Characterizing the sympathetic premotor neurons participating in thermoregulatory responses

表征参与温度调节反应的交感前运动神经元

基本信息

批准号：
17590199
负责人：
SAIGUSA Takeshi
金额：
$ 2.24万
依托单位：
University of Yamanashi
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

1. In urethane-anesthetized spontaneously-breathing rabbits, activities of sympathetic premotor neurons in the raphe nuclei were recorded extracellularly to investigate the effects of thermal stimuli applied to the preoptic area and anterior hypothalamus ( PO/AH ) on the raphe neurons. Some neurons were activated by cold stimuli and inhibited by warming. The responses were well correlated with the changes in ear skin temperature, suggesting that these neurons were involved in the cutaneous vasoconstrictor pathway. Other neurons were inhibited by cooling and activated by warming. Although the role of these neurons was not clarified, it is possible that they are involved in the sympathetic pathway innervating visceral blood vessels, because visceral blood flow responds reciprocally to cutaneous one during thermal stimuli. Some of the neurons responding to thermal stimuli had phasic activities synchronized to cardiac and/or respiratory cycles, suggesting that baroreceptor and respiratory signals, as well as thermoregulatory signals, are converged to the raphe nuclei.2. A new spike-sorting software was developed to improve separation of multi-unit activities. In this system, all the signals crossing a threshold are analyzed to get 12 kinds of peak and valley parameters. Any pair of these parameters can be displayed as a x-y scatter plot, on which any number of spike-sorting area can be defined. Based on the scatter plot, 2-dimensional histogram can also be displayed as a 3-dimensional surface plot, which makes easier to define sorting areas and to find out overlapped area of multiple units. As a result, the system improves quality of both single-unit recordings and simultaneous multi-unit recordings.

1。在尿烷 - 动理的自发呼吸兔中，细胞外记录了raphe核中交感神经前神经元的活性，以研究应用于前下区域和前丘脑（PO/PO/AH）对raphe神经元的热刺激的作用。一些神经元被冷刺激激活，并被变暖抑制。这些反应与耳朵皮肤温度的变化很好地相关，表明这些神经元参与皮肤血管收缩途径。其他神经元被冷却抑制，并通过变暖激活。尽管这些神经元的作用尚未澄清，但它们可能参与了神经内脏血管的交感神经途径，因为内脏的血液流动在热刺激过程中对皮肤的血液流动反应。对热刺激反应的一些神经元具有与心脏和/或呼吸周期同步的阶段性活性，这表明压力感受器和呼吸信号以及热调节信号融合到Raphe Nuclei.2。开发了一种新的尖峰分类软件，以改善多单元活动的分离。在此系统中，分析了跨越阈值的所有信号以获得12种峰值和山谷参数。这些参数的任何一对都可以显示为X-Y散点图，可以在其上定义任何数量的尖峰区域。基于散点图，二维直方图也可以显示为3维表面图，这使得更易于定义排序区域并找出多个单元的重叠区域。结果，该系统提高了单单元录制和同时的多单元录音的质量。