Development of a new periodontal regeneration technique for the teeth with reduced periodontal ligament using the combination of tissue culture and transplantation of the teeth

有研究表明，来自牙周韧带的细胞有可能产生新的结缔组织附着。体内由于上皮向下生长，牙周膜细胞在创面根表面的聚集有限。我们研究了一种采用组织培养与移植相结合的牙周膜缺损牙的牙周再生技术。本研究旨在探讨牙釉质基质衍生物（EMD）对牙周韧带细胞体外移植后牙周愈合的影响。拔除小猎犬门牙后，拔除牙周韧带和牙骨质距牙根冠状部分5mm处，刨平牙根表面。牙根尖部的牙周韧带保留。在根平面表面用24% EDTA蚀刻后，在根平面表面涂上Emdogain。在含抗体和15%胎牛血清的a-MEM中培养6周后移植。移植后8周，制备标本进行组织学分析。Emdogain应用组根平面多见新牙骨质及纤维形成。由此可见，牙釉质基质衍生物能增加体外培养牙移植后alp阳性细胞的面积，促进牙骨质的形成。

It has been suggested that cells from the periodontal ligament have the potential to create new connective tissue attachment. The periodontal ligament cells populated limitedly on the root surface of the wound in vivo due to epithelium down growth. We have been developing a new periodontal regeneration technique for the teeth with reduced periodontal ligament using the combination of tissue culture and transplantation of the teeth.The purpose of this study was to examine effects of enamel matrix derivative (EMD) application on periodontal healing after implantation of teeth with cells populating from periodontal ligament in vitro. After the Incisors from beagles were extracted, the periodontal ligament and cementum at 5 mm from coronal part of the roots were removed and the root surfaces were planed. The periodontal ligament in the apical part of the root was retained. Following the etching by 24 % EDTA on the root planed surface, Emdogain was applied on the root planed surface. After the teeth were incubated in a-MEM with antibodies and 15% fetal bovine serum for 6 weeks, the teeth were transplanted. Eight weeks after transplantation, the specimens were prepared for histological analysis.Most of the root planed surfaces of Emdogain application group were observed new cementum with fibers. It was concluded the enamel matrix derivative could increase the area of ALP-positive cells and enhance cementum formation after transplantation of teeth cultured in vitro.