Development of a New Diagnostic Method for Thyroid Carcinoma by Measurement of Lectin Inhibitory Thyroglobulin

甲状腺球蛋白（Tg）由甲状腺产生，分子量为660，000道尔顿，碳水化合物含量约为10%。使用伴刀豆球蛋白A、透镜culinaris凝集素（LCA）、曼陀罗凝集素和蓖麻凝集素-120，对从良性和恶性疾病患者的甲状腺组织和血清获得的样品进行Tg的凝集素亲和结合谱。在甲状腺组织提取物以及良性和恶性疾病患者的血清中，检测到透镜凝集素（LCA）反应性Tg占总Tg的百分比存在差异，但与其他凝集素无显著差异。在恶性病例中，组织和血清中LCA反应性Tg的百分比有下降的趋势，特别是在具有淋巴结转移的甲状腺癌患者中，LCA反应性Tg的百分比显著升高。这是第一项研究，以显示Tg的微观异质性的差异，就其糖链结构在临床确诊的甲状腺肿瘤，试图确定甲状腺恶性肿瘤。Tg的LCA谱的差异表明其在鉴别恶性疾病中的潜在重要临床实用性。

Thyloglobulin (Tg) is produced by the thyroid with a molecular mass of 660,000 Dalton and a carbohydrate content of approximately 10%. Lectin affinity binding profile of Tg was carried out on samples obtained from thyroid tissue and serum from patients with benign and malignant diseases with the use of Concanavalin A, Lens culinaris agglutinin (LCA), Da tura stramonium agglutinin, and Ricin us communis agglutinin-120. Difference in percentage of Lens culinaris agglutinin (LCA) reactive Tg to total Tg was detected in thyroid tissue extract, as well as in serum from patients with the benign and malignant conditions, however, no significant differences were found with other lectins. The percentage of LCA reactive Tg in tissue and serum had a tendency to decrease in the malignant cases, particularly in patients with thyroid cancer having the lymph node metastasis in that significantly highly percentage ratio of LCA-reactive Tg had been demonstrated. This is the first study to show the differences in microheterogeneity of Tg, with respect to its sugar chain structure in clinically confirmed thyroid tumors in an attempt to identifying thyroid malignancies. The differences LCA profiling of Tg indicate its potential important clinical utility in discriminating of malignant conditions.