Library construction of decarboxylases catalyzing carbon dioxide fixation and their application for the production of various aromatic carboxylic acids

我们发现了几种芳香族或杂环羧酸脱羧酶催化Kolbe-Schmitt反应。我们对它们进行了鉴定，并对每个基因的高效表达和二氧化碳固定反应条件的优化进行了研究。我们构建了这些酶的文库，并提出了各种羧酸的生产工艺。这些酶由杂寡聚体和一个小分子亚基作为一个稳定因子参与，这对这些酶的进一步应用具有重要意义。

We have found several aromatic or heterocyclic carboxylic acid decarboxylases catalyze Kolbe-Schmitt reaction. We characterized them and the high expression of each gene and the optimization of reaction conditions of carbon dioxide fixation were studied. We constructed the library of these enzymes and proposed the production process of various carboxylic acids. These enzymes compose of hetero-oligomer and a small molecule subunit participates as a stable factor, which were very important for the further application of these enzymes.