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Importance and functional rotes of methylmercaptan, one of the main causes of oral malodor, in induction or progression of adult periodontal disease

甲硫醇是口腔恶臭的主要原因之一，在成人牙周病的诱导或进展中的重要性和功能作用

基本信息

批准号：
15592213
负责人：
YASUI Toshikazu
金额：
$ 1.92万
依托单位：
Meikai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

Oral malodor is caused by volatile sulfur compounds (VSCs) including methylmercaptan. Several studies showed that amount of methylmercaptan are correlated with deeper pockets. In addition, resent studies show that periodontopathic bacteria produce the VSC. Therefore, it is proposed us that methylmercaptan plays a functional role (s) as pathogen factor for induction and progression of periodontal disease.Resent studies have investigated that many dead epithelial cells are detected in the area of deeper or bleeding pockets. However, it is not known the cytotoxic mechanisms of the cells, completely. And so, we examine a possibility that methylmercaptan induces the cell death via using oral epithelial cell line KB cells and observed that the VSC exhibits cytotoxic effect on KB cells via necrosis manner.Periodontal disease is a chronic disease characterized by inflammation of gingival and resorption of alveolar bone in periodontal tissues. Resent studies indicated that virulence factors of pathogenic bacteria, such as lipopolysaccharide (LPS), are able to stimulate inflammatory cytokines (IL-1, TNF-α, and IL-6), which are also inducers of bone resorption, and then, these cytokines closely contribute to progression of periodontal disease. Since these cytokines is abundantly produced by macrophage, the cellular apoptosis is requisite for the end of inflammation. Therefore, it is suggested that inhibition of the apoptosis of macrophage aggravates this disease, and we investigated effect of methylmercaptan on the cellular apoptosis vie using Raw 264.7 cells and observed that the VSC strongly inhibit nitric oxide-induced the apoptosis.

口腔异味是由挥发性硫化合物（VSCs）引起的，包括甲基硫醇。几项研究表明，甲基硫醇的数量与更深的口袋有关。此外，最近的研究表明牙周病细菌产生VSC。因此，我们认为甲基硫醇作为病原菌因子在牙周病的诱导和发展中起着功能性作用。最近的研究表明，在较深的或出血的口袋区域检测到许多死亡的上皮细胞。然而，细胞的细胞毒性机制尚不完全清楚。因此，我们研究了甲基硫醇通过口腔上皮细胞系KB细胞诱导细胞死亡的可能性，并观察到VSC通过坏死方式对KB细胞表现出细胞毒性作用。牙周病是一种以牙龈炎症和牙周组织牙槽骨吸收为特征的慢性疾病。最近的研究表明，致病菌的毒力因子如脂多糖（LPS）能够刺激炎症因子（IL-1、TNF-α和IL-6），这些因子也是骨吸收的诱导剂，这些细胞因子与牙周病的进展密切相关。由于巨噬细胞大量产生这些细胞因子，因此细胞凋亡是炎症结束所必需的。因此，我们认为抑制巨噬细胞的凋亡会加重这种疾病，我们用Raw 264.7细胞研究了甲基巯基醇对细胞凋亡的影响，发现VSC对一氧化氮诱导的细胞凋亡有较强的抑制作用。