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Temperature-dependency of pacemaker activity in gastric smooth muscle

胃平滑肌起搏器活性的温度依赖性

基本信息

批准号：
17590190
负责人：
SUZUKI Hikaru
金额：
$ 2.24万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590190/
关键词：
Gastris smooth muscle Pacemaker activity Temperature-dependency Interstitial cells of Cajal Slow wave Mitochondria Metabolic activity

项目摘要

In isolated gastric smooth muscle tissue of the guinea-pig, the effects of changes in temperature and their modulation by metabolic inhibitors on slow waves were investigated, to understand the possible involvement of metabolic processes in the pacemaker activity of spontaneous activity of gastric smooth muscle tissues. Experiments were carried out to record spontaneously generating electrical responses from circular and longitudinal smooth muscle cells and interstitial cells of Cajal, in small pieces of gastric smooth muscle tissues isolated from the guinea-pig, during changing temperature between 24 and 42 ℃. With elevation of temperature, the frequency and the speed of rate of rise were increased in slow waves, follower potentials and pacemaker potentials, while the duration of these potentials was reduced. These changes were related with the elevation of temperature in a linear way. The latency for generating the 2nd component of slow wave was decreased in an exponential way betwee … More n 24 and 32 ℃, while it remained unchanged above 32 ℃. The resting membrane potential and amplitude of slow waves were not sensitive to temperature. Calculation of temperature co-efficiency (Q10 value) indicated that it was higher on the frequency, duration and rate of rise of slow waves. Potassium cyanide (KCN), an inhibitor of mitochondrial metabolic activity, inhibited only the changes in frequency, but not the duration and rate of rise time, of slow waves in response to temperature. These results suggested that the factors determining the frequency of slow waves alone are causally related to the mitochondrial metabolic activity. As the duration and the rate of rise time of slow waves were not altered by KCN, possible contribution of factors other than mitochondrial metabolism was considered for their determination. The regulation of the activity of entire stomach might be the most active region, and it was found to be the corpus region of the stomach. The corpus region produced slow waves 4-6 times every min, and they had strong potential-dependency, suggested a possible involvement of factors other than mitochondria for regulation of the activity. Less

在离体豚鼠胃平滑肌组织中，研究了温度变化及其代谢抑制剂对慢波的调节作用，以了解代谢过程可能参与胃平滑肌组织自发活动的起搏活动。在24 ~ 42 ℃变温条件下，记录了离体豚鼠小块胃平滑肌组织中的环、纵平滑肌细胞和Cajal间质细胞自发产生的电反应。随温度升高，慢波、跟随电位和起搏电位的频率和上升速率增加，而持续时间缩短。这些变化与温度的升高呈线性关系。慢波第二成分的潜伏期呈指数下降， ...更多信息 n 24和32 ℃时，而在32 ℃以上则无变化。静息膜电位和慢波振幅对温度不敏感。温度系数（Q10值）的计算表明，慢波的频率、持续时间和上升速率都较高。氰化钾（KCN），一种线粒体代谢活性的抑制剂，只抑制频率的变化，但不抑制的持续时间和上升时间的速度，响应于温度的慢波。这些结果表明，决定慢波频率的因素单独与线粒体代谢活动有因果关系。由于KCN未改变慢波的持续时间和上升时间速率，因此考虑了线粒体代谢以外因素的可能贡献。整个胃的活动调节可能是最活跃的区域，它被发现是胃体区。体区每分钟产生4-6次慢波，并有较强的电位依赖性，提示除线粒体外，可能还有其他因素参与调节。少