The study of relation between behavior abnormalities and biomolecular abnormalities in intrauterine growth retardation rats

背景资料：为探讨宫内发育迟缓（IUGR）新生儿脑损伤的机制，本研究对胎/新生大鼠脑组织进行了生物分子学研究。材料与方法：采用合成血栓素A_2（STA_2）诱发IUGR模型。采用TUNEL法和Western blotting法分别从组织学和定量两个方面对大鼠脑组织中的细胞凋亡进行了研究。结果：IUGR组和对照组的平均出生体重分别为4.73 ± 0.33g和5.95 ± 0.35g; IUGR组凋亡细胞数明显多于对照组。而BCL-2、BAX和β-actin的表达在两组间差异无统计学意义。免疫组化结果显示，IUGR大鼠脑组织中Neurocan、Phosphacan和Neuroglycan的表达明显升高。Western blotting分析显示，IUGR大鼠脑组织中Neurocan、Phosphacan表达增强。结论：CSPG可能与IUGR患儿的脑损伤有关。这些发现为阐明IUGR脑损伤的机制提供了关键。

Background : To elucidate the mechanism of brain insults in intrauterine growth retardation (IUGR) infant, we conducted the bio-molecular studies in the brain of fetal/neonatal rats.Materials and Methods : IUGR model rats were induced by synthetic thromboxane A2 (STA2) administration to maternal rats. We evaluated apoptosis in the rat brain histologically and quantitatively by using TUNEL and western blotting, respectively. The expressions of chondroitin sulfate proteoglycan (CSPG), such as Neurocan, Phosphacan and Neuroglycan, were evaluated by immuno-histochemical methods and western blotting methodsResults : The mean birth weight in IUGR and control was 4.73 +/- 0.33g and 5.95 +/- 0.35g, respectively. There were more apoptotic cell numbers in the IUGR compared to in control. However, there was not difference in the expression of BCL-2, BAX and β-actin between two groups. In the immuno-histological study, the expression of Neurocan, Phosphacan and Neuroglycan were elevated in IUGR rats. Also, in the western blotting analysis, the expression of Neurocan, Phosphacan were elevated in IUGR rats. The localization of CSPG was not different between two groups.Conclusions : The results in this study suggested that CSPG might be associated with the brain insult in IUGR infants. These findings provide the key of the elucidation of brain insults in IUGR.