A New Methods to Assess Bacterial Translocation

1.大鼠禁食5天后直肠电生理变化及细胞因子变化将大鼠分为禁食组、限食组和对照组。禁食组皮下注射维持液0.1ml/g，连用5天。限制饮食组喂饲0.04kcal/g粉末粉和0.1ml/g水，连续5d。用短路放大器和使用室分析直肠的电生理变化。逆转录-聚合酶链式反应检测直肠壁上皮钠通道、细胞因子(IL-1、6、8、12、干扰素-γ、肿瘤坏死因子α)及其α，β、γ亚基的表达。禁食5天后，直肠电位差和直肠阻力明显降低。给药后，禁食组大鼠的电位和电流变化均显著高于对照组。ENaC的α，β和γ亚基与IL-1、6、12…与对照组相比，禁食组多项指标明显增加。结论：1.直肠的电位和电阻、ENaC的含量和细胞因子的变化反映了直肠黏膜屏障功能的丧失。将6天快速应激大鼠分为4组：1组给予生理盐水1ml/d，2组给予DKT 100 mg/kg/d加入1ml生理盐水中，3组给予DKT 300 mg/kg/d，4组给予DKT 1000 mg/kg/d。进行肠系膜淋巴结培养。RT-PCR法检测肠段细胞因子。测定绒毛数目和绒毛高度。TUNEL法检测细胞凋亡率。培养阳性率第1组为66%，第3组(33%)和第4组(16%)低于第1组，Inf-γ表达第2、3、4组显著低于第1组，IL-6表达第2、3、4组显著低于第1组，绒毛高度和绒毛数目第2、3、4组显著高于第1组，细胞凋亡指数第2、3、4组明显低于第1组，丹参酮通过减轻炎症反应、维持肠道完整性来预防BT。较少

1. Electrophysiological changes and cytokines in the rectum after 5 days fasting rat modelRats were divided into the fast, limited diet and control groups. The fast group received 0.1ml/g with maintenance liquid under the skin for five days. The limited diet group was provided with 0.04kcal/g powder meal and 0.1ml/g water orally for five days. Electrophysiological changes of the rectum were analyzed using a short circuit amplifier and an Using chamber. Epithelial sodium channels (ENaC), the cytokines (IL-1, 6, 8, 12, INF-γ, TNFα) and the α, β and γ subunits of ENaC in the rectal wall were quantified with RT-PCR, respectively. After five days fasting, the electrical potential difference in the rectum and the resistance were significantly decreased. After administration of amirolide, (ENaC blocker), the changes in the electrical potential and current of the fast group were significantly higher than those of the control group, respectively. The α, β and γ subunits of ENaC and IL-1, 6, 12 … More of the fasting group significantly increased compared with those of the control group. The electrical potential and resistance, the quantity of ENaC and the cytokines in the rectum reflected a failure of mucosal barrier function.2. Preventive effects of Kampo medicine "Dai-kenchu-to" (DKT) against BTRats, subjected to fast stress for 6 days, were divided into the four groups : Group 1 receiving only 1ml/day physiologic saline; Group 2 receiving DKT (100mg/kg/day) in 1ml physiologic saline ; Group 3 and Group 4 receiving 300mg and 1000mg/kg/day of DKT, respectively. The mesenteric lymph node culture was performed. The cytokines of intestinal segments were assessed using RT-PCR. The number of villus and villous height was assessed. Apoptosis was measured by TUNEL staining. Positive rate in culture was 66% in Group 1. The rate in Group 3 (33%) and Group 4 (16%) was lower than in Group 1. INF-γ expression in Groups 2,3 and 4 was significantly lower than in Group 1. Interleukin-6 expression in Groups 2 and 3 was significantly lower than in Group 1. Villous height and number of villus in Group 2, 3 and 4 was significantly taller and greater than in Group 1. Apoptotic index in Group 2,3 and 4 was significantly lower than in Group 1. DKT prevents BT by reducing inflammatory reaction and maintaining intestinal integrity. Less