Compositional and structural analysis of DNA replication origins by locus-specific chromatin isolation (A29*)

点击翻译按钮获取中文摘要

Chromatin dynamics and the local chromatin organization critically contribute to the activity of DNA replication origins, but the precise chromatin and structural features like the exact nucleosome configuration, histone modification state or requirement of additional chromatin factors to allow efficient and timely replication initiation remain largely elusive. In this project, we will use locus-specific chromation isolation to target four distinct replication origins on yeast chromosome III that show major differences in their intrinsic replication properties regarding timing and firing efficiency. A combination of biochemical and functional assays is proposed to study whether the local chromatin structure determines the capacity of these regions to initiate DNA replication in vivo.