Liquid handling platform

除了蛋白质组学表达研究，蛋白质-蛋白质相互作用（PPI）和翻译后修饰（PTM）的分析是AG Beholds的一个特别研究重点，他在慕尼黑路德维希-马克西米利安大学（LMU）的系统神经科学集群（SyNergy）工作，研究通常被认为在神经退行性疾病中失调的分子机制。用于此类实验的质谱（MS）分析的样品制备是耗时的、劳动密集型的并且容易出错。因此，这里需要一个自动化的液体处理平台，以提高再现性，样品吞吐量和数据质量，并消除手动样品制备的主要瓶颈。所要求的仪器将由AG Besunds以分散的方式在LMU的SyNergy站点运行，并且是那里可用的MS单元的未来发展的不可或缺的补充。现场的其他工作组也可以访问该平台。在申请时，LMU Campus Großhadern没有MS兼容的液体处理平台。LMU生物医学中心蛋白质分析单元（ZfP）要求的可比较平台，适用于临床血清蛋白质组研究的范围非常窄，与蛋白质组PPI和PTM分析的更多可变要求不兼容。然而，这两个平台合在一起将涵盖一系列互补的方法。这里的目的是获得一个紧凑的仪器，结合灵活的应用可能性与高要求的再现在许多样品的强大的吞吐量。除了在肽的纯化和分级分离中的标准应用之外，可能的应用还应包括使用各种亲和基质和顺磁性、亲水相互作用色谱法对肽和蛋白质进行移液密集纯化。现场已经有了相应的空间和人员基础设施，这确保了顺利启动，并保证了参与应用的工作组的有效利用。

In addition to proteomic expression studies, the analysis of protein-protein interactions (PPIs) and post-translational modifications (PTMs) is a particular research focus of the AG Behrends, who is working at the Cluster for Systems Neuroscience (SyNergy) at Ludwig-Maximilians-Universität (LMU) Munich on molecular mechanisms that are often thought to be deregulated in neurodegenerative diseases. Sample preparations for mass spectrometry (MS) analyses of such experiments are time-consuming, labor-intensive and error-prone. Therefore, an automated liquid handling platform is requested here to improve reproducibility, sample throughput, and data quality, and to eliminate manual sample preparation as a major bottleneck. The requested instrumentation will be operated by the AG Behrends in a decentralized manner at the SyNergy site of LMU and is an indispensable complement for the future development of the MS units available there. Other working groups at the site such will also have access to this platform. At the time of application, no MS-compatible liquid handling platforms are available at the LMU Campus Großhadern. A comparable platform requested by the Protein Analysis Unit (ZfP) at the LMU Biomedical Center, with a very narrow scope of application to clinical serum proteome studies, is not compatible with the more variable requirements of proteomic PPI and PTM analyses. Taken together, however, the two platforms would cover a complementary spectrum of methods. The aim here is to acquire a compact instrumentation that combines flexible application possibilities with a high demand for reproduction in robust throughput of many samples. In addition to the standard applications in the desalting and fractionation of peptides, the possible applications should also include pipetting-intensive purifications of peptides and proteins using various affinity matrices and paramagnetic, hydrophilic interaction chromatography. A corresponding spatial and personnel infrastructure is already available at the site, which ensures a smooth start-up and guarantees efficient use for the working groups involved in the application.