C2: Data Science

项目摘要/摘要：核心2，数据科学 数据科学核心将促进和标准化所有研究项目的数据收集和分析 在这个U19项目中。特别是，我们将制定流程和系统，以收集、组织和 分析行为、成像、电生理学和神经操作数据。造福更广泛的人 神经科学界，我们将采用共享数据和元数据格式，并将我们的管道公开 可用，DataJoint作为科学数据管道的公共框架，而Neurodata没有 边框格式，以共享大量原始数据。该平台将促进对数据集的协作分析 项目内的研究人员，并使我们的分析具有可重复性和可扩展性。我们将使我们的 代码和数据在易于查找、开放访问的存储库中公开，例如Brain Initiative的分布式存储库 神经生理学数据集成和Github档案。我们使用这些通用数据标准将使 数据可互操作和可重复使用，从而确保我们的数据发布遵守公平的指导方针。 核心的第一个目标将是为神经生理学和 行为数据。我们已经有了用于收集虚拟现实行为数据的标准化数据管道 以及介镜成像和神经像素电生理记录的预处理。我们现在建议 通过三项新计划，将这项工作扩展到协作生成的所有数据。首先，我们将构建一个 共享平台，可通过模块化、用户友好的Web应用程序访问，以支持虚拟现实和 操作性条件反射任务。第二，我们将扩展我们的电生理和 钙成像数据支持几种最先进的分割算法。自动化 预处理、系统之间的数据传输以及人工管理步骤的标准化将使 分析更快、更容易，使神经生理学数据的处理更加有效和可重复性。 第三，我们将开发基础设施来支持行为期间的扰动，包括光遗传， 药理和物理操作。 核心的第二个目标将是记录系统，培训用户，并传播我们的计算 工具和工作流。这一努力将减轻研究人员的负担，通过促进 标准软件工具，增加标准化管道的采用，并促进其他人重新使用我们的数据。 为了促进这些管道的培训和使用，我们将开发基于Web的集成工具，以便 全球范围内对本地数据处理的访问和控制。这些工具的模块化性质将使它们 对更广泛的神经科学界有用并受欢迎。我们将提供持续的面对面培训 对所有研究人员和技术人员，包括与外部顾问的年度教程。这些方法加在一起， 用于自动化和标准化数据处理，当与更广泛的社区共享时，将改善 神经科学领域数据的重复性、可重用性和互操作性。

Project Summary/Abstract: Core 2, Data Science The Data Science Core will facilitate and standardize data collection and analysis for all research projects within this U19 program. In particular, we will develop processes and systems for collecting, organizing, and analyzing behavioral, imaging, electrophysiology, and neural manipulation data. To benefit the broader neuroscience community, we will adopt shared data and metadata formats and make our pipelines publicly available, with DataJoint as the common framework for scientific data pipelines and the Neurodata without Borders format to share large raw data. This platform will facilitate collaborative analysis of datasets by multiple researchers within the project, and make our analyses reproducible and extensible by others. We will make our code and data public in easy-to-find, open-access repositories, such as the BRAIN Initiative’s Distributed Archives for Neurophysiology Data Integration and Github. Our use of these common data standards will make the data interoperable and reusable, thus ensuring that our data publications adhere to FAIR guidelines. The core’s first aim will be to provide standardized computational pipelines for neurophysiological and behavioral data. We already have standardized data pipelines for collection of virtual-reality behavioral data and preprocessing of mesoscope imaging and Neuropixels electrophysiology recordings. We now propose to extend this effort to all data generated by the collaboration, via three new initiatives. First, we will construct a shared platform, accessed by modular, user-friendly web apps, to support virtual-reality and operant-conditioning tasks. Second, we will extend our preprocessing pipeline for electrophysiology and calcium imaging data to support several state-of-the-art segmentation algorithms. Automation of preprocessing, data transfer between systems, and standardization of manual curation steps will make analyses faster and easier, enabling more effective and reproducible processing of neurophysiological data. Third, we will develop infrastructure to support perturbations during behavior, including optogenetic, pharmacological, and physical manipulations. The core’s second aim will be to document the system, train users, and disseminate our computational tools and workflows. This effort will alleviate burdens on researchers, accelerate research by promoting standard software tools, increase adoption of standardized pipelines, and facilitate reuse of our data by others. To facilitate training and use of these pipelines, we will develop integrated web-based tools that allow world-wide access and control of local data processing. The modular nature of these tools will make them useful to and popular with the broader neuroscience community. We will provide continuous, in-person training to all researchers and technicians, including yearly tutorials with external consultants. Together, these methods for automating and standardizing data handling, when shared with the broader community, will improve the reproducibility, reusability, and interoperability of data across the field of neuroscience.