Function and dynamics of P4 ATPases in vesicular transport

P4 ATP酶在囊泡运输中的功能和动力学

• 批准号: 63935652
• 负责人: Professor Dr. Thomas Günther-Pomorski
• 金额: --
• 依托单位: Arbeitsgruppe Molekulare Zellbiochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/63935652?language=en
• 关键词: Function; dynamics; P4; ATPases; vesicular

Vesicle formation depends on an extensive network of interacting proteins that form large, dynamic modules to drive membrane budding. Recent studies have identified a distinct subfamily of P-type ATPases as putative phospholipid transporters that interact and cooperate with cytosolic coat proteins to drive vesicle formation at the trans-Golgi network, endosomal compartment and plasma membrane. The project aims to determine the mechanism by which these P-type ATPases function in vesicle biogenesis. Components of the P-type ATPase-coupled module will be reconstituted into giant liposomes to study their activities. The spatiotemporal localization of P4 ATPases and endocytic proteins will be analyzed in vivo by live-cell microscopy.

囊泡的形成依赖于一个广泛的相互作用的蛋白质网络，形成大的、动态的模块来驱动膜出芽。最近的研究已经确定了一个独特的p型atp酶亚家族，它被认为是磷脂转运蛋白，与细胞质外壳蛋白相互作用和合作，驱动反式高尔基网络、内体室和质膜上的囊泡形成。该项目旨在确定这些p型atp酶在囊泡生物发生中的作用机制。p型atp酶偶联模块的组分将被重组成巨大的脂质体来研究它们的活性。P4 atp酶和内吞蛋白的时空定位将通过活细胞显微镜在体内分析。

项目成果

A phospholipid uptake system in the model plant Arabidopsis thaliana

• DOI: 10.1038/ncomms8649
• 发表时间: 2015-07-01
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Poulsen, Lisbeth R.;Lopez-Marques, Rosa L.;Palmgren, Michael
• 通讯作者: Palmgren, Michael