Highly functional utilization of surplus renewable resources through microbial regio-specific hydroxylation

通过微生物区域特异性羟基化对剩余可再生资源进行高度功能化利用

• 批准号: 14560062
• 负责人: NAGASAWA Toru
• 金额: $ 2.24万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560062/
• 关键词: rozin; eugenol; isoeugenol; renewable resource; bioconversion; hydroxylation; clove oil; ferulic acid; バニリン酸

The microbial conversion of renewable resources such as eugenol, isoeugenol, and rosin was studied. We isolated an eugenol-degrading bacterium, Pseudomonas fluorescens E118, and we attempted to establish the microbial production process of useful chemicals, ferulic acid, a useful antioxidant, from eugenol and clove oil. We also isolated an isoeugenol-degrading bacterium, Pseudomonas putida, and attempted to throw the light on the outline of the microbial degradation of isoeugenol. The resting cells of P.putida 158 rapidly converted isoeugenol into vanillic acid via vanillin with a conversion yield of 98%. The occurrence of the enzymes to catalyze the direct cleavage of double bond of isoeugenol was estimated. We also isolated rosin-converting microorganism, Mucor circinelloides which showed the high hydroxylation activity. The high amount of 2a-hydroxyabietic acid was accumulated with a high conversion yield. Some significant results regarding the microbial metabolisms of eugenol, isoeugenol and rosin were obtained through the present thesis. Based on the valuable information and knowledge, we intended to establish new production process of useful compounds from renewable resource, eugenol, isoeugenol, clove oil and rosin.

对丁香酚、异丁香酚、松香等可再生资源的微生物转化进行了研究。我们分离了一株丁香酚降解菌，荧光假单胞菌E118，并试图建立从丁香酚和丁香油中生产有用化学物质阿魏酸的微生物生产工艺。我们还分离了一株异丁香酚降解菌，恶臭假单胞菌，并试图揭示微生物降解异丁香酚的轮廓。恶臭假单胞菌158静息细胞通过香草醛快速转化异丁香酚为香草酸，转化率达98%。估计了催化异丁香酚双键直接断裂的酶的存在。我们还分离到了一株具有较高羟基化活性的松香转化微生物--环状毛霉。积累了大量的2a-羟基松香酸，转化率高。本论文在丁香酚、异丁香酚和松香的微生物代谢方面取得了一些有意义的结果。在此基础上，建立了以可再生资源丁香酚、异丁香酚、丁香油和松香为原料生产有用化合物的新工艺。

项目成果

K.Mitsukura et al.: "Microbial hydroxylation of rosin-related compounds"Journal of Bioscience and Bioengineering. (in press). (2003)

K.Mitsukura等人：“松香相关化合物的微生物羟基化”生物科学与生物工程杂志。

H.Furukawa et al.: "Conversion of isoeugenol into vanillic acid by Pseudomonas putida I15 cells exhibiting high isoeugenol-degrading activity"Journal of Bioscience and Bioengineering. 96(4). 401-403 (2003)

H.Furukawa 等人：“恶臭假单胞菌 I15 细胞表现出高异丁香酚降解活性，将异丁香酚转化为香草酸”《生物科学与生物工程杂志》。

Furukawa, S., Zenno, Y., Iwasawa, H.Morita, T.Yoshida, T.Nagasawa: "Ferulic Acid Production from Clove Oil by Pseudomonas fluorescens E118.H."J.Bioscience.Bioengineering.. 96(4). 404-405 (2003)

Furukawa, S.、Zenno, Y.、Iwasawa、H.Morita、T.Yoshida、T.Nagasawa：“荧光假单胞菌 E118.H 从丁香油生产阿魏酸”J.Bioscience.Bioengineering.. 96(4)

H.Furukawa et al.: "Ferulic acid production from clove oil by Pseudomonas fluorescens E118"Journal of Bioscience and Bioengineering. 96(4). 404-405 (2003)

H.Furukawa 等人：“荧光假单胞菌 E118 从丁香油生产阿魏酸”生物科学与生物工程杂志。

K.Mitsukura et al.: "Microbial hydroxylation frogin derivatives"Applied Microbiology and Biotechnology. 47(in press). (2004)

K.Mitsukura 等人：“微生物羟基化青蛙蛋白衍生物”应用微生物学和生物技术。