The treatment against respiratory infection -gene therapy with SiRNA-

呼吸道感染的治疗-SiRNA基因治疗-

• 批准号: 17590796
• 负责人: YANAGIHARA K.
• 金额: $ 1.22万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590796/
• 关键词: respiratory infection; gene therapy; SiRNA; pathogenesis; pneumococcus; Pseudomonas aeruginosa; MRSA

The anti-PcrV IgG reduced excessive inflammatory reactions, such as the recruitment of inflammatory cells and the production of pro-inflammatory cytokines, caused by chronic P.aeruginosa respiratory infection. These data suggest that treatment with anti-PcrV IgG may be useful in chronic P.aeruginosa respiratory infection. (Imamura Y, Yanagihara K, et al. Eur.Respir.J. 2007 in press)Telithromycin reduced the number of viable bacteria by inhibiting biofilm formation in the absence of an anti-inflammatory effect. The present data also indicated a difference between the effects of macrolides and ketolides against chronic respiratory infections. (Yanagihara K, et al. Chemotherapy 2007;53:10-13)Sivelestat prolonged survival in a severe pneumococcal pneumonia model. A possible explanation for the improved survival is that sivelestat prevents tissue damage by inhibiting NE activity in the lung. As a result of this reduced damage, the number of viable bacteria recovered from blood is lower in the sivelestat group than in the control group. Thus, improved mortality rates were seen with sivelestat treatment. NE inhibitors may therefore be useful in treating with patients with severe pneumonia. (Yanagihara K, et al. Experimental Lung Research, Mar;33(2):71-80)

抗PcrV IgG减少了由慢性铜绿假单胞菌呼吸道感染引起的过度炎症反应，例如炎症细胞的募集和促炎细胞因子的产生。这些数据表明，用抗PcrV IgG治疗慢性铜绿假单胞菌呼吸道感染可能是有用的。（Imamura Y，Yanagihara K等，Eur.Respir.J.2007出版中）泰利霉素通过在不存在抗炎作用的情况下抑制生物膜形成来减少活细菌的数量。目前的数据还表明大环内酯类和酮内酯类对慢性呼吸道感染的作用存在差异。（Yanagihara K等人，Chemotherapy 2007;53：10-13）西维来司他延长了严重肺炎球菌肺炎模型中的存活。存活率提高的一个可能解释是sivelestat通过抑制肺中的NE活性来防止组织损伤。由于这种减少的损害，从血液中回收的活细菌的数量在西维来司组中低于对照组。因此，sivelestat治疗可改善死亡率。因此，NE抑制剂可用于治疗患有严重肺炎的患者。（Yanagihara K等，实验肺研究，3月;33（2）：71-80）

项目成果

Effects of macrolides against pneumolysin of macrolide-resistant Streptococcus pneumoniae.

大环内酯类药物对耐大环内酯类肺炎链球菌肺炎球菌溶血素的影响。

• 发表时间: 2006
• 期刊: Eur Respir J in press
• 作者: Fukuda Y;Tsukamoto K;et al.
• 通讯作者: et al.

Role of Pseudomonas aeruginosa quorum-sensing systems in a mouse model of chronic respiratory infection

• DOI: 10.1099/jmm.0.46004-0
• 发表时间: 2005-06-01
• 期刊: JOURNAL OF MEDICAL MICROBIOLOGY
• 影响因子: 3
• 作者: Imamura, Y;Yanagihara, K;Kohno, S
• 通讯作者: Kohno, S

Effects of short interfering RNA against methicillin-resistant Staphylococcus aureus coagulase in vitro and in vivo

• DOI: 10.1093/jac/dki416
• 发表时间: 2006-01-01
• 期刊: JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
• 影响因子: 5.2
• 作者: Yanagihara, K;Tashiro, M;Kohno, S
• 通讯作者: Kohno, S

Effects of specific neutrophil elastase inhibitor, sivelestat sodium hydrate, in murine model of severe pneumococcal pneumonia.

特异性中性粒细胞弹性蛋白酶抑制剂西维来司他钠水合物对严重肺炎球菌肺炎小鼠模型的影响。

• 发表时间: 2007
• 期刊: Exp Lung Re 33
• 作者: Yanagihara K;Fukuda Y;Masafumi Seki;et. al.
• 通讯作者: et. al.

Effects of anti-PcrV Antibody in Murine Chronic Airway Pseudomonas aeruginosa Infection Model

抗PcrV抗体对小鼠慢性气道铜绿假单胞菌感染模型的影响

• 发表时间: 2007
• 期刊: European Repiratory Journal 29巻(In press)
• 作者: Imamura Y;Yanagihara K;et al.
• 通讯作者: et al.