Elucidation of adaptation mechanisms of lichens for dry condition used secondary metabolites from isolated mycobiont cultured in liquid medium

利用液体培养基中分离的真菌生物的次生代谢物阐明地衣对干燥条件的适应机制

• 批准号: 14560089
• 负责人: HAMADA Nobuo
• 金额: $ 1.98万
• 依托单位: Osaka City Institute of Public Health and Environmental Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560089/
• 关键词: Lichens; Isolation for Culture; Liquid culture; Secondary products; Graphis; 共生; 藻類; 地衣菌

It was thought that the secretion from the algal partner promotes to build up the symbiotic relationship with lichen mycobionts. In this project, isolation and identification of such chemical substances produced by algae was performed, using the technique, which cultures each partner separately. Mycobiont from one strain of Graphis, which produces different substances between isolated stage and symbiotic stage, was used for this project. We tried to survey substances from algae which have physiological activity to lichen mycobionts. Now we continue to try purification of this substance still. Next, lichen mycobiont and Trebouxia algal cells were grown in liquid medium with organic compounds. In BBM liquid medium hyphas penetrated the algal cells. In addition, penetration continued even after the culture was transferred to an agar medium. On the other hand, mycobiont colonies attached to the surface of the algal layer, when mycobionts and photobionts were next cultured on the agar medium.. However, neither mycobiont species penetrated the algal cell. In this study, we discussed the effect of drought and nutrient composition on the symbiosis between mycobionts and photobionts in lichens.

据认为，藻类伴侣的分泌物促进了与地衣菌的共生关系的建立。在该项目中，使用单独培养每个伙伴的技术，对藻类产生的这种化学物质进行了分离和鉴定。本项目使用了一株在分离阶段和共生阶段产生不同物质的葡萄属真菌。本研究试图从藻类中寻找对地衣菌有生理活性的物质。现在我们继续尝试提纯这种物质。接下来，地衣菌生体和Trebouxia藻细胞在含有有机化合物的液体培养基中生长。在BBM液体培养基中，菌丝穿透藻细胞。此外，即使在将培养物转移到琼脂培养基中后，渗透仍继续。另一方面，当接下来在琼脂培养基上培养菌生体和光生体时，菌生体菌落附着在藻类层的表面。然而，两种真菌都不能穿透藻细胞。本研究探讨了干旱和营养成分对地衣中真菌与光生菌共生关系的影响。

项目成果

Tanahashi T., Takenaka, Y., Nagakura, N., Hamada N: "Dibenzofurans from the cultured lichen mycobionts of Lecanora cineocarnera"Phytochemistry. 58. 1129-1134 (2002)

Tanahashi T.、Takenaka, Y.、Nagakura, N.、Hamada N：“来自 Lecanora cineocarnera 培养地衣真菌生物体的二苯并呋喃”植物化学。

T.Tanahashi, Y., Takenaka, N.Nagakura, N.Hamada: "Dibenzofurans from he cultured lichen mycobints of Lecanora cinereocarnera."Phytochemistry. 58. 1129-1134 (2002)

T.Tanahashi，Y.，Takenaka，N.Nagakura，N.Hamada：“来自他培养的 Lecanora cinereocarnera 地衣真菌的二苯并呋喃。”植物化学。

Tanahashi T., Takenaka, Y., Nagakura, N., Hamada N: "6H-Dibenzopyran-6-one derivatives from cultured lichen mycobionts of Graphis spp. and their biosynthetic origin"Phytochemistry. 62. 71-75 (2003)

Tanahashi T.、Takenaka, Y.、Nagakura, N.、Hamada N：“来自培养的地衣真菌菌种的 6H-二苯并吡喃-6-酮衍生物及其生物合成起源”植物化学。

Kawakatsu, M., Miyagawa, H., Hamada, N.: "Variations in the concentrations of chemical substances produced by cultured lichen mycobionts"Lichenology. 2・1. 11-15 (2003)

川胜M.、宫川H.、滨田N.：“培养地衣真菌产生的化学物质浓度的变化”地衣学2・1（2003）。

Tanahashi T., Takenaka, Y., Nagakura, N., Hamada N: "Production of anthraquinones by the cultured mycobionts of Xanliaoria polycarpone and X.elegans."Lichenology. 1・1. 7-10 (2002)

Tanahashi T.、Takenaka, Y.、Nagakura, N.、Hamada N：“Xanliaoria polycarpone 和 X.elegans 的培养菌体生产蒽醌”。地衣学 1・1。