Effect of oral malodorous compound on the osteoclast differentiation and alveolar bone loss.

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The objective of this study was to elucidate the signal transduction mechanisms of hydrogen sulfide-induced osteoclast differentiation in primary cultued osteoclast precursor cells and RAW264 cells. Hydrogen sulfide at physiologic concentrations in mouth air induced the differentiation of mouse bone marrow-derived osteoclast precursor cells in the absence of RANKL. Moreover, hydrogen sulfide activated ERK1/2 phosphorylation in the osteoclast precursor cells. A low concentrations of NaHS induced PKC phosphorylation in RAW 264 cells. When pretreatment with PKC inhibitor(GF109203x), NaHS-induced the activation of PKC, c-Raf and ERK1/2 in RAW264 cells were diminished. These results suggested that hydrogen sulfide at physiologic concentrations stimulate the osteoclast differentiation through the PKC, cRaf, ERK1/2 pathway.