Development of Detection System for Thyroid Carcinoma Specific Antigen and its Function Analysis
甲状腺癌特异性抗原检测系统的研制及其功能分析
基本信息
- 批准号:13671270
- 负责人:
- 金额:$ 1.47万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The aim of this study was to differentiate thyroid papillary carcinoma from the other. We first developed monoclonal antibodies that could specifically recognize a thyroid tumor-associated antigen, which enabled us to investigate the expression of this antigen in various tissues by western blotting and ELISA. We developed two types of monoclonal antibodies such as thyroid carcinoma antibodies (CAb) and thyroglobulin (Tg) antibodies (TgAb). Using the TgAb, an ELISA system was established to differentiate thyroid cancer tissues from normal thyroid tissue, based on the previous observation that there are differences in the reactivity with lectins between the sugar chains in thyroid cancer associated Tg and normal Tg. In western blotting using CAb, a protein of about 40kDa was observed in all the papillary carcinoma associated tissue specimens. In contrast, the 40kDa band was never detected in any of the other thyroid tissues. Subsequently, the ratios of unbound serum Tg {Tg-unbound (%)} in various thyroid diseases were investigated by ELISA using LCA lectin. The Tg-unbound (%) in papillary carcinoma was significantly higher than that observed in benign goiter and in normal controls. These results clearly indicate that the monoclonal antibody-based western blotting and lectin-based ELISA would significantly contribute to diagnose patients with thyroid papillary carcinoma. Monoclonal antibodies specific for the 40 kDa protein is under development.
本研究的目的是将甲状腺乳头状癌与其他甲状腺乳头状癌区别开来。我们首次开发了能够特异性识别甲状腺肿瘤相关抗原的单抗,这使得我们能够通过Western blotting和ELISA法研究这种抗原在各种组织中的表达。我们研制了两种类型的单抗:甲状腺癌抗体(CAb)和甲状腺球蛋白抗体(TgAb)。根据甲状腺癌相关甘油三酯和正常甘油三酯糖链与凝集素反应性的差异,建立了一种区分甲状腺癌组织和正常甲状腺组织的酶联免疫吸附试验体系。在CAB蛋白免疫印迹中,所有乳头状癌相关组织标本中均检测到约40 kDa的蛋白。相比之下,在其他任何甲状腺组织中都没有检测到40 kDa的条带。随后,用LCA凝集素酶联免疫吸附试验检测不同甲状腺疾病患者血清游离甘油三酯(TG)的比例。乳头状癌的甘油三酯游离(%)显著高于良性甲状腺肿组和正常对照组。这些结果清楚地表明,以单抗为基础的Western blotting和以凝集素为基础的ELISA法对甲状腺乳头状癌的诊断有重要意义。针对40 kDa蛋白的单抗正在开发中。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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KATO Ryoji其他文献
KATO Ryoji的其他文献
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{{ truncateString('KATO Ryoji', 18)}}的其他基金
Development of Diagnostic Method for clinical utility on Thyroid Carcinoma by Chemical assay
甲状腺癌化学检测临床诊断方法的发展
- 批准号:
17591343 - 财政年份:2005
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of a New Diagnostic Method for Thyroid Carcinoma by Measurement of Lectin Inhibitory Thyroglobulin
通过测量凝集素抑制性甲状腺球蛋白开发甲状腺癌新诊断方法
- 批准号:
15591370 - 财政年份:2003
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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