Ubiquitination of overexpressed protein and enclosure by inducible ER membrane

据报道，在培养细胞中过表达内质网(ER)膜蛋白，如微粒体乙醛脱氢酶(ALDH)，会导致特定的ER膜结构(晶态ER，CER)的诱导。我们最近首次发现，一种与ER无关的线粒体丝氨酸前体：丙酮酸氨基转移酶(SPT45K)的过表达诱导了CER的产生，而其他线粒体前体蛋白POTC和PAD的过表达也诱导了ER聚集。我们还发现，在C端具有ALDH的ER靶向序列的嵌合荧光蛋白通过过度表达具有显著的ER聚集活性。由于在缺乏细胞器靶向信号的蛋白质(SPT43K、ALDHdC、EYFP)中没有观察到CER(或ER聚集体)的诱导活性，因此其活性依赖于它们的结构。进一步表明，CER(ER聚集体)的诱导活性依赖于它们在细胞中的表达水平。这些数据表明，通过诱导新的内质网膜结构，该细胞具有一种新的系统来检测细胞质中表达的蛋白质的质量(结构)和数量。

It has been reported that overexpression in the culture cells of endoplasmic reticulum (ER) membrane proteins, such as microsomal aldehyde dehydrogenase (ALDH), causes the induction of a specific ER membrane structure (crystalloid ER, cER). We recently found for the first time that overexpression of an ER-unrelated, mitochondrial precursor for serine : pyruvate aminotransferase (SPT45K) induced the generation of cER, and overexpressed pOTC and pAd, other mitochondrial precursor proteins, also induced the ER aggregates. We also found that the chimeric fluorescent protein having the C terminal ER targeting sequence of ALDH at the C terminus had the remarkable ER aggregating activity by the overexpression. Because these cER (or ER aggregates) inducing activities were not observed in the cases of proteins (SPT43K, ALDHdC, EYFP) lacking organelle targeting signals, the activities is dependent on their structures. It was further indicated that the cER (ER aggregates) inducing activities was dependent on their expression levels in the cell. These data suggest that the cell has a novel system for the detection of the quality (structure) and the quantity of proteins expressed in the cytoplasm by inducing the new ER membrane structure.