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Prediction of minute prostatic cancer development by telomere length measured with tissue section FISH method.

通过组织切片 FISH 方法测量的端粒长度预测微小前列腺癌的发展。

基本信息

批准号：
17590325
负责人：
IZUMIYAMA Naotaka
金额：
$ 2.3万
依托单位：
Tokyo Metropolitan Foundation for Research on Aging and Promotion of Human Welfare
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590325/
关键词：
Prostate Telomere Telomerase FISH Pathology 核酸癌ゲノム細胞・組織

项目摘要

We invited Dr. Steven Poon from the BC Cancer Center, Canada, twice in 2005 and 2006, to work as a member of our research team. In collaboration with Dr. Poon, we devised a new software program, "Tissue Telo version 2", for measurement of telomere length using tissue sections. With this approach, and the use of fluorescence in situ hybridization (FISH) with a telomere-specific probe on tissue sections, we were able to measure telomere length in archival sections of prostatic tissue, and obtained the following data :1.Difficulties with analysis of prostatic tissueProstatic tissue contains rough muscle and collagen fibers, which have very strong autofluorescence. This autofluorescence prevented us from measuring telomere length in epithelium and stroma. Although several methods for removing this autofluorescence were tried, sufficiently satisfactory fluorescence microscopy images could not be obtained. Thus it is more difficult to obtain good images using prostate than with gastrointestinal tissue.2.Improvement of quantitative fluorescence in situ hybridizationWe used centromere fluorescence intensity as an internal control, and expressed telomere length as the telomere/centromere intensity ratio (TCR). Moreover, we used TCR of cell block sections of TIG-1 to obtain more precise data. The normalized TCR was the TCR of a histologic section divided by the TCR of a section of TIG-1 from a cell block.3.TCRs of carcinoma cells were 71-83% those of normal fibroblasts. On the basis of these different TCRs, it appeared feasible to distinguish cancer cells from fibroblasts.4.TCRs of (luminal cells) were smaller than those of (basal cells) in the prostate gland. It is suggested that luminal and basal cells constitute different populations in the prostate.5.TCRs of normal fibroblasts were different from those of cancer fibroblasts. It is suggested that these cell populations in the stroma are different.

2005年和2006年，我们两次邀请加拿大BC癌症中心的Steven Poon博士作为我们研究团队的一员。我们与潘博士合作，设计了一个新的软件程序，“Tissue Telo version 2”，用于使用组织切片测量端粒长度。利用这种方法，并使用荧光原位杂交（FISH）与端粒特异性探针在组织切片上，我们能够测量端粒长度的前列腺组织的档案切片，并获得了以下数据：1.前列腺组织分析的困难前列腺组织包含粗糙的肌肉和胶原纤维，它们具有非常强的自发荧光。这种自发荧光使我们无法测量上皮和间质中的端粒长度。尽管尝试了几种去除这种自发荧光的方法，但不能获得足够令人满意的荧光显微镜图像。2.荧光原位杂交定量方法的改进以着丝粒荧光强度为内对照，端粒长度以端粒/着丝粒强度比（TCR）表示。此外，我们使用了TIG-1细胞块切片的TCR，以获得更精确的数据。标准化TCR是组织切片的TCR除以来自细胞块的TIG-1切片的TCR。3.癌细胞的TCR是正常成纤维细胞的71-83%。根据这些不同的TCR，区分癌细胞和成纤维细胞似乎是可行的。4.前列腺中（腔细胞）的TCR小于（基底细胞）的TCR。提示前列腺组织中管腔细胞和基底细胞构成不同的细胞群。5.正常前列腺成纤维细胞TCR与癌成纤维细胞TCR不同。这表明，这些细胞群在基质中是不同的。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Telomere length variations in 6 mucosal cell types of gastric tissue observed using novel Q-FISH method

使用新型 Q-FISH 方法观察胃组织 6 种粘膜细胞类型的端粒长度变化

DOI：
发表时间：
2007
期刊：
Human Pathology in press
影响因子：
0
作者：
Aida J;Izumiyama-Shimomura N;Nakamura K;Ishii A;Ishikawa N;Honma N;Kurabayashi R;Kammori M;Poon SSS;Arai T;Takubo K
通讯作者：
Takubo K

Telomere shortening with aging in the human pancreas

DOI：
10.1016/j.exger.2006.06.036
发表时间：
2006-09-01
期刊：
EXPERIMENTAL GERONTOLOGY
影响因子：
3.9
作者：
Ishii, Akio;Nakamura, Ken-Ichi;Takubo, Kaiyo
通讯作者：
Takubo, Kaiyo

Expression of human telomerase reverse transcriptase gene and protein, and of estrogen and progesterone receptors, in breast tumors : Preliminary data from neo-adjuvant chemotherapy.

乳腺肿瘤中人端粒酶逆转录酶基因和蛋白质以及雌激素和孕激素受体的表达：新辅助化疗的初步数据。

DOI：
发表时间：
2005
期刊：
Int J Oncol 27
影响因子：
0
作者：
Kammori M;Izumiyama N;Hashimoto M;Nakamura K;Okano T;Kurabayashi R;Naoki H;Honma N;Ogawa T;Kaminishi M;Takubo K.
通讯作者：
Takubo K.

Specific subtelomere loss on chromosome der (11) t(3 ; 11) (q23 ; q23) x2 in anaplastic thyroid cancer cell line OCUT-1.

间变性甲状腺癌细胞系 OCUT-1 中染色体 der (11) t(3 ; 11) (q23 ; q23) x2 上的特异性亚端粒丢失。