Studies of molecular Nutrition on Retinoid absorption and metabolism

分子营养对类维生素A吸收和代谢的研究

• 批准号: 14570066
• 负责人: TAKASE Sachiko
• 金额: $ 2.11万
• 依托单位: Siebold University of Nagasaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570066/
• 关键词: CRBPII; PPARS; coactivator (p300); β-carotene dioxygenase; chick duodenum; Hydrocorticoid; rat jejunum; thyroid hormone (T3); CRBP II; 発達過程; レチノール吸収; PPARα; PPARδ; coactivator; CBP; p300; rat intestine

1. Our investigations revealed that gene expression levels of cellular retinol-binding protein type II (CRBPII) is modulated through postnatal development of PPAR subtypes and endogenous transcriptional activator p300. This p300 coactivator is interacted with PPARa or PPAR6 in the presence of their specific ligands such as unsaturated fatty acids. During the weaning period (17day -25day ages) the PPARa/b ratio increased and CRBPII mRNA strongly expressed.2. Developmental expression pattern, of frcarotene cleavage enzyme (BCCE) activities are increased during the postnal development of small intestine of rats as well as chicks. The BCCE activity in rat jejunum was elevated during the weaning period. In chick case, duodenal BCCE activity was induced after their birth with its peak activity at 5 days after their hatching. The BCCE activity in rat jejunum was elevated by hormones of hydrocortisone (glucocorticoid) as well as thyroid hormone T3. The weaning diets containing high or low diet … More ary fat affected its activity showing that the high fat-feeding induced low BCCE activity and the low fat-feeding induced high BCCE activity in weaning rats. This results may explain why mother milk feeding during suckring period induce lower activity of BCCE in rat jejunum and why higher activity after weaning during rat development.3. After hatching, chick duodenum expressed highly BCCE mRNA, which levels increased developmentally and its peak expression occurred at 5 days after thir birth. The administration of hydrocortisone to chick embryo on day 14 and 17 of incubation, developmental profile of BCCE mRNA level changed to early its induction. The results suggested that endogeneous hormone level might involed to in the developmental induction of intestinal BCCE gene expression.4. It is clalified that dietary high fat induced CRBPII gene expression are accompanied with increases in intestinal retinol absorption and liver uptake of retinol delivered from intestine as well as chromicrone synthesis by using a indicator of microsomal transfer protein mRNA expression. Less

1.我们的研究表明，细胞视黄醇结合蛋白II型（CRBPII）的基因表达水平是通过出生后发育的过氧化物酶体增殖物激活物亚型和内源性转录激活因子p300调制。这种p300共活化剂在其特异性配体如不饱和脂肪酸存在下与PPARa或PPAR 6相互作用。在断奶期（17日龄-25日龄），PPARa/B比值升高，CRBP Ⅱ mRNA表达增强.在大鼠和鸡小肠发育后期，β-胡萝卜素裂解酶（BCCE）活性的发育性表达模式都是增加的。断乳期大鼠空肠中BCCE活性升高。雏鸡十二指肠BCCE活性在出生后即被诱导，出壳后5d达到高峰。氢化可的松（糖皮质激素）和甲状腺激素T3可使大鼠空肠BCCE活性升高。高、低日粮组成的断奶日粮 ...更多信息 脂肪影响其活性，高脂喂养导致断乳大鼠BCCE活性降低，低脂喂养导致断乳大鼠BCCE活性升高。这一结果可能解释了哺乳期母乳喂养导致大鼠空肠BCCE活性降低以及断奶后大鼠空肠BCCE活性升高的原因.出壳后，鸡十二指肠高表达BCCEmRNA，其表达量随发育而增加，生后5天表达量最高。孵育第14和17天的鸡胚给予氢化可的松后，BCCE mRNA水平的发育曲线向其诱导早期转变。提示内源激素水平可能参与了小肠BCCE基因表达的发育诱导.通过使用微粒体转移蛋白mRNA表达的指示剂，阐明了饮食高脂肪诱导的CRBPII基因表达伴随着肠视黄醇吸收和肝脏摄取从肠递送的视黄醇以及铬微粒体合成的增加。少

项目成果

高瀬幸子, 四童子好廣, 山本武: "小腸におけるβカロテン代謝調節のメカニズム究明"平成13年度シーボルト大学共同教育研究報告書. 227-230 (2003)

Sachiko Takase、Yoshihiro Shidoji、Takeshi Yamamoto：“小肠中 β-胡萝卜素代谢调节机制的研究”2001 Siebold 大学联合教育研究报告 227-230 (2003)。

合田敏尚, 駿河和仁, 高瀬幸子: "ビタミンAの腸管吸収"細胞. 34(3). 88-91 (2002)

Toshihisa Goda、Kazuhito Suruga、Sachiko Takase：“维生素 A 的肠道吸收”细胞 34(3)。

高瀬幸子 他(分担執筆): "プロビタミンAとしての発見と研究,第2章カロテノイド"(日本ビタミン学会編)ビタミン:研究のブレークスルー(学進出版). 21-24 (2002)

Sachiko Takase 等人（合著者）：“维生素原 A 的发现和研究，第 2 章类胡萝卜素”（日本维生素协会编辑）维生素：研究突破（Gakushin Publishing）21-24（2002 年）。

Mochizuki K, Suruga K, Sakaguchi N, Takase S, Goda T: "Major intestinal coactivator p300 strongly activates peroxisome proliferator-activated receptor in intestinal cell line, Caco-2."GENE. 291. 271-277 (2002)

Mochizuki K、Suruga K、Sakaguchi N、Takase S、Goda T：“主要肠道共激活剂 p300 强烈激活肠道细胞系 Caco-2 中的过氧化物酶体增殖物激活受体。”GENE。

高瀬幸子 他(分担執筆): "ビタミン(日本ビタミン学会編)"ビタミン:研究のブレークスルー,学進出版. 21-24 (2002)

Sachiko Takase 等人（合著者）：“维生素（日本维生素协会编辑）”维生素：研究突破，Gakushin Publishing 21-24（2002）。