ANALYSIS OF TWO PROMOTERS AND 5'FLANKING REGION OF RAT SERINE : PYRUVATE AMINOTRANSFERASE GENE

大鼠丝氨酸丙酮酸转氨酶基因的两个启动子和5侧翼区的分析

基本信息

  • 批准号:
    05680546
  • 负责人:
  • 金额:
    $ 1.28万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1993
  • 资助国家:
    日本
  • 起止时间:
    1993 至 1994
  • 项目状态:
    已结题

项目摘要

(1) Sequence required for the basal transcriptional activity of two SPT gene promotersA single SPT gene has two promoters, the upstream promoter (transcribed from+1) which has TATA box and whose activity is enhanced by cAMP administration and the downstream promoter (transcribed from +66) which contains no TATA box and whose activity is not affected by cAMP.To identify the regions necessary for the basal transcription by these two promoters, I constructed many 5'-deleted recombinant plasmids whose 3'-ends are +36 for the upstream promoter or +106 for the downstream promoter. The transcription from the upstream initiation site was reached to a maximum level in a plasmid containing a sequence from -192 to +36 of SPT gane, suggesting that, in addition to CCAAT box and TATA box which are located between -103 and +36 ; some element (s) contained between -192 and -103 is necessary for the maximum activity. The transcriptional activity of the downstream promoter, on the other hand, was detect … More ed in a plasmid having a sequence from +36 to +106 and was reached to a maximum level in a plasmid containing a sequence from -51 to +106. This suggests that some element contained between -51 and +11 are necessary for the maximum transcriptional activity of the downstream promoter even if HIP1 (Housekeeping initiation protein 1), which is proposed as one of TATA-less promoters and is located between +11 and +106, functions as the downstream promoter.(2) Transcriptional regulation and cell-specific expression of SPT geneIt has been indicated that the region downstream from -103 contributes to the enhancement of SPT gene by cAMP rather than CRE (cAMP responsive element) located between -684 and -621. This enhancement was observed only in HepG2 cells, not in HeLa, CHO-K1, CV-1 and COS-1 cells. Because the transcriptional enhancement of SPT gene by cAMP is repressed by cycloheximide, a inhibitor of protein synthesis, it seems that CREB (CRE binding protein) first increases the liver-specific expression of some transcriptional factor and then the translation factor acts on the proximal region of SPT gene to cause the enhancement of SPT gene transcription. Less
(1)两个SPT基因启动子的基本转录活性所需的序列单个SPT基因有两个启动子,上游启动子(由+1转录而来,其活性可被cAMP增强)和下游启动子(由+66转录而来),不含TATA盒,其活性不受cAMP的影响。为了确定这两个启动子基础转录所必需的区域,我构建了许多5‘端缺失的重组质粒,其3’端分别为上游启动子+36或下游启动子+106。在含有-192~+36序列的质粒中,上游起始点的转录达到最高水平,这表明,除了位于-103~+36之间的CCAAT box和TATA box外,含有-192~-103之间的某些元素(S)是达到最大活性所必需的。另一方面,检测到下游启动子的转录活性为…在具有+36到+106序列的质粒中更多,并且在包含-51到+106序列的质粒中达到最大水平。这表明,即使位于+11和+106之间的无TATA启动子之一的HIP1作为下游启动子发挥作用,下游启动子的最大转录活性也需要-51到+11之间的某些元件。(2)SPT基因的转录调控和细胞特异性表达已经表明,-103下游的区域有助于cAMP而不是位于-684和-621之间的CRE(cAMP反应元件)对SPT基因的增强。这种增强作用仅在HepG2细胞中观察到,而在HeLa、CHO-K1、CV-1和COS-1细胞中未观察到。由于cAMP对SPT基因的转录增强作用受蛋白质合成抑制剂放线菌亚胺的抑制,CREB(CRE结合蛋白)可能首先增加某些转录因子的肝脏特异性表达,然后翻译因子作用于SPT基因的近端,从而导致SPT基因转录的增强。较少

项目成果

期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Oda,T: "Characterization and sequence analysis of rat serine:pyruvate/alanine:glyoxylate aminotransferase gene" Genomics. 17. 59-65 (1993)
Oda,T:“大鼠丝氨酸:丙酮酸/丙氨酸:乙醛酸转氨酶基因的表征和序列分析”基因组学。
  • DOI:
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Uchida,C: "Regulation by glucagon of serine:pyruvate/alanine:glyoxylate aminotransferase gene expression in cultured rat hepatocytes" J.Biol.Chem.269. 8849-8856 (1994)
Uchida,C:“培养的大鼠肝细胞中丝氨酸:丙酮酸/丙氨酸:乙醛酸转氨酶基因表达的胰高血糖素调节”J.Biol.Chem.269。
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  • 影响因子:
    0
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  • 通讯作者:
Ohbayashi,K.: "Characterization of transcription from the downstream start site of the rat serine:pyruvate/alanine:glyoxylato aminotransferase gone" Biomed.Res.(in press). (1995)
Ohbayashi,K.:“大鼠丝氨酸下游起始位点的转录特征:丙酮酸/丙氨酸:乙醛酸转氨酶消失”Biomed.Res.(正在出版)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Sato,S: "Quantitative cryoimmunogold electron microscopic studies on induction of serine:pyruvate aminotransferase in rat liver mitochondria by administration of glucagon" Cell Struct.Funct.(印刷中). (1995)
Sato, S:“通过施用胰高血糖素对大鼠肝线粒体中丝氨酸:丙酮酸转氨酶的诱导进行定量冷冻免疫金电子显微镜研究”Cell Struct.Funct(出版中)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Oda,T.: "Characterization and sequence analysis of rat serine:pyruvate/alanine:glyoxlate aminotransferase gene" Genomics. 17. 59-65 (1993)
Oda,T.:“大鼠丝氨酸:丙酮酸/丙氨酸:乙醛酸转氨酶基因的表征和序列分析”基因组学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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ODA Toshiaki其他文献

ODA Toshiaki的其他文献

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{{ truncateString('ODA Toshiaki', 18)}}的其他基金

A comparative study on morphology and functions of muscle-tendon complex in Japanese and Kenyan distance runners
日本与肯尼亚长跑运动员肌腱复合体形态与功能的比较研究
  • 批准号:
    23500729
  • 财政年份:
    2011
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Determination of local stress and deformation in human skeletal muscles using a combined approach of in vivo experiments with computer simulation.
使用体内实验与计算机模拟相结合的方法确定人体骨骼肌的局部应力和变形。
  • 批准号:
    19700519
  • 财政年份:
    2007
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Ubiquitination of overexpressed protein and enclosure by inducible ER membrane
过度表达蛋白的泛素化和诱导 ER 膜的封装
  • 批准号:
    17590244
  • 财政年份:
    2005
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of mechanism for selective translocation of a protein having two subcellular localization signals
具有两个亚细胞定位信号的蛋白质选择性易位机制分析
  • 批准号:
    10480165
  • 财政年份:
    1998
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
REGULATION MECHANISM BY HORMONES OF THE TRANSCRIPTION OF RAT SERINE:PYRUVATE AMINOTRANSFERASE GENE
激素对大鼠丝氨酸丙酮酸转氨酶基因转录的调控机制
  • 批准号:
    03680165
  • 财政年份:
    1991
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
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