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Studies on the regulation, by proteins and amino acids of pathogenisis of hepatitis

蛋白质和氨基酸对肝炎发病机制的调控研究

基本信息

批准号：
14560096
负责人：
SUGIYAMA Kimio
金额：
$ 2.24万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560096/
关键词：
protein amino acid hepatitis lipopolysaccharide D-galcatosamine rat LPS アポトーシス TNF-α NO 大豆タンパク質含硫アミノ酸

项目摘要

This study was undertaken to investigate the effects of dietary proteins and amino on LPS/Ga1N-induced hepatitis and to clarify the mechanism underlying the effects of proteins and amino acids in rats.(1)When rats were fed diets containing one of milk casein, whole egg protein, soybean protein, and wheat gluten as protein sources and then hepatitis was evoked by the injection of LPS/Ga1N, hepatitis was most potently suppressed by feeding of soybean protein. It was suggested that amino acid composition of soybean protein, in addition to impurities of the protein, might be participated in the effect of soybean protein.(2)Feeding of rats with low casein diet, as compared with standard or high casein diet, suppressed LPS/Ga1N-induced hepatitis and supplementation of the low casein diet with cysteine at a low level potentiated the supprerssive effect of low cvasein diet. These results indicate that low protein diet with a high nutritive value leads to marked suppression of LPS/Ga1N-induced hepatitis.(3)When 20 kinds of amino acids were added singly to the diet, LPS/Ga1N-induced hepatitis was markedly suppressed by cetrtain kinds of amino acids (Gly, val, lie, Lys, His).(4)The mechanism by which soybean protein and several amino acids suppressed LPS/Ga1N-induced hepatitis was investigated in terms of plasma cytokine and nitric oxide concentration. The results suggest that, in most cases, dietary protein and supplemented amino acids elicit their effect through inhibition of LPS-induced promotion of nitric oxide production rather than through inhibition of LPS-induced promotion of tumor necrosis factor alpha.(5)The results of this study show that liver function, especially the sensitivity of the liver to hepatitis, can be largely infuemced by dietary proteins and amino acids, suggesting that certain types of dietary proteins and amino acids might be useful to regulate the pathogenesis of hepatitis.

本研究旨在探讨膳食蛋白质和氨基酸对LPS/ ga1n诱导大鼠肝炎的影响，并阐明蛋白质和氨基酸对大鼠肝炎的影响机制。(1)饲喂乳酪蛋白、全蛋蛋白、大豆蛋白和麦麸蛋白各一种作为蛋白源的饲料，注射LPS/Ga1N诱发大鼠肝炎，其中大豆蛋白对肝炎的抑制作用最强。结果表明，大豆蛋白的作用除杂质外，还可能与氨基酸组成有关。(2)与标准或高酪蛋白饲粮相比，低酪蛋白饲粮对LPS/ ga1n诱导的肝炎有抑制作用，低酪蛋白饲粮中添加低水平半胱氨酸可增强低酪蛋白饲粮的抑制作用。由此可见，高营养价值的低蛋白饲粮对LPS/ ga1n诱导的肝炎有明显的抑制作用。(3)饲粮中单独添加20种氨基酸时，某些氨基酸（Gly、val、lie、Lys、His）对LPS/ ga1n诱导肝炎有明显抑制作用。(4)从血浆细胞因子和一氧化氮浓度方面探讨大豆蛋白和几种氨基酸抑制LPS/ ga1n诱导肝炎的机制。结果表明，在大多数情况下，膳食蛋白质和补充氨基酸通过抑制lps诱导的促进一氧化氮生成而不是通过抑制lps诱导的促进肿瘤坏死因子α来发挥其作用。(5)本研究结果表明，肝脏功能，特别是肝脏对肝炎的敏感性在很大程度上受到膳食蛋白质和氨基酸的影响，提示某些类型的膳食蛋白质和氨基酸可能有助于调节肝炎的发病机制。