Effects of perinatal combined exposure to organic pollutants on rat offspring

研究了妊娠期和哺乳期摄入三丁基锡（TBT）、1,4-二氯苯（DCB）和p， p'-DDE （DDE）的母鼠后代围产期联合暴露对TBT、DCB和DDE的影响。水坝分别分为三组；即对照组、TBT或DCB组和TBT或DCB+DDE组。对照组大鼠饲喂正常大鼠饲料，CE-2、DCB组大鼠给予含有25ppm (2 mg/kg) TBT或DCB的CE-2， TBT或DCB+DDE组大鼠给予含有25ppm TBT或DCB和125ppm (10 mg/kg) DDE的CE-2。从妊娠第1天至产后第21天，饲喂预拌饲料42 d。为避免饲养状态导致的生长差异，在出生后第2 ~ 4天对仔鼠进行雄性和雌性的扑杀。断奶后给子代大鼠喂食CE-2。经处理的母鼠妊娠和生殖结局未见恶化。围产期联合暴露于TBT使大鼠子代发育迟缓，而暴露于DCB使雌性子代胸腺保存。然而，这些作用被同时暴露于DDE所抵消。此外，本实验还观察到暴露于DCB和DDE的协同效应，发现卵巢重量下降，可能导致生殖功能障碍。

Effects of perinatal combined exposure to tributyltin (TBT) or 1,4-dichlorobenzene (DCB), and p, p'-DDE (DDE) were investigated in rat offspring of dams ingesting TBT or DCB, and DDE during the gestational and lactational period. Dams were divided into three groups, respectively; namely control group, TBT or DCB group and TBT or DCB+DDE group. Control group rats were fed normal rat chow, CE-2,DCB group rats were given CE-2 containing 25ppm (2 mg/kg) TBT or DCB, and TBT or DCB+DDE group rats were administered CE-2 containing 25ppm TBT or DCB and 125ppm (10 mg/kg) DDE. Dams were fed prepared feed for 42 days from gestational day 1 to postpartum day 21. Rat offspring were culled on postnatal day 2 to 4 males and females each, in order to avoid differences of growth due to feeding states. Rat offspring were fed CE-2 after weaning. No deteriorated pregnancies and reproductive outcomes were observed in treated dams. Perinatal combined exposure to TBT produced growth retardation in rat offspring and that to DCB induced thymus preservation in female offspring. However, these actions were counterbalanced by concurrent exposure to DDE. Furthermore, synergistic effects of exposure to DCB and DDE were observed and the reduction of the ovary weight was seen in this experiment, which might lead to reproductive dysfunction.