Highly functional utilization of surplus renewable resources through microbial regio-specific hydroxylation

对丁香酚、异丁香酚、松香等可再生资源的微生物转化进行了研究。我们分离到一株丁香酚降解菌荧光假单胞菌E118，并试图建立从丁香酚和菜籽油中生产有用的化学物质阿魏酸的微生物工艺。我们还分离了一株异丁香酚降解菌，恶臭假单胞菌，并试图抛出的异丁香酚的微生物降解的轮廓光。恶臭假单胞菌P.putida 158的休眠细胞能快速地将异丁香酚通过香草醛转化为香草酸，转化率达98%。估计了异丁香酚双键直接裂解酶的存在。我们还分离到了一株具有较高羟基化活性的松香转化菌-卷枝毛霉。积累了大量的2a-羟基松香酸，具有高转化产率。本论文在丁香酚、异丁香酚和松香的微生物代谢方面取得了一些有意义的结果。基于这些有价值的信息和知识，我们打算建立新的生产工艺，从可再生资源，丁香酚，异丁香酚，菜籽油和松香有用的化合物。

The microbial conversion of renewable resources such as eugenol, isoeugenol, and rosin was studied. We isolated an eugenol-degrading bacterium, Pseudomonas fluorescens E118, and we attempted to establish the microbial production process of useful chemicals, ferulic acid, a useful antioxidant, from eugenol and clove oil. We also isolated an isoeugenol-degrading bacterium, Pseudomonas putida, and attempted to throw the light on the outline of the microbial degradation of isoeugenol. The resting cells of P.putida 158 rapidly converted isoeugenol into vanillic acid via vanillin with a conversion yield of 98%. The occurrence of the enzymes to catalyze the direct cleavage of double bond of isoeugenol was estimated. We also isolated rosin-converting microorganism, Mucor circinelloides which showed the high hydroxylation activity. The high amount of 2a-hydroxyabietic acid was accumulated with a high conversion yield. Some significant results regarding the microbial metabolisms of eugenol, isoeugenol and rosin were obtained through the present thesis. Based on the valuable information and knowledge, we intended to establish new production process of useful compounds from renewable resource, eugenol, isoeugenol, clove oil and rosin.