A research on the dendritic cell activation in psoriasis

银屑病树突状细胞活化的研究

• 批准号: 17591188
• 负责人: HIGAKI Megumu
• 金额: $ 2.18万
• 依托单位: JIKEI UNIVERSITY (2006)St. Marianna University School of Medicine (2005)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591188/
• 关键词: Psoriasis; Keratinocytes; CD208; Dendritic cells; Differentiation; Lysosome; Immunohistochemistry; Toll様受容体; C型レクチン受容体; ケモカイン

Psoriasis is a chronic inflammatory skin disease characterized by epidermal hyperproliferation and infiltration of inflammatory leukocytes. Since the important role of innate immunity involving dendritic cells (DCs) and keratinocytes has been suggested in psoriasis, we immunohistochemically examined the expression of DC markers such as CD1a,CD83,CD207 (Langerin), CD208 (DC-LAMP), and CD209 (DC-SIGN) in psoriatic skin. Interestingly, CD208 was strongly expressed in suprabasal layer keratinocytes in addition to DCs in the perivasular lesions of the psoriatic dermis. Furthermore, the enhanced expression of CD208 in the perinuclear lesions of IFN-g-/TPA-stimulated keratinocytes was observed in vitro. Since a defect of the granular layer in psoriatic lesions has been recognized, increased expression of lysosome-related CD208 in the suprabasal keratinocytes of psoriatic lesions might represent aberrant epidermal differentiation. Additionally, these CD208-positive keratinocytes possessing antigen-processing activity might play a key role as antigen-presenting cells in psoriatic skin.

牛皮癣是一种慢性炎症性皮肤病，其特征是表皮过度增殖和炎性白细胞浸润。由于涉及树突状细胞 (DC) 和角质形成细胞的先天免疫在银屑病中发挥重要作用，我们采用免疫组织化学方法检测了银屑病皮肤中 DC 标记物的表达，例如 CD1a、CD83、CD207 (Langerin)、CD208 (DC-LAMP) 和 CD209 (DC-SIGN)。有趣的是，除了银屑病真皮血管周围病变中的 DC 外，CD208 在基底上层角质形成细胞中也强烈表达。此外，在体外观察到IFN-g/TPA刺激的角质形成细胞的核周损伤中CD208的表达增强。由于已经认识到银屑病病变中颗粒层的缺陷，因此银屑病病变的基底上角质形成细胞中溶酶体相关的 CD208 表达增加可能代表异常的表皮分化。此外，这些具有抗原加工活性的 CD208 阳性角质形成细胞可能作为银屑病皮肤中的抗原呈递细胞发挥关键作用。

项目成果

Ear involvement in patients with Rheumatoid Arthritis.

类风湿性关节炎患者的耳朵受累。

• 发表时间: 2005
• 期刊: Otol Neurotol 26
• 作者: Kitaichi Y;Inoue T;Izumi T;Nakagawa S;Kato A;Koyama T.;Kazuno AA.;Takatsu M etc.
• 通讯作者: Takatsu M etc.

Role of zinc in formation of PLGA/PLA nanoparticles encapsulating betamethasone phosphate and its release profile.

锌在包封磷酸倍他米松的 PLGA/PLA 纳米颗粒形成中的作用及其释放曲线。

• 发表时间: 2005
• 期刊: J Control Release 105
• 作者: Sugai T;Suzuki Y;Sawamura K;Fukui N;Inoue Y;Someya T;Ishihara T etc.
• 通讯作者: Ishihara T etc.

炎症性サイトカイン

炎症细胞因子

• 发表时间: 2006
• 期刊: リウマチ科 35・6
• 作者: Chen X;Niyonsaba F;Ushio H;Nagaoka I;Ikeda S;et al.;車地 暁生;Fang YR;Suzuki Y;Kaiya H;檜垣惠
• 通讯作者: 檜垣惠

Dioxin immunosensor using anti-2,3,7,8-TCDD antibody which was produced with mono 6-(2,3,6,7-tetrachloroxanthene-9-ylidene)hexylsuccinate as a hapaten.

二恶英免疫传感器使用抗 2,3,7,8-TCDD 抗体，该抗体是用单 6-(2,3,6,7-四氯呫吨-9-亚基)己基琥珀酸酯作为半抗原产生的。

• 发表时间: 2006
• 期刊: BIOSENS BIOELECTRON 22
• 作者: Jong-Won Park;Kurosawa S;Aizawa H;Hamano H;Harada Y;Asano S;Mizushima Y;Higaki M.
• 通讯作者: Higaki M.

高感度コア抗原を用いた各種インターフェロン治療効果予測の意義

使用高度敏感的核心抗原预测各种干扰素治疗效果的意义

• 发表时间: 2006
• 期刊: 医学と薬学 55・4
• 作者: Kitaichi Y;Inoue T;Nakagawa S;Izumi T;Koyama T;Yamamoto N et al.;Nishikawa T et al.;泉並木他
• 通讯作者: 泉並木他