Establishment of breast cancer diagnosis system using alternative splicing detecting DNA microarray

选择性剪接检测DNA微阵列乳腺癌诊断体系的建立

• 批准号: 17591317
• 负责人: SUZUKI Akiko
• 金额: $ 2.37万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591317/
• 关键词: alternative splicing; splice valiant; DNA microarray; breast cancer; 乳がん

A gene arranged on genome creates a functional different gene by alternative splicing. That the ratio of splice valiant changes in estrogen-receptor in breast cancer is reported, and presence of valiant may play important role for carcinoma development. A purpose of this study is to understand expression patterns of Splicing Variant which is associated with outbreak of breast cancer, development, metastasis, drug tolerant acquisition cyclopedically. We would like to establish the gene based treatment system which enables diagnostic treatment of breast cancer by developing of methods to analyze quantitatively in a state of alteration.We listed 128 genes which were reported to contribute or may contribute for breast cancer development. We select 1 to 61 oligos per gene to detect exon-exon junction in the mRNA, and set 1447 oligos in total on DNA microarrays chip. We have planed to use the DNA microarray chip made by the Comb-matrix at first, but we get impossible to use it in a problem of the company's reason, so that we negotiate to be able to use a tip of Gene frontier in a hurry and are asking you for making. Most of result of this study depends on this DNA microarray chip, so we are waiting for completion of this step.To verify the Splicing Valiant pick up system, we have already purchased 15 human breast cancer cell Lines, and have started to analyze hormone receptor states, HER2 states of these cell lines. A research budget for this study becomes the end by 2006, but we wait for completion of DNA chip and analyze the expression profile of Splicing Variant and want to give useful result to understand of biological mechanism of breast cancer

在基因组上排列的基因通过替代剪接产生功能性不同的基因。报道了乳腺癌中雌激素受体的剪接量变化的比例，而英勇的存在可能对癌发育起着重要作用。这项研究的目的是了解与乳腺癌，发育，转移，药物耐受性获得的剪接变体的表达模式。我们想建立基于基因的治疗系统，该系统能够通过开发在变化状态下进行定量分析的方法来诊断乳腺癌。我们列出了128个基因，这些基因据报道有助于或可能有助于乳腺癌的发展。我们选择每个基因1至61个寡核体来检测mRNA中的外显子连接，并在DNA微阵列芯片上总共设定1447个寡聚。我们计划使用Comb-Matrix制作的DNA微阵列芯片，但是我们不可能在公司的理由问题中使用它，以便我们进行谈判，以便能够急忙使用Gene Frontier尖端并要求您进行制作。这项研究的大部分结果都取决于此DNA微阵列芯片，因此我们正在等待完成此步骤。验证剪接勇敢的拾取系统，我们已经购买了15种人类乳腺癌细胞系，并且已经开始分析激素受体状态，这些细胞系的HER2状态。这项研究的研究预算到2006年结束，但是我们等待完成DNA芯片并分析剪接变体的表达概况，并希望给出有用的结果，以了解乳腺癌的生物学机制