Prognostic prediction in lupus nephritis using CARP which is newly identified as a podocyte marker.

使用 CARP 来预测狼疮性肾炎的预后，CARP 是新确定的足细胞标记物。

• 批准号: 17590306
• 负责人: MATSUURA Keiko
• 金额: $ 2.24万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590306/
• 关键词: CARP; lupus nephritis; proteinuria; renal function; LPS; angiotensin II; adriamycin; ARB; adrialnycin

Cardiac ankyrin-repeated protein (CARP) was originally identified as a protein specifically expressed in cardiomyocytes, but has recently been found to be up-regulated in some muscle diseases including muscular dystrophy and myopathy, suggesting that CARP may be induced in some pathological conditions.In this study, we immunohistochemically analyzed renal biopsy samples from patients with glomerular diseases and two individuals with normal kidney. We found that CARP was expressed in renal podocytes at a high level in crescentic glomerulonephritis (GN), diabetic nephropathy (DN), and lupus nephritis (LN), although it was not expressed in normal kidney. Interestingly, in LN, CARP expression tended to be induced in cases exhibiting nephrotic syndrome, but less so in cases without nephrotic syndrome, suggesting that CARP expression is correlated with the severity of proteinuria.Then we analyzed the expression of CARP in podocytes of Balb/c mice that had been administrated with LPS or adria … More mycin. When LPS and adriamycin were injected to mice, CARP was induced in glomerular cells that co-express synaptopodin, a marker for podocyte, whereas CARP was not induced in mice injected with PBS. Thus, it is strongly suggested that CARP is really induced in podocytes in response to podocyte-injury. Rat glomerular parietal epithelial cell (PEC) underwent morphological changes and exhibited membrane ruffling after treatment with LPS and angiotensin II. Interestingly, phalloidin staining detected accumulation of F-actin in these areas of membrane ruffling When we treated with anagiotensin receptor blocker (ARB), morphological and distributional changes were partially blocked. On the other hand, we immunohistochemically analyzed the CARP-positive cells in urine frojm patients of GN. We could detect a small number of CARP-positive cells. Although we also tried to identify a monoclonal antibody against CARP, we have not identified it yet.In conclusion, it is suggested that immunohistochemical evaluation of CARP in biopsy samples or urine from patients with LN may be useful for diagnosis of disease severity, and probably for prediction of outcome in LN-patients. Less

心脏锚定重复蛋白（Cardiac anklost-repeated protein，CARP）最初被鉴定为在心肌细胞中特异表达的蛋白质，但最近发现在一些肌肉疾病中表达上调，包括肌营养不良和肌病，提示CARP可能在某些病理条件下被诱导。我们发现CARP在新月体肾小球肾炎（GN）、糖尿病肾病（DN）和狼疮性肾炎（LN）的肾足细胞中有高水平表达，而在正常肾脏中不表达。有趣的是，在LN中，CARP的表达倾向于在有肾病综合征的病例中被诱导，而在没有肾病综合征的病例中较少，这表明CARP的表达与蛋白尿的严重程度相关。 ...更多信息 霉素。当将LPS和阿霉素注射到小鼠中时，在共表达足细胞标记物突触足蛋白的肾小球细胞中诱导CARP，而在注射PBS的小鼠中不诱导CARP。因此，强烈表明CARP确实是在足细胞中响应足细胞损伤而诱导的。大鼠肾小球壁上皮细胞（PEC）在LPS和血管紧张素II处理后发生形态学改变，并出现膜皱褶。有趣的是，鬼笔环肽染色检测到F-actin在这些膜皱褶区域的积累。当我们用血管紧张素受体阻断剂（ARB）处理时，形态和分布的变化被部分阻断。另一方面，我们对例肾小球肾炎患者尿液中的CARP阳性细胞进行了化学分析。我们可以检测到少量的CARP阳性细胞。虽然我们也试图确定一种单克隆抗体对CARP，我们还没有确定它you.In结论，这表明，免疫组化评价CARP活检标本或尿液LN患者可能是有用的疾病的严重程度的诊断，并可能为LN患者的预后预测。少

项目成果

Acase of right isomerism showing long survival without surgery.

右侧异构病例显示无需手术即可长期生存。

• 发表时间: 2007
• 期刊: Southern Medical Journal 100
• 作者: Matsuura K;Akizuki S;Nakamura N;Ishibashi-Ueda H;Moriyama M
• 通讯作者: Moriyama M

ARPP protein is selectively expressed in renal oncocytoma, but not in renal cell carcinomas

ARPP 蛋白在肾嗜酸细胞瘤中选择性表达，但在肾细胞癌中不表达

• 发表时间: 2007
• 期刊: Modern Pathology 20
• 作者: Shomori K.;Nagashima Y.;Kuroda M.;Takeda A.;Tsukamoto Y.;Kawamura T.;Hiramatsu T.;Matsuura K.;Hijiya N.;Yano S.;Yokoyama S.;Ito H.;Moriyama M.
• 通讯作者: Moriyama M.

Extracellular signal-regulated protein kinase is activated in cervical intraepithelial neoplasms but inactivated in invasive cervical carcinoma.

细胞外信号调节蛋白激酶在宫颈上皮内肿瘤中被激活，但在浸润性宫颈癌中失活。

• 发表时间: 2006
• 期刊: Pathology International 56
• 作者: Matsuura K.;Nohno Y.;Hijiya N.;Uchida T.;Tsukamoto Y;Moriyama M.
• 通讯作者: Moriyama M.

Extracellular signal-regulated protein kinase is activated in cervical intraepithelial neoplasms but inactivated in invasive cervical carcinoma

细胞外信号调节蛋白激酶在宫颈上皮内肿瘤中激活，但在浸润性宫颈癌中失活

• 发表时间: 2006
• 期刊: Pathology International 56
• 作者: Matsuura K.;Nohno Y.;Hijiya N.;Uchida T.;Tsukamoto Y.;Moriyama M.
• 通讯作者: Moriyama M.

A case of spindle cell lipoma arising in the tongue.

舌部梭形细胞脂肪瘤一例。

• 发表时间: 2005
• 期刊: Oral Oncology Extra 41(10)
• 作者: Matsuura K.;Akizuki S.;Hirano K.;Yanagisawa S.;Moriyama M.
• 通讯作者: Moriyama M.