Development of Diagnostic Method for clinical utility on Thyroid Carcinoma by Chemical assay

胸腺球蛋白（Tg）由甲状腺产生，分子量为66万道尔顿，碳水化合物含量约为10%。传统上，通过测定血清胸腺球蛋白来随访分化型甲状腺癌。然而，免疫测定法测得的胸腺球蛋白浓度受到血清中抗tg自身抗体存在的极大影响。我们开发了一种新的诊断甲状腺癌的方法——LCA凝集素化学检测（LCA反应Tg法）。本研究对103例健康志愿者、159例甲状腺良性肿瘤患者、170例甲状腺癌患者的血清样本进行了研究。甲状腺癌患者的LCA反应性Tg值明显低于甲状腺良性肿瘤患者的血清Tg值。血清LCA反应性Tg测定值可用于甲状腺癌与良性甲状腺肿瘤的鉴别。

Thymoglobulin (Tg) is produced by the thyroid with a molecular mass of 660,000 Dalton and a carbohydrate content of approximately 10%. Traditionally, the follow-up of differentiated thyroid carcinoma by measured serum Thymoglobulin. However, the concentration of thymoglobulin measured by immunoassay is greatly affected by presence of anti-Tg autoantiboies in sera.We developed of new diagnostic method for clinical utility on thyroid carcinoma by chemical assay using LCA lectin(LCA reactive Tg method). The study was conducted on 103 serum samples form healthily volunteers, 159 patients with benign thyroid tumor, 170 patients with thyroid carcinoma.The values of LCA reactive Tg method in patients with thyroid carcinoma was significantly lower than in patients those with benign thyroid tumor with serum Tg. The values of LCA reactive Tg method in serum may be useful for distinguishing between thyroid carcinoma and benign thyroid tumor.