The study of relation between behavior abnormalities and biomolecular abnormalities in intrauterine growth retardation rats

背景：为了阐明宫内生长迟缓(IUGR)婴儿脑损伤的机制，我们对胎儿/新生大鼠进行了脑内生物分子研究。材料和方法：用合成血栓素A2(STA2)给母鼠灌胃诱导IUGR模型大鼠。采用原位末端标记法(TUNEL)和免疫印迹法(Western Blotting)分别对大鼠脑内细胞凋亡进行组织学和定量研究。结果：IUGR组和对照组新生儿平均出生体重分别为4.73±0.33g和5.95±0.35g。IUGR组较对照组有更多的细胞凋亡。而bcl2、bax和β-肌动蛋白在两组间的表达差异无统计学意义。在免疫组织学研究中，IUGR大鼠神经胶原蛋白、磷酸多糖和神经多糖的表达均升高。免疫印迹分析显示IUGR大鼠神经钙素、磷脂酰胆碱的表达均升高。结论：本研究结果提示CSPG可能与IUGR婴儿的脑损害有关。这些发现为阐明IUGR的脑损伤提供了关键。

Background : To elucidate the mechanism of brain insults in intrauterine growth retardation (IUGR) infant, we conducted the bio-molecular studies in the brain of fetal/neonatal rats.Materials and Methods : IUGR model rats were induced by synthetic thromboxane A2 (STA2) administration to maternal rats. We evaluated apoptosis in the rat brain histologically and quantitatively by using TUNEL and western blotting, respectively. The expressions of chondroitin sulfate proteoglycan (CSPG), such as Neurocan, Phosphacan and Neuroglycan, were evaluated by immuno-histochemical methods and western blotting methodsResults : The mean birth weight in IUGR and control was 4.73 +/- 0.33g and 5.95 +/- 0.35g, respectively. There were more apoptotic cell numbers in the IUGR compared to in control. However, there was not difference in the expression of BCL-2, BAX and β-actin between two groups. In the immuno-histological study, the expression of Neurocan, Phosphacan and Neuroglycan were elevated in IUGR rats. Also, in the western blotting analysis, the expression of Neurocan, Phosphacan were elevated in IUGR rats. The localization of CSPG was not different between two groups.Conclusions : The results in this study suggested that CSPG might be associated with the brain insult in IUGR infants. These findings provide the key of the elucidation of brain insults in IUGR.