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The effect of volatile sulfur compounds on the metabolism of alveolar bone.

挥发性硫化合物对牙槽骨代谢的影响。

基本信息

批准号：
17592191
负责人：
IMAI Toshio
金额：
$ 2.05万
依托单位：
The Nippon Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Oral malodorous compounds, hydrogen sulfide (H_2S) or methyl mercaptan, were periodontal pathogenic. However, the influence on the alveolar bone of the volatile sulfur compound has not been clarified. The aim of the study was to investigate the effect of H2_S on cell proliferation of human osteoblasts and on the differentiation and function of the human osteoclast precursors cell. After treatment of the NHOst cells with H2_S for 24 hours, [^3H]thymidine incorporation into the DNA is decreased significantly and H2_S dose-dependently. At a concentration of 100 ng/ml H2_S, [^3H]thymidine incorporation decreased 79% compared to the controls. To investigate the effect of H2_S on signal transduction pathways for controlling cell proliferation, we determined the Erk1/2 and p38 phosphorylation in the cells. The phosphorylation of Erk1/2 is increased by H2_S, dose-dependently. The phosphorylation was started at not longer than 10 min after starting H2_S incubation. Then, the phosphorylation sli … More ghtly decreased. Phosphorylation of p38 was also observed as well as Erkl/2.Receptor activator of nuclear factor κ B ligand (RANKL) plays a central role in the regulation of octeoblast differentiation and function. It was examined whether the H_2S induced the RANKL appearance from the osteoblast cell. The RANKL expression by the H_2S was not observed.We estimates that the effect of the hydrogen sulfide on the differentiation and of the function of osteoclast. Osteoclast formation was evaluated using tartrate-resistant acid phosphatase (TRAP) staining. The number of osteoclast formed was significantly lower in cells treated with H_2S compared to control cells. Osteoclast activity was evaluated using BD BioCoat^<TM> Osteologic^<TM> Bone cell culture system. The number of resorption pits in bone slices was significantly lower in cells treated with H_2S compared to control cells.These results suggests that volatile sulfur compound such as hydrogen sulfide may be a risk factor of the alveolus bone absorption. Less

口腔异味物质硫化氢（H_2S）或甲基硫醚是牙周致病物质。然而，挥发性硫化合物对牙槽骨的影响尚未明确。本研究旨在探讨硫化氢对人成骨细胞增殖及破骨细胞前体细胞分化和功能的影响。用H2_S处理NHOst细胞24小时后，[^3H]胸苷掺入DNA的量显著减少，并呈H2_S剂量依赖性。在100 ng/ml H2_S浓度下，与对照组相比，[^3H]胸苷掺入减少了79%。为了研究H2_S对控制细胞增殖的信号转导途径的影响，我们测定了细胞中Erk 1/2和p38磷酸化。H2_S可剂量依赖性地增加Erk 1/2的磷酸化。在开始H2_S孵育后不超过10分钟开始磷酸化。然后，磷酸化SLI ...更多信息下降。核因子κ B受体激活因子配体（RANKL）在成八细胞分化和功能调节中起重要作用。观察H_2S是否诱导成骨细胞RANKL的表达。H_2S对破骨细胞的分化及功能的影响可能与其对破骨细胞的增殖、分化及功能的影响有关。用抗酒石酸酸性磷酸酶（TRAP）染色评价破骨细胞形成。H_2S处理组破骨细胞形成数明显低于对照组。使用BD BioCoat ® <TM>Osteologic ® <TM>Bone细胞培养系统评价破骨细胞活性。H_2S处理组骨吸收陷窝数目明显少于对照组，提示H_2S等挥发性硫化物可能是引起骨吸收的一个危险因素。少