Development of Diagnostic Method for clinical utility on Thyroid Carcinoma by Chemical assay

甲状腺癌化学检测临床诊断方法的发展

• 批准号: 17591343
• 负责人: KATO Ryoji
• 金额: $ 2.05万
• 依托单位: Kagawa prefectural College of Health Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591343/
• 关键词: Thyroglobulin; Thyroid cancer; Lectin; Monoclonal antibody; Sugar chain; サイクログロブリン; 甲状腺濾胞がん; 診断法

Thymoglobulin (Tg) is produced by the thyroid with a molecular mass of 660,000 Dalton and a carbohydrate content of approximately 10%. Traditionally, the follow-up of differentiated thyroid carcinoma by measured serum Thymoglobulin. However, the concentration of thymoglobulin measured by immunoassay is greatly affected by presence of anti-Tg autoantiboies in sera.We developed of new diagnostic method for clinical utility on thyroid carcinoma by chemical assay using LCA lectin(LCA reactive Tg method). The study was conducted on 103 serum samples form healthily volunteers, 159 patients with benign thyroid tumor, 170 patients with thyroid carcinoma.The values of LCA reactive Tg method in patients with thyroid carcinoma was significantly lower than in patients those with benign thyroid tumor with serum Tg. The values of LCA reactive Tg method in serum may be useful for distinguishing between thyroid carcinoma and benign thyroid tumor.

胸腺球蛋白（Tg）由甲状腺产生，分子量为 660,000 道尔顿，碳水化合物含量约为 10%。传统上，分化型甲状腺癌的随访是通过测量血清胸腺球蛋白来进行的。然而，通过免疫测定法测得的胸腺球蛋白浓度受血清中抗Tg自身抗体存在的影响很大。我们通过使用LCA凝集素进行化学测定（LCA反应性Tg法），开发了临床实用的甲状腺癌新诊断方法。本研究对103名健康志愿者、159名甲状腺良性肿瘤患者、170名甲状腺癌患者的血清样本进行了研究。甲状腺癌患者的LCA反应性Tg法测定值显着低于甲状腺良性肿瘤患者的血清Tg值。血清中LCA反应性Tg法的值可能有助于区分甲状腺癌和良性甲状腺肿瘤。

项目成果

The Clinical Utility of Lens culinaris Agglutinin-Reactive Thyroglobulin Ratio in Serum for Distinguishing Benign from Mlignant Codition of Thyroid

血清中菜豆凝集素反应性甲状腺球蛋白比值鉴别甲状腺良恶性疾病的临床应用

• 发表时间: 2007
• 期刊: Clinica Chimica Acta 379
• 作者: Shimizu K;kato R;et al.
• 通讯作者: et al.

抗サイログロブリン自己抗体が認識するTg抗原エピトープの研究.

抗甲状腺球蛋白自身抗体识别的 Tg 抗原表位的研究。

• 发表时间: 2006
• 期刊: 医学検査 55巻(3)
• 作者: 亀子文子;天川雅夫;加藤亮二
• 通讯作者: 加藤亮二

Disrimination of Thyroglobulin form Thyroid Carcinoma Tissue and from benign thyroid Tissues with Use of Competitive Assay between Lectin and Anti-Thyroglobulin Antibody

使用凝集素和抗甲状腺球蛋白抗体之间的竞争性测定区分甲状腺球蛋白来自甲状腺癌组织和良性甲状腺组织

• 发表时间: 2007
• 期刊: 臨床病理 54(8)(印刷中)
• 作者: Shimizu K;kato R;et al.
• 通讯作者: et al.

The Clinical Utility of Lens culinaris Agglutinin-Reactive Thymoglobulin Ratio in Serum for Distinguishing Benign from Malignant Condition of Thyroid

血清中菜豆凝集素反应性胸腺球蛋白比值鉴别甲状腺良恶性的临床应用

• 发表时间: 2007
• 期刊: Clinica Chimica Acta 379
• 作者: Shimizu;K.Kato;R et al.
• 通讯作者: R et al.

Discrimination of Thymoglobulin form Thyroid Carcinoma Tissue and from benign thyroid Tissues with Use of Competitive Assay between Lectin and Anti-Thymoglobulin Antibody

使用凝集素和抗胸腺球蛋白抗体之间的竞争测定法区分甲状腺癌组织和良性甲状腺组织中的胸腺球蛋白

• 发表时间: 2007
• 期刊: Clin.Pathology 54
• 作者: Shimizu;K.Kato;R et al.
• 通讯作者: R et al.