Development of Detection System for Thyroid Carcinoma Specific Antigen and its Function Analysis

甲状腺癌特异性抗原检测系统的研制及其功能分析

• 批准号: 13671270
• 负责人: KATO Ryoji
• 金额: $ 1.47万
• 依托单位: Kagawa Prefectural College of Health Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671270/
• 关键词: Thyroid Cancer; Monoclonal antibody; Sugar chain; Lectin; ELISA; Western blotting; 糖類変異

The aim of this study was to differentiate thyroid papillary carcinoma from the other. We first developed monoclonal antibodies that could specifically recognize a thyroid tumor-associated antigen, which enabled us to investigate the expression of this antigen in various tissues by western blotting and ELISA. We developed two types of monoclonal antibodies such as thyroid carcinoma antibodies (CAb) and thyroglobulin (Tg) antibodies (TgAb). Using the TgAb, an ELISA system was established to differentiate thyroid cancer tissues from normal thyroid tissue, based on the previous observation that there are differences in the reactivity with lectins between the sugar chains in thyroid cancer associated Tg and normal Tg. In western blotting using CAb, a protein of about 40kDa was observed in all the papillary carcinoma associated tissue specimens. In contrast, the 40kDa band was never detected in any of the other thyroid tissues. Subsequently, the ratios of unbound serum Tg {Tg-unbound (%)} in various thyroid diseases were investigated by ELISA using LCA lectin. The Tg-unbound (%) in papillary carcinoma was significantly higher than that observed in benign goiter and in normal controls. These results clearly indicate that the monoclonal antibody-based western blotting and lectin-based ELISA would significantly contribute to diagnose patients with thyroid papillary carcinoma. Monoclonal antibodies specific for the 40 kDa protein is under development.

这项研究的目的是将甲状腺乳头状癌与另一个区分开。我们首先开发了单克隆抗体，可以特异性地识别甲状腺肿瘤相关的抗原，这使我们能够通过Western blotting和Elisa研究该抗原在各种组织中的表达。我们开发了两种类型的单克隆抗体，例如甲状腺癌抗体（CAB）和甲状腺球蛋白（TG）抗体（TGAB）。使用TGAB，基于先前的观察结果，即甲状腺癌链与甲状腺癌链之间与甲状腺相关的TG和正常TG之间的反应性差异，建立了ELISA系统，以将甲状腺癌组织与正常甲状腺组织区分开。在使用CAB的蛋白质印迹中，在所有相关的乳头癌的组织样品中都观察到约40kDa的蛋白质。相反，在其他任何甲状腺组织中从未检测到40KDA条带。随后，使用LCA凝集素研究了各种甲状腺疾病中未结合的血清Tg {Tg-unbound（％）}的比率。乳头状癌中的TG突出（％）明显高于良性甲状腺肿和正常对照中观察到的TG（％）。这些结果清楚地表明，基于单克隆抗体的蛋白质印迹和基于凝集素的ELISA将显着有助于诊断甲状腺乳头状癌患者。针对40 kDa蛋白的单克隆抗体正在开发中。