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植物寄生線虫における性決定カスケードの解明

植物寄生线虫性别决定级联的阐明

基本信息

批准号：
22H02363
负责人：
新屋良治
金额：
$ 11.15万
依托单位：
Meiji University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2022
资助国家：
日本
起止时间：
2022-04-01 至 2025-03-31
项目状态：
未结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22H02363/
关键词：
ネコブセンチュウ性決定線虫糖 tra-1 植物寄生線虫

项目摘要

ネコブセンチュウ性分化に関わる遺伝子群を理解するために、トマトの根に感染後７日目の性未分化幼虫と、11日目の雌雄２-3期幼虫を根から解剖学的に取り出し、RNA-seq解析に供した。比較対象として、植物体感染前のネコブセンチュウ感染態2期幼虫も加えた。各３反復ずつ遺伝子発現プロファイルを取得し、主成分分析を実施した結果、４種類のサンプルそれぞれにおいて明確なグループが形成された。ネコブセンチュウにおいてその発現量に雌雄差が確認された遺伝子の中には、他種線虫において性決定に関与するものがあり、複数の転写因子も見出された。また、ネコブセンチュウは植物から摂取する糖を性決定のトリガーとして利用していることを示唆する結果を既に得ているが、モデル線虫C. elegansにおける主要グルコーストランスポーターのオルソログの発現がネコブセンチュウの雌雄で大きく異なることが明らかになった。次に、ネコブセンチュウtra-1遺伝子の機能解析を実施する準備に着手した。tra-1遺伝子はネコブセンチュウの近縁種において、性決定遺伝子としての機能を有することが明らかになっている。公開されているゲノム情報によるとネコブセンチュウは複数のtra-1オルソログを有しているが、主要tra-1と思われる遺伝子配列が他種線虫に比べ短く、ゲノム解析時の遺伝子予測にミスが生じている可能性が考えられた。そのため、今回ネコブセンチュウからcDNAを合成し、完全長配列の取得を試みた。その結果、実際にゲノム解析時の情報が誤りであることが明らかになった。現在では、このtra-1遺伝子の性決定における機能を調べるべく、ベンサミアナタバコにおけるVIGS系を用いた実験系の確立に取り組んでいる。現状、ネコブセンチュウがベンサミアナタバコにおよそ20%程度の寄生率を有することを確認できている。

The sexual differentiation subgroup understood that the undifferentiated larvae were sexually undifferentiated 7 days after infection, the 3rd stage larvae were male and female, the third stage larvae were anatomically dissected and RNA-seq analyzed. The infection rate is higher than that of infected plants and infected state 2 larvae. In each case, there are significant differences in the results of the principal component analysis, the results of the principal component analysis, and the results of the principal component analysis, the results of the principal component analysis and the results of the principal component analysis. We need to check the difference between male and female to make sure that there is a difference between male and female, that there is a difference between male and female, that there is a difference between male and female, that there is a difference between male and female, that there is a difference between male and female, that there is a difference between male and female, that there is a difference between male and female, that there is a difference between male and female, that there is a difference between male and female. The plants were told that the sugar was determined by sex. The results showed that the results of the disease were not only known but also known to the male and female students. The results showed that C. elegans was the main cause of the disease. In the second place, you will be able to parse the tra-1 computer and prepare to start the computer. The tra-1 information system is used to improve the performance of a variety of information systems and information systems. In public, we need to know that the number of copies of the tra-1 data is different, that the main tra-1 data sets are short, and that the data is not available when you analyze the data. This time, we will make sure that the cDNA is synthesized and the full length of the queue will be completed. The results of the test and the information on the analysis of the international information are important to ensure that you are fully informed. Now that the information is available, the information tra-1 will determine whether or not you will be able to use the information system to make sure that the VIGS system is located in the system. The parasitism rate is 20%. The parasitism rate is 20%. Please confirm that the parasitism rate is low.