Development of a New Diagnostic Method for Thyroid Carcinoma by Measurement of Lectin Inhibitory Thyroglobulin

通过测量凝集素抑制性甲状腺球蛋白开发甲状腺癌新诊断方法

• 批准号: 15591370
• 负责人: KATO Ryoji
• 金额: $ 1.66万
• 依托单位: Kagawa Prefctural College of Health Sciences (2004)Kagawa Prefectural College of Health science (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591370/
• 关键词: Thyroid Cancer; microheterogeneity; lectin; Thyroglobulin; 血清; 穿刺液; モノクローナル抗体; 甲状腺癌診断; 診断法

Thyloglobulin (Tg) is produced by the thyroid with a molecular mass of 660,000 Dalton and a carbohydrate content of approximately 10%. Lectin affinity binding profile of Tg was carried out on samples obtained from thyroid tissue and serum from patients with benign and malignant diseases with the use of Concanavalin A, Lens culinaris agglutinin (LCA), Da tura stramonium agglutinin, and Ricin us communis agglutinin-120. Difference in percentage of Lens culinaris agglutinin (LCA) reactive Tg to total Tg was detected in thyroid tissue extract, as well as in serum from patients with the benign and malignant conditions, however, no significant differences were found with other lectins. The percentage of LCA reactive Tg in tissue and serum had a tendency to decrease in the malignant cases, particularly in patients with thyroid cancer having the lymph node metastasis in that significantly highly percentage ratio of LCA-reactive Tg had been demonstrated. This is the first study to show the differences in microheterogeneity of Tg, with respect to its sugar chain structure in clinically confirmed thyroid tumors in an attempt to identifying thyroid malignancies. The differences LCA profiling of Tg indicate its potential important clinical utility in discriminating of malignant conditions.

甲状腺球蛋白（Tg）由甲状腺产生，分子量为 660,000 道尔顿，碳水化合物含量约为 10%。使用刀豆球蛋白 A、小扁豆凝集素 (LCA)、Da tura stramonia 凝集素和蓖麻毒素凝集素-120，对从良性和恶性疾病患者的甲状腺组织和血清中获得的样品进行 Tg 的凝集素亲和结合谱分析。在甲状腺组织提取物以及良性和恶性疾病患者的血清中检测到小扁豆凝集素 (LCA) 反应性 Tg 占总 Tg 的百分比存在差异，但与其他凝集素没有发现显着差异。在恶性病例中，组织和血清中LCA反应性Tg的百分比有降低的趋势，特别是在有淋巴结转移的甲状腺癌患者中，LCA反应性Tg的百分比显着升高。这是第一项研究，显示临床证实的甲状腺肿瘤中 Tg 的糖链结构的微观异质性差异，以试图识别甲状腺恶性肿瘤。 Tg 的 LCA 分析差异表明其在区分恶性疾病方面具有潜在的重要临床用途。

项目成果

Detection of altered glycosylation pattern of Thyroglobulin in thyroid Malignancy

甲状腺恶性肿瘤中甲状腺球蛋白糖基化模式改变的检测

• 发表时间: 2005
• 期刊: Clinical Endocrinology 53
• 作者: Kato R;et al.
• 通讯作者: et al.

糖鎖変異検出による甲状腺癌診断法

检测糖链突变的甲状腺癌诊断方法

• 发表时间: 2005
• 期刊: ホルモンと臨床(分子甲状腺学の進歩2005) 53巻1号
• 作者: Kato R;et al.;加藤亮二他
• 通讯作者: 加藤亮二他

甲状腺糖鎖変異による甲状腺癌診断法

基于甲状腺糖链突变的甲状腺癌诊断方法

• 发表时间: 2005
• 期刊: ホルモンと臨床〔分子甲状腺学の進歩2005〕 53巻
• 作者: 加藤亮二 ほか

加藤亮二: "レクチン阻害サイログロブリン測定による新しい甲状腺乳頭癌診断法の開発"香川県立医療短大紀要. 5巻. 39-44 (2004)

加藤亮司：“通过测量凝集素抑制的甲状腺球蛋白开发乳头状甲状腺癌的新诊断方法”香川县立医学院通报第 5 卷 39-44（2004 年）。