Screening new substances produced by lichen mycobionts cultured under osmotic condition

我们收集了许多地衣样品，包括日本南部岛屿的Lecanora，Graphis和其他地衣。用于阐明地衣上的共生机制。从采集的样品中分离的真菌在添加10%蔗糖的培养基上培养，并检查真菌菌落中的次级产物。这些产品往往是新的和独特的，并已确定在2年内如下：萘醌衍生物被发现在真菌的Lecanora nipponica和Graphis sp.，在西表岛采集的二苯并呋喃类化合物，在日本硬枝藻（Stereocaulon japonica）采集的二苯并呋喃类化合物和在日本核壳藻（Pyrenula japonica）采集的石墨内酯类化合物。其他一些产物仍在鉴定过程中，将在几个月内鉴定出来。值得注意的是，在真菌中也发现了一些产物（如链格孢属）。在全球范围内销售的相同产品，在日本Graphis应用程序中发现的石墨烯也在Graphis应用程序中发现。来自阿根廷。

We collected many samples of lichens included to Lecanora, Graphis and others in the southern islands of Japan. for elucidating the symbiotic mechanism on lichens. The isolated mycobionts from collected samples were cultured on the media added 10% sucrose, and the secondary products in mycobiont colonies were examined. Those products were very often new and unique, and already identified during 2 years were the following : Naphthaquinone derivatives were discovered in mycobionts of Lecanora nipponica and Graphis sp., collected in Iriomote Island, dibenzofurans in Stereocaulon japonica and graphislactones in Pyrenula japonica, respectively. Some other products are still on the way for identification, and will be identified within a couple of months.Noteworthly, some products in mycobionts were also found in free-living fungi (for example Alternaria). The same products distributed worldwide, Graphenone found in Japanese Graphis app. were also found in Graphis ap. from Argentina.