The effects of anesthetics on aquaporin-5

麻醉药对水通道蛋白5的影响

• 批准号: 17592087
• 负责人: NAKAJO Nobuyoshi
• 金额: $ 1.92万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592087/
• 关键词: Aquaporin; Anesthetics; Salivation

We examined the effects of anesthetic agent for the function of aquaporin-5 (AQP5) by Xenopus expression system. Oocytes from Xenopus Laevis microinjected with in vitro-transcripted AQP5 mRNA exhibited increased these volume; this was reversibly inhibited by mercuric chloride, a known inhibitor of water channels. The rate of volume change due to osmotic effects was imaged on the camera connected to an area analyzer. Serial images were stored at 1-sec intervals in a computer. We compared actions of different anesthetic drugs, such as pentobarbital (50-1000 uM), propofol(1-200 uM), ketamine (1-100 uM), midazolam (1-200 uM), sevoflurane (1 mM) and isoflurane (1 mM), lidocaine (1 uM), benzocaine (1 uM) and QX-314 (1 uM). The ratio of volume change was inhibited by pentobarbital and propofol while ketamine and midazolam had no effect. On the other hand, sevoflurane and isoflurane increased the ratio of volume change by AQP5. Lidocaine, benzocaine (permanently uncharged) and QX-314 (permanently charged) had no effects for the ratio of volume change. These results suggest that some anesthetics may indeed affect the function of AQP5.

本研究利用非洲爪蟾表达系统研究了麻醉剂对水通道蛋白5（AQP 5）功能的影响。非洲爪蟾卵母细胞显微注射在体外转录的AQP 5 mRNA表现出增加这些体积，这是可逆的抑制氯化汞，一种已知的水通道抑制剂。由于渗透效应引起的体积变化率在连接到面积分析仪的照相机上成像。连续图像以1秒的间隔存储在计算机中。我们比较了不同麻醉药物的作用，如戊巴比妥（50-1000 uM）、丙泊酚（1-200 uM）、氯胺酮（1-100 uM）、咪达唑仑（1-200 uM）、七氟烷（1 mM）和异氟烷（1 mM）、利多卡因（1 uM）、苯并咪唑啉（1 uM）和QX-314（1 uM）。戊巴比妥和异丙酚对容积变化率有抑制作用，氯胺酮和咪达唑仑对容积变化率无影响。另一方面，七氟醚和异氟醚增加了AQP 5的体积变化率。利多卡因、苯并咪唑（永久不带电）和QX-314（永久带电）对容积变化率无影响。这些结果表明，一些麻醉剂可能确实影响AQP 5的功能。