Analysis of keratinocytes growth regulation by vitamine D3

维生素 D3 对角质形成细胞生长的调节分析

• 批准号: 09670899
• 负责人: HIGAKI Megumu
• 金额: $ 1.66万
• 依托单位: St.Marianna Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670899/
• 关键词: Psoriasis vulgaris; apaptosis; vitamine D3; keratinocytes; bcl-x; proliferation; Immunohistochemistry; RT-PCR; 尋常性乾癖

Psoriasis is a chronic skin disease characterized by epidermal hyperproliferation in addition to inflammatory cell infiltration. Hyperproliferation in psoriasis is regulated by various mediators including growth factors and/or apoptotic mechanism. In psoriatic lesional skin, innumerable apoptotic cells has been shown by TUNEL(TdT-mediated dUTP nick end labeling) method. However, the factors which regulate apoptosis of keratinocytes are unknown. In this study, we analyzed the expression of Bcl-x in normal and psoriatic skin immunohistochernically, as Bcl-x, one of the proteins in Bcl-2 family, is known to prevent apoptosis. Furthermore, to clarify the action mechanisms of vitamine D3, we compared the expression of Bcl and DNA fragmanetation in psoriatic lesion aftertopical application of vitamin D3 to those before the treatment.

银屑病是一种慢性皮肤病，其特征是表皮过度增殖，并伴有炎性细胞浸润。银屑病的过度增殖受到多种介质的调节，包括生长因子和/或凋亡机制。在银屑病皮损中，TdT介导的dUTP缺口末端标记法(TUNEL)显示出大量的凋亡细胞。然而，调控角质形成细胞凋亡的因素尚不清楚。在本研究中，我们用免疫组织化学方法分析了正常皮肤和银屑病患者皮肤中Bclx的表达，因为Bclx是Bcl2家族中已知的一种蛋白，它可以防止细胞凋亡。此外，为了阐明维生素D3的作用机制，我们比较了局部应用维生素D3后银屑病皮损中Bcl1的表达和DNA片段的变化。

项目成果

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Terajima S，Higaki M，Igarashi Y，Nogita T，Kawashima M.：“肿瘤坏死因子-a 在诱导牛皮癣粘附分子中的重要作用。”

Takahashi S,Inoue T,Higaki M,Mizushima Y: "Suppressive effects of the new anti-rheumatic drug KE-298 on TNF induced production of MMPs." Drug under Clinical Research. XXIV. 67-71 (1998)

Takahashi S、Inoue T、Higaki M、Mizushima Y：“新型抗风湿药 KE-298 对 TNF 诱导的 MMP 产生的抑制作用。”

Takahashi S, Inoue T, Higaki M, Mizushima Y: "Suppressive effects of the new anti-rheumatic drug KE-298 on TNF induced production of MMPs." Drug under Clinical Research. XXIV. 67-71 (1998)

Takahashi S、Inoue T、Higaki M、Mizushima Y：“新型抗风湿药 KE-298 对 TNF 诱导的 MMP 产生的抑制作用。”

Shimizu M,Higaki Y,Higaki M,kawashima M: "The role of ganzyme B-expressing CD8-positive T cells on apoptosis of keratinocytes in lichen planus" Arch Dermatol Res. 289. 527-532 (1997)

Shimizu M、Higaki Y、Higaki M、kawashima M：“表达 ganzyme B 的 CD8 阳性 T 细胞对扁平苔藓角质形成细胞凋亡的作用”Arch Dermatol Res。

Higaki M,Mizushima Y: "Detection of prion protein associated with Creutzfeld-Jakob disease." New Technology Japan. 26(5). 39-40 (1998)

Higaki M，Mizushima Y：“检测与克雅氏病相关的朊病毒蛋白。”