Relationship between neuropeptide and bone remodeling during tooth movement.

牙齿移动过程中神经肽与骨重塑的关系。

• 批准号: 17592138
• 负责人: DEGUCHI Toru
• 金额: $ 2.05万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592138/
• 关键词: tooth movement; neuropeptide; bone remodeling; trigeminal ganglion; ノックアウトマウス

The purpose of this study was to investigate the role of neuropeptide (galanin) in bone remodeling during tooth movement. In the past, we reported a significant increase of the expression of galanin in periodontal ligament during tooth movement. We analyzed the expression of galanin positive cell in the trigeminal ganglion during tooth movement. After the initiation of tooth movement in rats, the number of galanin positive cells and size were quantitatively analyzed. Furthermore, we performed a double stain with galanin and calcitonin gene-related peptide and analyzed the rate of the coexistence. As a result, in trigeminal ganglion, galanin positive cell were remarkably increased after tooth movement. Significant change in the size of galanin-positive cells was also observed. Furthermore, the coexistence rate of galanin and calcitonin gene-related peptide was increased after tooth movement. Therefore, the origin of the increased galanin in the periodontal ligament is suggested to be the trigeminal ganglion that plays an important role on transmission of pain during tooth movement. These results were published in Journal of Dental Research. The murine bone marrow culture was stimulated by M-CSF and RANKL to form osteoclast formation in the presence or absence of galanin. TRAP positive multinucleated cells including 3 or more nuclei were defined as osteoclasts. mRNA expression was detected by RT-PCR. Galanin significantly inhibited osteoclast formation in murine bone marrow culture in a dose-dependent fashion. We also found that 3 distinct subtypes mRNA of galanin receptor (type 1-type 3) was expressed in murine bone marrow cells. Therefore, It is possible that galanin plays a critical role in regulating osteoclastic bone remodeling by neural systems. This result is in preparation to be submitted in the Journal.

本研究的目的是探讨神经肽(甘丙肽)在牙齿移动过程中骨改建中的作用。过去，我们曾报道在牙齿移动过程中，甘丙素在牙周膜中的表达显著增加。我们分析了牙齿移动过程中三叉神经节甘丙素阳性细胞的表达。在大鼠牙齿移动开始后，对甘丙素阳性细胞的数量和大小进行定量分析。此外，我们还进行了甘丙肽和降钙素基因相关肽的双重染色，并分析了共存的比率。结果表明，牙齿移动后，三叉神经节内甘丙素阳性细胞明显增多。甘丙肽阳性细胞的大小也发生了显著变化。此外，移动牙齿后甘丙素和降钙素基因相关肽的共存率增加。因此，牙周膜甘丙素升高的来源可能是三叉神经节，三叉神经节在牙齿移动过程中起着传递疼痛的重要作用。这些研究结果发表在《牙科研究杂志》上。用M-CSF和RANKL刺激小鼠骨髓细胞在甘丙素存在或不存在的情况下形成破骨细胞。TRAP阳性的多核细胞包括3个或3个以上的核，定义为破骨细胞。逆转录-聚合酶链式反应(RT-PCR)检测mRNA表达。甘丙素以剂量依赖的方式显著抑制小鼠骨髓培养破骨细胞的形成。我们还发现在小鼠骨髓细胞中有3种不同亚型的甘丙肽受体(1型-3型)表达。因此，甘丙素可能在神经系统调节破骨细胞性骨重建中起关键作用。这一结果正在准备中，将在《华尔街日报》上提交。

项目成果

Histomorphometric evaluation of alveolar bone turnover between the maxilla and mandible during experimental tooth movement in dogs

狗实验性牙齿移动期间上颌骨和下颌骨之间牙槽骨转换的组织形态学评估

• 发表时间: 2007
• 期刊: Am J Orthod Dentofac Orthop (in press)
• 作者: Sato J;et al.;Deguchi T et al.;Miyuki Yamaura;Giovanna Kishimoto;Miyawaki S.;Isis Barros;Deguchi T et al.
• 通讯作者: Deguchi T et al.

Treatment of severe anterior open bite with skeletal anchorage in adult ; A comparison with orthognathic surgery outcomes

成人严重前牙开颌骨支抗治疗；

• 发表时间: 2006
• 期刊: Am J Orthod. Dentofac Orthop (in press)
• 作者: Obata;K. et al.;Kuroda S;Deguchi T et al.;Kuroda S et al.;Kuroda S;Kuroda S et al.
• 通讯作者: Kuroda S et al.

歯界展望特別号2005 インプラント矯正-その有用性と今後の展望

Dental Perspectives 2005 年特刊 种植体正畸学 - 其实用性和未来前景

• 发表时间: 2005
• 作者: 平下斐雄;山本 照子;山本 照子 他
• 通讯作者: 山本 照子 他

Root proximity is a major factor for screw failure in orthodontic anchorage

• DOI: 10.1016/j.ajodo.2006.06.017
• 发表时间: 2007-04-01
• 期刊: AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS
• 影响因子: 3
• 作者: Kuroda, Shingo;Yamada, Kazuyo;Takano-Yamamoto, Teruko
• 通讯作者: Takano-Yamamoto, Teruko

Quantitative evaluation of cortical bone thickness using CT scanning for implant-orthodontics

使用 CT 扫描定量评估种植正畸中的皮质骨厚度

• 发表时间: 2006
• 期刊: Am J Orthod Dentofac Orthop 129
• 作者: Mizushima;T. et al.;Deguchi T et al.
• 通讯作者: Deguchi T et al.