Establishment of breast cancer diagnosis system using alternative splicing detecting DNA microarray

基因组上排列的基因通过选择性剪接产生功能不同的基因。本文报道了雌激素受体剪接变异体在乳腺癌中的比例变化，雌激素受体剪接变异体的存在可能在乳腺癌的发生发展中起重要作用。本研究的目的是了解剪接变异体的表达模式，它与乳腺癌的发生、发展、转移、耐药的获得有关。我们希望建立基于基因的治疗系统，通过开发方法来定量分析改变的状态，使乳腺癌的诊断治疗成为可能。我们列出了128个基因，这些基因被报道与乳腺癌的发展有关或可能与乳腺癌的发展有关。每个基因选择1 ~ 61个寡核苷酸检测mRNA中外显子-外显子连接，在DNA微阵列芯片上共设置1447个寡核苷酸。我们最初计划使用梳状矩阵制作的DNA微阵列芯片，但由于公司的原因，我们无法将其用于一个问题，因此我们匆忙协商可以使用基因前沿的尖端，并要求您制作。本研究的大部分结果都依赖于此DNA微阵列芯片，因此我们正在等待这一步的完成。为了验证剪接Valiant拾取系统，我们已经购买了15个人乳腺癌细胞系，并开始分析这些细胞系的激素受体状态，HER 2状态。这项研究的研究预算到2006年就结束了，但我们仍在等待DNA芯片的完成和剪接变异体表达谱的分析，希望能为了解乳腺癌的生物学机制提供有用的结果

A gene arranged on genome creates a functional different gene by alternative splicing. That the ratio of splice valiant changes in estrogen-receptor in breast cancer is reported, and presence of valiant may play important role for carcinoma development. A purpose of this study is to understand expression patterns of Splicing Variant which is associated with outbreak of breast cancer, development, metastasis, drug tolerant acquisition cyclopedically. We would like to establish the gene based treatment system which enables diagnostic treatment of breast cancer by developing of methods to analyze quantitatively in a state of alteration.We listed 128 genes which were reported to contribute or may contribute for breast cancer development. We select 1 to 61 oligos per gene to detect exon-exon junction in the mRNA, and set 1447 oligos in total on DNA microarrays chip. We have planed to use the DNA microarray chip made by the Comb-matrix at first, but we get impossible to use it in a problem of the company's reason, so that we negotiate to be able to use a tip of Gene frontier in a hurry and are asking you for making. Most of result of this study depends on this DNA microarray chip, so we are waiting for completion of this step.To verify the Splicing Valiant pick up system, we have already purchased 15 human breast cancer cell Lines, and have started to analyze hormone receptor states, HER2 states of these cell lines. A research budget for this study becomes the end by 2006, but we wait for completion of DNA chip and analyze the expression profile of Splicing Variant and want to give useful result to understand of biological mechanism of breast cancer