCR，除GD 18.5外，DNMT的表达没有显着变化。使用在GD 18.5上的RLG，在对照组中进行了5个斑点（0.49％），在ATRA处理组中有1个斑点（0.1％）。这些结果表明，DNA甲基化的变化可能在由环境因素（例如MATH暴露于ATRA）引起的CP表现中起重要作用。较少的

项目成果

Formaldehyde exposure in a gross anatomy laboratory - personal exposure level in higher than indoor concentration.

大体解剖实验室中的甲醛暴露 - 个人暴露水平高于室内浓度。

• 发表时间: 2006
• 期刊: Environmental science and pollution research international 13
• 作者: Ohmichi K;Komiyama M;Matsuno Y;Takahashi Y;Miyamoto H;Kadota T;Maekawa M;Toyama Y;Tatsugi Y;Kohno T;Ohmichi M;Mori C.
• 通讯作者: Mori C.

DNA methylation changes in the secondary palate of fetuses with cleft palate in maternal mice exposed to all-trans retinoic acid

暴露于全反式视黄酸的母鼠腭裂胎儿次级腭 DNA 甲基化变化

• 期刊: (Submitted)
• 作者: M.Kuriyama;A.Udagawa;S.Yoshimoto;M.Ichinose;K.Sato;K.Yamazaki;Y.Matsuno;K.Shiota;C.Mori
• 通讯作者: C.Mori

Relationship between exposure to formaldehyde and immunoglobulin E (IgE) production during the gross anatomy laboratory.

大体解剖实验室期间甲醛暴露与免疫球蛋白 E (IgE) 产生之间的关系。

• 发表时间: 2006
• 期刊: Journal of Health Science 52
• 作者: Ohmichi K;Komiyama M;Matsuno Y;Y Sawabe;Miyaso H;Fukata H;Ohmichi M;Kadota T;Nomura F;Mori C
• 通讯作者: Mori C

Formaldehyde exposure in a gross anatomy laboratory -personal exposure level is higher than indoor concentration.

大体解剖实验室中的甲醛暴露-个人暴露水平高于室内浓度。

• 发表时间: 2006
• 期刊: Environmental science and pollution research international 13
• 作者: Ohmichi K;Komiyama M;Matsuno Y;Takahashi Y;Miyamoto H;Kadota T;Maekawa M;Toyama Y;Tatsugi Y;Kohno T;Ohmichi M;Mori C
• 通讯作者: Mori C

DNA methylation changes in the secondary palate of fetuses with cleft palate in maternal mice exposed to all-trans Retinoic acid.

暴露于全反式视黄酸的母体小鼠腭裂胎儿的次级腭中 DNA 甲基化发生变化。

• 期刊: (Submitted)
• 作者: Kuriyama M;Udagawa K;Yoshimoto S;Ichinose M;Shiota K;Sato K;Koji Yamazaki;Matsuno Y;Mori C
• 通讯作者: Mori C