Study on health effect of multiple mycotoxins and their control

多种霉菌毒素的健康影响及防治研究

基本信息

批准号：
17604008
负责人：
KONISHI Yoshiko
金额：
$ 2.3万
依托单位：
National Institute of Health Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Toxic secondary metabolites of fungi are called mycotoxins. As mycotoxins are natural toxins, occurrences are unavoidable problem in food safety. When these compounds are present in foods in sufficiently high level, they can have adverse health effects that range from acute to chronic. In chronic symptoms, aflatoxin is typical carcinogen. Among the derivatives of aflatoxins (B1,B2,G1,G2), aflatoxin B1(AFB1) has highest potential. Although AFB1 is contaminated with other mycotoxins in food, there is poor information about the effects of other mycotoxin on carcinogenic activity of AFB1. In this study, we established the assay for evaluation of the activity enhanced AFB1 carcinogenic activity by in vitro and in vivo. Among other three aflatoxins (B2,G1,G2) and two trichothecens mycotoxins (T-2 toxin and deoxynivalenol), in vitro experiment revealed that T-2 toxin enhanced AFB1 carcinogenic activity. In vivo experiment using F344 male rat also supported these in vitro results. In rats administrated with T-2 toxin, T cell population was decreased but B cell population was increased. NK cell activity was not changed. The activities of chytochrome P450 enzyme, especially CYP 3A4, 1A2 were increased. The activity related in the production of AFB1-DNA adduct was increased.In the study on control of soil bacteria for the production of mycotoxins, we found a useful bacterium for inhibition of mycotoxin that is luteoskyrin, production from Penicillium.islandicum. The bacteria were classified in Nocardia species by 16 s DNA sequence identification. These results indicate that the soil bacteria can inhibit production of mycotoxin without changing of ecological and mycological environment.

真菌的有毒次生代谢物称为霉菌毒素。由于霉菌毒素是天然毒素，因此食品安全中不可避免的问题。当这些化合物以足够高的食物中存在于食物中时，它们可能会产生从急性到慢性的不良健康影响。在慢性症状中，黄曲霉毒素是典型的致癌物。在黄曲霉毒素（B1，B2，G1，G2）的衍生物中，黄曲霉毒素B1（AFB1）具有最高的潜力。尽管AFB1在食物中被其他霉菌毒素污染，但关于其他霉菌毒素对AFB1致癌活性的影响的信息很差。在这项研究中，我们建立了通过体外和体内评估活性增强AFB1致癌活性的测定法。在其他三种黄曲霉毒素（B2，G1，G2）和两种三核酸蛋白霉菌毒素（T-2毒素和脱氧烯醇）中，体外实验表明T-2毒素增强了AFB1致癌活性。使用F344雄性大鼠的体内实验也支持这些体外结果。在用T-2毒素施用的大鼠中，T细胞群减少，但B细胞群增加。 NK细胞活性没有改变。 Chytoochrome P450酶的活性，尤其是CYP 3A4、1A2的活动。在对霉菌毒素产生的土壤细菌控制的研究中，增加了AFB1-DNA加合物的产生活性，我们发现了一种有用的细菌，可抑制脂肪毒素是黄体蛋白，由青霉素产生。通过16 s的DNA序列鉴定将细菌分类在诺卡氏菌中。这些结果表明，土壤细菌可以抑制霉菌毒素的产生，而不会改变生态和真菌环境。