Screening new substances produced by lichen mycobionts cultured under osmotic condition

筛选渗透条件下培养的地衣真菌产生的新物质

• 批准号: 09660122
• 负责人: HAMADA Nobuo
• 金额: $ 2.18万
• 依托单位: Osaka City Institute of Public Health And Environmental Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660122/
• 关键词: Lichens; Isolation for Culture; Osmotic Condition; Secondary products; 二次代謝産物; 高浸透圧培養; ナフトキノン

We collected many samples of lichens included to Lecanora, Graphis and others in the southern islands of Japan. for elucidating the symbiotic mechanism on lichens. The isolated mycobionts from collected samples were cultured on the media added 10% sucrose, and the secondary products in mycobiont colonies were examined. Those products were very often new and unique, and already identified during 2 years were the following : Naphthaquinone derivatives were discovered in mycobionts of Lecanora nipponica and Graphis sp., collected in Iriomote Island, dibenzofurans in Stereocaulon japonica and graphislactones in Pyrenula japonica, respectively. Some other products are still on the way for identification, and will be identified within a couple of months.Noteworthly, some products in mycobionts were also found in free-living fungi (for example Alternaria). The same products distributed worldwide, Graphenone found in Japanese Graphis app. were also found in Graphis ap. from Argentina.

我们收集了许多地衣样本，包括日本南部岛屿的Lecanora， Graphis和其他地衣样本。阐明地衣的共生机制。将收集的样品分离出的分枝菌在添加10%蔗糖的培养基上培养，并检测分枝菌菌落的二次产物。这些产物通常是新的和独特的，在2年内已经鉴定出的有：在伊里奥莫特岛收集的日本Lecanora nipponica和Graphis sp.分枝菌中分别发现萘醌衍生物，在日本Stereocaulon japonica中分别发现二苯并呋喃和在日本Pyrenula japonica中分别发现石墨烯内酯。其他一些产品仍在进行鉴定，并将在几个月内进行鉴定。值得注意的是，在自由生活的真菌（如Alternaria）中也发现了分枝杆菌中的一些产物。在日本Graphis应用程序中发现的石墨烯酮，在阿根廷Graphis应用程序中也发现了相同的产品。

项目成果

M.Miyagawa: "Hypostepsilic acid from a cultured lichem mycobionts of Stereocaulou Japanicum" Phytochemistry. 46(7). 1289-1291 (1997)

M.Miyakawa：“来自 Stereocaulou Japanicum 培养的地衣真菌生物体的 Hyposteppsilic 酸”植物化学。

H.Miyagawa: "Hypostrepsilic acid from a cultured lichen mycobionts of Stereocanlon Japonicuin" Phytochcmistry. 46(7). 1289-1291 (1997)

H.Miyakawa：“来自培养的 Stereocanlon Japonicuin 地衣真菌生物体的下螺旋酸”植物化学。

Hiromi Miyawaki, Masafumi Kawaguchi, Nobuo Hamada and Isao Yoshimura: "A correlation between B-orcinol para depside atranorin and orcinol depside lecanoric acid in Parmotrema tinctorum." Sauteria. 9. 237-246 (1998)

Hiromi Miyawaki、Masafumi Kawaguchi、Nobuo Hamada 和 Isao Yoshimura：“Parmotrematinctorum 中 B-苔黑酚对缩酚酸阿曲诺林和苔黑酚缩酚酸 lecanoric 酸之间的相关性。”

N.Hamada: "Induction of secondary products in isolated mycobionts from north American lichens" Symbiosis. 23. 219-224 (1997)

N.Hamada：“从北美地衣中分离出的真菌生物中诱导次级产物”共生。

N.Hamada et al.: "Induction of secondary products in Isolated mycobionts from North American licheus" Symbiosis. 23. 219-224 (1997)

N.Hamada 等人：“从北美地衣中分离出的真菌生物中诱导次级产物”共生。