Tunneling Properties of Large Molecules

这项研究的目的是阐明隧道特性的生物/有机分子和结构，通常被称为是不导电的。1我们成功地成像有机烃的官能团，如胺，羧基，苯胺，醇，羰基，芳基，苯基，胆甾醇基团。这一成功开辟了一种通过金属表面混合单层中的官能团来识别单个分子的方法。分子轨道计算的电子的分子内状态的理论结果被应用于合理化实验识别这样的分子。2扫描隧道和原子力显微镜被用于成像蛋白质和核酸。研究了蛋白质在金属表面的规则排列，旨在为功能性单层表面的制备提供有用的方法。在隧道显微镜下通过计算电子分布确定了组成基因组DNA的四个碱基。3发展了利用原子力显微镜操纵和工程化生物分子和功能结构的方法。成功地进行了蛋白质分子的拉伸实验和人类基因组DNA在染色体上的操作。

This study aimed at the elucidation of tunneling properties of bio/organic molecules and structures which are generally known to be electrically non-conductive.1 We were successful in imaging organic hydrocarbons with functional groups such as amine, carboxyl, anilide, alcohol, carbonyl, aryl, phenyl, cholestryl groups. The success opened a way to identify individual molecules by their functional groups in their mixed mono-layrs over the metal surface. Theoretical results of molecular orbital calculation of the intramolecular states of the electrons were applied to rationalize experimental identifications of such molecules.2 Scanning tunneling and atomic force microscopes were used in imaging proteins and nucleic acids. Regular arrays of adsorbed proteins on a metal surface were studied with an aim to cultivate useful methods for the creation of functional monolayr surfaces. The four bases that make up the genomic DNA were identified uder tunneling microscope with the help of calculated electron distribution.3 Methods of manipulation and engineering biomolecules and functional structures using atomic force microscope were developed. Stretching experiment of individual protein molecules and manipulation of the human genomic DNA in the chromosomes were successfully done.