课题基金 / 基金详情

Detection and clinical application of Stem Cell Factor in Squamous cell tissue and cancer.

Detection and clinical application of Stem Cell Factor in Squamous cell tissue and cancer.
鳞状细胞组织和癌症中干细胞因子的检测及临床应用。
批准号:
17591391
负责人:
NOMOTO Shuji
金额:
$2.24万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2005
资助国家:
日本
项目状态:
已结题
起止时间:
2005 至 2006

项目摘要

项目成果

NOMOTO Shuji的其他基金

相似基金

相关文献

中文摘要
翻译
SOX-2作为鳞癌细胞干细胞因子的致癌反式激活靶基因的寻找和鉴定:我们利用Affimetrix微阵列芯片比较了293细胞中SOX-2表达前后的总RNA,以获得该基因的表达谱。在这个图谱中,我们发现了两个受SOX-2表达反式激活的候选癌基因,以及两个受其表达反式抑制的肿瘤抑制基因。II:检测调控SOX-2表达的基因:我们克隆了SOX-2基因启动子区域约500bp的片段。我们将该片段亚克隆到pGL3载体中,对启动子进行分析。因此,我们将能够通过分析来检测SOX-2基因表达的转录激活或抑制因子。III:SOX-2基因作为鳞状细胞癌干细胞因子的临床应用:我们通过定量检测围手术期外周血中SOX-2基因的mRNA,研究了其作为鳞癌肿瘤标志物的可能性。8例食道癌患者手术前外周血中检测到SOX-2基因,术后14天有5例患者检测不到SOX-2基因。IV:SOX-2基因扩增:SOX-2基因位于3q26-27,在多种癌组织中均可检测到基因组扩增。应用Southern杂交技术对60例食管癌组织中的12例进行了基因组扩增。这一发现表明SOX-2基因是鳞状细胞癌的癌基因。
英文摘要
I : Search and identification of the oncogenic transactivated target genes by Sox-2 as the Stem cell factor in squamous cancer cells : We compared the total RNA with or without Sox-2 expression in 293 cells using Affimetrix micro-array chip to obtain the profile. In the profile, we found two candidate oncogenes transactivated by Sox-2 expression, and two tumor suppressor genes transrepressed by its expression.II : Detecting genes which regulate Sox-2 expression: We cloned an about 500 bp fragment of the promoter region of Sox-2 gene. We sub-cloned the fragment into the pGL3 vector to analyze a promoter. We thus will be able to detect transcription activators or repressors of Sox-2 gene expression by the analysis.III : Clinical application of Sox-2 gene as the Stem cell factor for squamous cell cancer : We studied the possibilities of its usefulness as a tumor marker for squamous cell cancer by quantitative detection of Sox-2 mRNA in perioperative peripheral blood. Sox-2 mRNA which was detectable in the peripheral blood before operation, could no longer be detected at 14 postoperative days in 5 of 8 esophageal cancer patients.IV : Gene amplification of Sox-2 gene: Sox-2 gene locates at 3q26-27, where genome amplification is detected in various cancer tissues. We detected genome amplification in 12 of 60 cases in esophageal cancer by Southern blot technique. The finding suggests that Sox-2 gene is an oncogene for the squamous cell cancer.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Detection of novel cancer-related genes by using triple combination array analysis
  • 批准号:
    22591427
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $3.0万
  • 财政年份:
    2010
  • 负责人:
    NOMOTO Shuji
  • 依托单位:
Influence of mutation type of p53-FGF-1 pathway in angiogenesis for cancerous tissue and metastasis
  • 批准号:
    19590386
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $2.91万
  • 财政年份:
    2007
  • 负责人:
    NOMOTO Shuji
  • 依托单位:
海外基金