Importance and functional roles of methylmercaptan, one of the main causes of oral malodar, in progression of

甲硫醇（口腔恶臭的主要原因之一）在口腔恶臭进展中的重要性和功能作用

• 批准号: 17592189
• 负责人: YASUI Toshikazu
• 金额: $ 2.11万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592189/
• 关键词: methylmercapton; oral epithelial cell; necrosis; HMGB1; adult periodontal disease

Oral malodor is caused by volatile sulfur compounds (VSCs) including methylmercapton. Several studies showed that amount of methylmercapton are correlated with deeper pockets. In addition, resent studies show that periodontopathic bacteria produce the VSC. Therefore, it is proposed us that methylmercaptan plays a functional role (s) as pathogen factor for induction and progression of periodontal disease.Resent studies have investigated that many dead epithelial cells are detected in the area of deeper or bleeding pockets. However, it is not known the cytotoxic mechanisms of the cells, completely. And so, we examine a possibility that methylmercapton induces the cell death via using oral epithelial cell line KB cells and observed that the VSC exhibits cytotoxic effect on KB cells via necrosis manner.Periodontal disease is a chronic disease characterized by inflammation of gingival and resorption of alveolar bone in periodontal tissues. Resent studies indicated that virulence factors of … More pathogenic bacteria, such as lipopolysacchaaride (LPS), are able to stimulate inflammatory cytokines (IL-1, TNF-alpha, and IL-6), which are also inducers of bone resorption, and then, these cytokines closely contribute to progression of periodontal disease. It has been showed that hight mobility group protein 1 (HMGB1), an architectural chromatin-binding factor, is released from necrotic cells but not apoptotic cells and that HMGB1 is able to stimulate expression of IL-1 and TNF-alpha in monocyte and macrophage. Then, it has been suggested that HMGB1 is a DNA binding cytokine and also promote necrosis-induced inflammation. It is interesting to demonstrate that HMGB1 is secreted from necrotic KB cells destroyed by methylmercapton, and we examined whether the supernatant of the death cells is able to induce expression of TNF-alpha in mouse macrophage like cell line RAW264.7 cells. Then the supernatant stimulated the expression of TNF-alpha and the biological activity was abolished by treatment of anti-HMGB1 antibody.Therefore, it was suggested a possibility that methylmercapton induces the necrosis of oral epithelial cell and the necrosis is able to stimulate the expression of inflammation cytokines through the release of HMGB1 from the necrosis cells, in adult periodontal disease. Less

口腔异味是由包括甲硫醇在内的挥发性硫化物(VSCs)引起的。几项研究表明，甲硫醇的数量与更多的口袋相关。此外，最近的研究表明，牙周病原菌会产生VSC。因此，提出了甲硫醇作为致病因子在牙周病的发生和发展中的作用(S)。最近的研究发现，在牙周病的深部或出血区发现了许多死亡的上皮细胞。然而，目前尚不完全清楚该细胞的细胞毒性机制。因此，我们研究了甲硫醇通过口腔上皮细胞系KB细胞诱导细胞死亡的可能性，并观察到VSC通过坏死的方式对KB细胞产生细胞毒作用。牙周病是一种以牙周炎和牙周组织中的牙槽骨吸收为特征的慢性疾病。近年来的研究表明，…的毒力因子更多的病原菌，如脂多糖(LPS)，能够刺激炎性细胞因子(IL-1、TNF-α和IL-6)，这些细胞因子也是骨吸收的诱导者，然后，这些细胞因子与牙周病的进展密切相关。已有研究表明，高迁移率族蛋白1(HMGB1)是一种结构上的染色质结合因子，可从坏死细胞中释放，但不能从凋亡细胞中释放，并且HMGB1能刺激单核细胞和巨噬细胞表达IL-1和TNF-α。因此，有人认为HMGB1是一种DNA结合的细胞因子，也可以促进坏死诱导的炎症反应。有趣的是，HMGB1是由甲硫酮破坏的坏死性KB细胞分泌的，我们检测了死亡细胞的上清是否能够诱导小鼠巨噬细胞样细胞系RAW264.7细胞表达肿瘤坏死因子-α。培养上清液刺激肿瘤坏死因子-α的表达，经抗HMGB1抗体处理后，其生物学活性消失，提示甲硫醇可能通过释放HMGB1刺激炎症细胞因子的表达，从而导致口腔上皮细胞的坏死。较少

项目成果

Possible link between glycolysis and apoptosis induced by sodium fluoride

• DOI: 10.1177/154405910508401009
• 发表时间: 2005-10-01
• 期刊: JOURNAL OF DENTAL RESEARCH
• 影响因子: 7.6
• 作者: Otsuki, S;Morshed, SRM;Sakagami, H
• 通讯作者: Sakagami, H

Nitric oide-stimulated caspase-3-mediated apoptosis of mouse macrophage cell line RAW264.7 cells is disturbed by serum survival factor

一硝酸刺激 caspase-3 介导的小鼠巨噬细胞系 RAW264.7 细胞凋亡受血清存活因子干扰

• 发表时间: 2006
• 期刊: Meikai University Dental Journal 34
• 作者: Shinoda;H
• 通讯作者: H

Nitric oxide-stimulated caspase-3-mediated apoptosis of mouse macrophage cell line RAW264.7 cells is disturbed by serum survival factor

一氧化氮刺激的 caspase-3 介导的小鼠巨噬细胞系 RAW264.7 细胞凋亡受到血清存活因子的干扰

• 发表时间: 2005
• 期刊: Meikai University Dental Journal 34・2
• 作者: Tachibana;M.;Yoshida;A.et al.;Hiroyuki Shinoda
• 通讯作者: Hiroyuki Shinoda

A study on therelationship between occlusal condition and health philosophy or athlethic ability

咬合状况与健康理念或运动能力关系的研究

• 发表时间: 2007
• 期刊: The Journal of Meikai Dental Medicine Vol. 36-No. 1
• 作者: R.Ide;T.Hoshuyama;D.Wilson;K.Takahashi;T.Higashi;深井智子;Mayumi Suetsugu;Tomoko Fukai
• 通讯作者: Tomoko Fukai

Changes in fluoride sensitivity during in vitro senescence of normal human oral cells

正常人口腔细胞体外衰老过程中氟化物敏感性的变化

• 发表时间: 2005
• 期刊: Anticancer Res. 25
• 作者: Satoh;R
• 通讯作者: R