Elucidation of adaptation mechanisms of lichens for dry condition used secondary metabolites from isolated mycobiont cultured in liquid medium

据认为，藻类伴侣的分泌促进了与地衣分枝体的共生关系。在这个项目中，使用单独培养每个伙伴的技术，对藻类产生的这些化学物质进行了分离和鉴定。本研究选用一株葡萄球菌的真菌，该菌株在分离阶段和共生阶段产生不同的物质。我们试图从藻类到地衣分枝生物中调查具有生理活性的物质。现在我们还在继续提纯这种物质。然后，在含有机化合物的液体培养基中培养地衣菌体和海藻细胞。在BBM液体培养基中，菌丝渗透到藻类细胞中。此外，即使将培养物转移到琼脂培养基上，渗透也在继续。另一方面，真菌菌落附着在藻层表面，当真菌菌落和光菌落在琼脂培养基上培养时。然而，这两种真菌都没有渗透到藻类细胞中。本研究探讨了干旱和营养成分对地衣中分枝生物和光生物共生的影响。

It was thought that the secretion from the algal partner promotes to build up the symbiotic relationship with lichen mycobionts. In this project, isolation and identification of such chemical substances produced by algae was performed, using the technique, which cultures each partner separately. Mycobiont from one strain of Graphis, which produces different substances between isolated stage and symbiotic stage, was used for this project. We tried to survey substances from algae which have physiological activity to lichen mycobionts. Now we continue to try purification of this substance still. Next, lichen mycobiont and Trebouxia algal cells were grown in liquid medium with organic compounds. In BBM liquid medium hyphas penetrated the algal cells. In addition, penetration continued even after the culture was transferred to an agar medium. On the other hand, mycobiont colonies attached to the surface of the algal layer, when mycobionts and photobionts were next cultured on the agar medium.. However, neither mycobiont species penetrated the algal cell. In this study, we discussed the effect of drought and nutrient composition on the symbiosis between mycobionts and photobionts in lichens.