Crosstalk between membrane organization and multi-sphere morphologies of complex membranes

膜组织与复杂膜的多球形态之间的串扰

• 批准号: 468578053
• 负责人: Professor Dr. Thomas Günther-Pomorski
• 金额: --
• 依托单位: Department of Biotechnology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/468578053?language=en
• 关键词: Crosstalk; between; membrane; organization; multi

Membrane lipid asymmetry is of paramount importance to the living cell in order to preserve its vital functions. Specific membrane proteins, termed lipid flippases, mediate the transport of lipids across cellular membranes and thereby enable the generation and maintenance of membrane lipid symmetry. Current concepts suggests that the dynamic process of lipid translocation may also help in controlling membrane domain organization and drive membrane vesicle formation. However, direct experimental evidence for these concepts is lacking. There is a need for the development of sophisticated tools that are suitable for studying these membrane transporters under well-defined conditions. In this project, we will establish a reconstitution system based on giant unilamellar vesicles. The central goal of our studies is a detailed functional analysis of lipid flippases in order to dissect their role in the regulation of membrane organization and morphology. We will explore membrane reorganization in the presence of functional lipid flippases under physiological conditions for cell-like lipid membrane compositions. We will measure the physical properties of membranes in response to the activity of these membrane transporters and analyze membrane-mediated cooperative effects at large and small length scales. We expect the powerful combination of biomimetic membrane systems and imaging methods to provide important new insights into the processes of into membrane reshaping processes by lipid flippases.

细胞膜脂质不对称对于维持活细胞的重要功能至关重要。特定的膜蛋白，称为脂质翻转酶，介导脂质跨细胞膜的运输，从而使膜脂对称的产生和维持。目前的概念表明，脂质转运的动态过程也可能有助于控制膜结构域的组织和驱动膜泡的形成。然而，这些概念缺乏直接的实验证据。需要开发适合在明确条件下研究这些膜转运蛋白的复杂工具。在本项目中，我们将建立一个基于巨型单层囊泡的重构系统。我们研究的中心目标是对脂质翻转酶进行详细的功能分析，以剖析它们在膜组织和形态调节中的作用。我们将探索在生理条件下存在功能性脂质翻转酶的膜重组对细胞样脂质膜组成的影响。我们将测量膜的物理性质，以响应这些膜转运蛋白的活性，并分析大、小长度尺度上膜介导的协同效应。我们期待仿生膜系统和成像方法的强大结合，为脂质翻转引起的膜重塑过程提供重要的新见解。