Tunneling Properties of Large Molecules

大分子的隧道特性

• 批准号: 05245102
• 负责人: IKAI Atsushi
• 金额: $ 83.33万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05245102/
• 关键词: tunneling property; large molecule; STM; AFM; organic molecules; biomolecules; STM; 脂肪酸; 脂肪族アルコール; ステアリン酸; ステアロイルアミド; ステアロイルアニリド; タンパク質; 生体膜; 液晶; 塩基

This study aimed at the elucidation of tunneling properties of bio/organic molecules and structures which are generally known to be electrically non-conductive.1 We were successful in imaging organic hydrocarbons with functional groups such as amine, carboxyl, anilide, alcohol, carbonyl, aryl, phenyl, cholestryl groups. The success opened a way to identify individual molecules by their functional groups in their mixed mono-layrs over the metal surface. Theoretical results of molecular orbital calculation of the intramolecular states of the electrons were applied to rationalize experimental identifications of such molecules.2 Scanning tunneling and atomic force microscopes were used in imaging proteins and nucleic acids. Regular arrays of adsorbed proteins on a metal surface were studied with an aim to cultivate useful methods for the creation of functional monolayr surfaces. The four bases that make up the genomic DNA were identified uder tunneling microscope with the help of calculated electron distribution.3 Methods of manipulation and engineering biomolecules and functional structures using atomic force microscope were developed. Stretching experiment of individual protein molecules and manipulation of the human genomic DNA in the chromosomes were successfully done.

本研究旨在阐明通常被认为是不导电的生物/有机分子和结构的隧穿特性我们成功地成像了具有官能团的有机碳氢化合物，如胺、羧基、苯胺、醇、羰基、芳基、苯基、胆固醇基。这一成功为通过金属表面混合单层中的官能团来识别单个分子开辟了一条道路。应用分子轨道计算电子分子内态的理论结果，使这类分子的实验鉴定合理化扫描隧道显微镜和原子力显微镜用于蛋白质和核酸成像。研究了金属表面上吸附蛋白质的规则阵列，目的是培养创建功能单层表面的有用方法。在隧道显微镜下，利用计算得到的电子分布，鉴定了组成基因组DNA的4个碱基发展了利用原子力显微镜对生物分子和功能结构进行操纵和工程的方法。成功地完成了单个蛋白质分子的拉伸实验和染色体中人类基因组DNA的操作。

项目成果

Katsumata,Ide: "Self-Assembly of 2-Thiocytosine Molecules Adsorbed on Au(111)Surface" Jpn. J. Appl. Phys.34. 3360-3362 (1995)

Katsumata，Ide：“吸附在 Au(111) 表面上的 2-硫胞嘧啶分子的自组装”Jpn。

猪飼 篤: "STM/AFMによるDNAの構造研究" 日本結晶学会誌. 35. 157-158 (1993)

Atsushi Ikai：“通过 STM/AFM 对 DNA 进行结构研究”日本晶体学会杂志 35. 157-158 (1993)。

KANETO,Keiichi: "Transport Mechanisms in evaports CO film evaluated by means of field effect" Jpn.J.Appl.Phys.35. 1802-1805 (1996)

KANETO,Keiichi：“通过场效应评估蒸发 CO 薄膜中的传输机制”Jpn.J.Appl.Phys.35。

MITSUI,Keita: "Mechanical unfolding of alpha-2-macroglobulin molecules with AFM" FEBS Letters. 385. 29-33 (1996)

MITSUI,Keita：“用 AFM 机械展开 α-2-巨球蛋白分子”FEBS 快报。

HARA,Masahiko: "Ordered nucleation of a self assembled monolayer on Au(111)studied by STM" Thin Solid Films. 273. 66-69 (1996)

HARA,Masahiko：“通过 STM 研究 Au(111) 上自组装单层的有序成核”固体薄膜。