Binding Affinity of Actinobacillus actinomycetemcomitans for extracellular matrix proteins

伴放线放线杆菌对细胞外基质蛋白的结合亲和力

基本信息

  • 批准号:
    17592190
  • 负责人:
  • 金额:
    $ 1.28万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2005
  • 资助国家:
    日本
  • 起止时间:
    2005 至 2006
  • 项目状态:
    已结题

项目摘要

Actinobacillus actinomycetemcomitans is an aetiologic agent in the development of periodontal and some systemic diseases in humans. In this study, the binding affinity of A. actinomycetemcomitans to 4 kinds of extracellular matrix protein (collagen type I, collagen type IV, fibronectin, and laminin) was investigated. A. actinomycetemcomitansY4 and IDH781 bound to immobilized fibronectin and collagen type IV more than collagen type I and laminin. The binding of the cells to immobilized fibronectin and type IV collagen was inhibited by both of soluble fibronectin and collagen type N. The soluble fibronectin, but not the soluble collagen type IV bound to the 40-kDa proteins that were extracted from Y4 and IDH781 cells. These results suggest that Actinobacillus actinomycetemcomitansY4 and IDH781 have high binding affinity for fibronectin and this binding may be regulated with the 40-kDa bacterial cell proteins. Y4 and IDH781 also showed high affinity for collagen type IV and the non-protein component of bacterial cells may be involved in binding to collagen type IV.The cell surface protein antigen (PAg) and glucosyltransferases (GTFs) produced by Streptococcus sobrinus are major colonization factors of the organism, and the inhibition of these two factors should provide protection against dental caries. In this study, we constructed a fusion protein (PAgA-GB) composed of the alanine-rich region of PAg and the glucan-binding domain of GTF, and the antibodies against the fusion protein were prepared in rabbits. The antibody against PAgA-GB significantly inhibited the sucrose-dependent adhesion, but not sucrose-independent adhesion. These results show that the antibody against GTF-I may be useful for effective inhibition of the sucrose-dependent adhesion of S. sobrinus. However, PAg of S sobrinus may not function primarily as a receptor for acquired pellicles, and other cell surface proteins may be involved in the sucrose-independent adhesion of S sobrinus.
伴放线放线杆菌是引起人类牙周及某些系统性疾病的病原菌。在本研究中,A.研究了放线菌共生菌对4种细胞外基质蛋白(I型胶原、IV型胶原、纤连蛋白和层粘连蛋白)的作用。A.放线菌共生菌Y 4和IDH 781与固定化纤连蛋白和IV型胶原的结合能力强于I型胶原和层粘连蛋白。可溶性纤连蛋白和N型胶原蛋白均可抑制细胞与固定化纤连蛋白和IV型胶原的结合。可溶性纤连蛋白,但不是可溶性IV型胶原结合到从Y 4和IDH 781细胞提取的40-kDa蛋白质。这些结果表明放线共生放线杆菌Y 4和IDH 781对纤连蛋白具有高的结合亲和力,并且这种结合可能受40-kDa细菌细胞蛋白的调节。Y 4和IDH 781也显示出对IV型胶原的高亲和力,并且细菌细胞的非蛋白组分可能参与与IV型胶原的结合。远缘链球菌产生的细胞表面蛋白抗原(PAg)和葡萄糖基转移酶(GTF)是生物体的主要定植因子,并且这两个因子的抑制应该提供对龋齿的保护。在本研究中,我们构建了由PAg的富含丙氨酸的区域和GTF的葡聚糖结合结构域组成的融合蛋白(PAgA-GB),并在兔体内制备了针对该融合蛋白的抗体。抗PAgA-GB的抗体显著抑制蔗糖依赖性粘附,但不抑制蔗糖非依赖性粘附。这些结果表明,抗GTF-1的抗体可用于有效抑制S. sobrinus。然而,Ssobrinus的PAg可能不主要作为获得性表膜的受体发挥作用,并且其他细胞表面蛋白可能参与Ssobrinus的蔗糖非依赖性粘附。

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Effects of antibodies against a fusion protein consisting of parts of cell surface protein antigen and glucosyltransferase of Streptococcus sobrinus on cell adhesion of mutans streptococci.
  • DOI:
    10.1111/j.1399-302x.2007.00382.x
  • 发表时间:
    2007-12
  • 期刊:
  • 影响因子:
    0
  • 作者:
    T. Kawato;Y. Yamashita;T. Katono;A. Kimura;M. Maeno
  • 通讯作者:
    T. Kawato;Y. Yamashita;T. Katono;A. Kimura;M. Maeno
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KAWATO Takayuki其他文献

KAWATO Takayuki的其他文献

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{{ truncateString('KAWATO Takayuki', 18)}}的其他基金

The effects of periodontal disease on hepatic steatosis -an epidemiological and cell biological study-
牙周病对肝脂肪变性的影响——流行病学和细胞生物学研究——
  • 批准号:
    20K10298
  • 财政年份:
    2020
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Cell Biology and epidemiological studies of non-obese metabolic syndrome prevention focused on periodontal disease study
以牙周病研究为重点的非肥胖代谢综合征预防的细胞生物学和流行病学研究
  • 批准号:
    17K12022
  • 财政年份:
    2017
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The effects of cell interaction between osteoclast precursor cell and T cell on osteoclast differentiation.
破骨细胞前体细胞与T细胞之间的细胞相互作用对破骨细胞分化的影响。
  • 批准号:
    24792008
  • 财政年份:
    2012
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Continual Gram-negative bacterial challenge accelerates stroke onset SHRSP rats
持续的革兰氏阴性细菌攻击会加速 SHRSP 大鼠中风的发生
  • 批准号:
    21791832
  • 财政年份:
    2009
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)

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Development of endogenous functional recovery therapy after ischemic stroke targeting extracellular matrix protein
靶向细胞外基质蛋白的缺血性中风后内源性功能恢复疗法的进展
  • 批准号:
    21K20693
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    2021
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马拉色上清液上皮残渣细胞外基质蛋白的影响
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    20K10213
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    2020
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    $ 1.28万
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    Grant-in-Aid for Scientific Research (C)
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通过控制细胞外基质蛋白表达抑制主动脉瓣钙化的论证和机制阐明
  • 批准号:
    19K09089
  • 财政年份:
    2019
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  • 批准号:
    19K09511
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细胞外基质蛋白在胰岛素敏感性调节中的作用
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    18K16241
  • 财政年份:
    2018
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    Grant-in-Aid for Early-Career Scientists
Regulation of axon specification by a extracellular matrix protein, vitronectin in cerebellar granule cell precursors
小脑颗粒细胞前体细胞外基质蛋白玻连蛋白对轴突规范的调节
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    17K07105
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Serum extracellular matrix protein as a biomarker for acute exacerbation of interstitial pneumonia
血清细胞外基质蛋白作为间质性肺炎急性加重的生物标志物
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细胞外基质蛋白敲除及干细胞治疗建立盆腔器官脱垂模型小鼠
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    16K11147
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    2016
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Regulation of chondrocytes by extracellular matrix protein.
细胞外基质蛋白对软骨细胞的调节。
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    8776480
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A role of extracellular matrix protein versican in cell migration
细胞外基质蛋白多功能蛋白聚糖在细胞迁移中的作用
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    2013
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    Grant-in-Aid for Young Scientists (B)
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