Detection and clinical application of Stem Cell Factor in Squamous cell tissue and cancer.

我：Sox-2作为干细胞因子在鳞状细胞癌细胞中的致癌反式激活靶基因的寻找和鉴定：我们用Affirons微阵列芯片比较了293细胞中Sox-2表达和不表达的总RNA，获得了表达谱。在该表达谱中，我们发现了两个被Sox-2表达反式激活的候选癌基因和两个被其表达反式抑制的候选抑癌基因。II.检测调控Sox-2表达的基因：我们克隆了Sox-2基因启动子区约500 bp的片段。我们将该片段亚克隆到pGL 3载体中以分析启动子。因此，我们将能够检测转录激活剂或抑制剂的Sox-2基因的表达的分析。III：Sox-2基因作为鳞状细胞癌的干细胞因子的临床应用：我们研究了其有用的可能性作为鳞状细胞癌的肿瘤标志物，通过定量检测Sox-2 mRNA在围手术期外周血。Sox-2基因的扩增：Sox-2基因位于3q 26 -27，在各种癌组织中均有扩增。我们采用Southern印迹技术检测了60例食管癌中12例的基因组扩增。提示Sox-2基因是鳞状细胞癌的一个癌基因。

I : Search and identification of the oncogenic transactivated target genes by Sox-2 as the Stem cell factor in squamous cancer cells : We compared the total RNA with or without Sox-2 expression in 293 cells using Affimetrix micro-array chip to obtain the profile. In the profile, we found two candidate oncogenes transactivated by Sox-2 expression, and two tumor suppressor genes transrepressed by its expression.II : Detecting genes which regulate Sox-2 expression: We cloned an about 500 bp fragment of the promoter region of Sox-2 gene. We sub-cloned the fragment into the pGL3 vector to analyze a promoter. We thus will be able to detect transcription activators or repressors of Sox-2 gene expression by the analysis.III : Clinical application of Sox-2 gene as the Stem cell factor for squamous cell cancer : We studied the possibilities of its usefulness as a tumor marker for squamous cell cancer by quantitative detection of Sox-2 mRNA in perioperative peripheral blood. Sox-2 mRNA which was detectable in the peripheral blood before operation, could no longer be detected at 14 postoperative days in 5 of 8 esophageal cancer patients.IV : Gene amplification of Sox-2 gene: Sox-2 gene locates at 3q26-27, where genome amplification is detected in various cancer tissues. We detected genome amplification in 12 of 60 cases in esophageal cancer by Southern blot technique. The finding suggests that Sox-2 gene is an oncogene for the squamous cell cancer.