Liver fibrosis investigation using iPSCs hepatic derived organ on chip

我们计划使用芯片上器官和肝细胞样细胞（hlc）、肝窦样细胞（LSECs）和星状细胞（hsc）三重共培养作为肝纤维化模型。纤维化将由棕榈酸触发，以进入从脂肪变性到肝纤维化的生理病理进展；TGF β是一种促纤维化因子，可直接诱导星状细胞活化。我们发表了一篇关于HLCs和LSECs共培养的论文。本文重点研究了健康条件下生物芯片内共培养的特点。我们建立了生物芯片是表达与分区相关的重要肝功能的相关模型。然后我们用健康条件下的三种培养扩展了我们的特征。三重培养似乎显示了我们现在正在分析的生物芯片中HSC的重要发展。在初步的三联培养实验中进行单细胞测序分析，提取生物芯片中hlc、LSECs、hsc的细胞亚群。我们还包括对照条件和暴露于TGF β的条件。

We are planning to use organ on chip and hepatocyte like cells (HLCs), liver sinusoidal like cells (LSECs) and stellate like cells (HSCs) triple coculture as a liver fibrosis model. The fibrosis will be triggered either by palmitic acid to access physio pathological progression from steatosis to liver fibrosis; either directly by the TGF beta that is a pro fibrotic factor inducing stellate cells activation.We published a paper dealing with HLCs and LSECs cocultures. The paper focuses on the characterisation of the coculture inside the biochips in the healthy condition. We established that the biochip is relevant model to express important liver function related to zonation. Then we extended our characterisation using the three cultures in healthy condition as well. The triple culture seems to show an important development of the HSC in biochip that we are now analysing. In parallel single cells sequencing analysis were performed on preliminary tri culture experiments to extract the cell subpopulation of HLCs, LSECs, HSCs in biochips. We also include control conditions and conditions exposed to TGF beta.