Liquid handling platform

除了蛋白质组表达研究，蛋白质-蛋白质相互作用(PPI)和翻译后修饰(PTM)的分析是AG Behrends特别关注的研究重点，他在慕尼黑路德维希-马克西米利安大学(LMU)系统神经科学(SCHERGY)集群工作，研究通常被认为在神经退行性疾病中放松调控的分子机制。此类实验的质谱仪(MS)分析的样品制备耗时、费力且容易出错。因此，这里需要一个自动化的液体处理平台，以提高重现性、样品吞吐量和数据质量，并消除人工样品制备的主要瓶颈。所要求的仪器将由AG Behrends以分散的方式在LMU的协同地点运行，是那里现有MS单位未来发展不可或缺的补充。该网站的其他工作组也将使用这一平台。在申请时，洛桑州立大学格罗埃哈德恩校区没有MS兼容的液体处理平台。LMU生物医学中心蛋白质分析单位(ZFP)要求的一个类似平台，其应用范围非常狭窄，适用于临床血清蛋白质组研究，与蛋白质组PPI和PTM分析的更多可变要求不兼容。然而，这两个平台加在一起将涵盖一系列互补的方法。这里的目标是获得一种紧凑的仪器，它结合了灵活的应用可能性和对大量样品的稳健吞吐量的高再现要求。除了在多肽脱盐和分级方面的标准应用外，可能的应用还包括使用各种亲和基质和顺磁性、亲水性相互作用层析对多肽和蛋白质进行吸管强化纯化。场地已有相应的空间和人事基础设施，这确保顺利启动，并保证参与应用的工作组有效使用。

In addition to proteomic expression studies, the analysis of protein-protein interactions (PPIs) and post-translational modifications (PTMs) is a particular research focus of the AG Behrends, who is working at the Cluster for Systems Neuroscience (SyNergy) at Ludwig-Maximilians-Universität (LMU) Munich on molecular mechanisms that are often thought to be deregulated in neurodegenerative diseases. Sample preparations for mass spectrometry (MS) analyses of such experiments are time-consuming, labor-intensive and error-prone. Therefore, an automated liquid handling platform is requested here to improve reproducibility, sample throughput, and data quality, and to eliminate manual sample preparation as a major bottleneck. The requested instrumentation will be operated by the AG Behrends in a decentralized manner at the SyNergy site of LMU and is an indispensable complement for the future development of the MS units available there. Other working groups at the site such will also have access to this platform. At the time of application, no MS-compatible liquid handling platforms are available at the LMU Campus Großhadern. A comparable platform requested by the Protein Analysis Unit (ZfP) at the LMU Biomedical Center, with a very narrow scope of application to clinical serum proteome studies, is not compatible with the more variable requirements of proteomic PPI and PTM analyses. Taken together, however, the two platforms would cover a complementary spectrum of methods. The aim here is to acquire a compact instrumentation that combines flexible application possibilities with a high demand for reproduction in robust throughput of many samples. In addition to the standard applications in the desalting and fractionation of peptides, the possible applications should also include pipetting-intensive purifications of peptides and proteins using various affinity matrices and paramagnetic, hydrophilic interaction chromatography. A corresponding spatial and personnel infrastructure is already available at the site, which ensures a smooth start-up and guarantees efficient use for the working groups involved in the application.