Molecular and pharmacological investigation of ATP-sensitive K^+ channels in human detrusor

点击翻译按钮获取中文摘要

Recent molecular cloning studies have revealed that ATP-sensitive K^+ channels (K_<ATP> channels) are heteromeric complexes composed of at least two subunits, inwardly rectifying K^+ channels (Kir6.x) and sulphonylurea receptors (SURs). From functional expression experiments, pharmacological and electrophysiological studies have indicated that SUR1/Kir6.2 represents the pancreatic β-cell K_<ATP> channel, that SUR2A/Kir6.2 is thought to represent the cardiac K_<ATP> channel, whereas SUR2B/Kir6.1 may represent the vascular smooth muscle-type K_<ATP> channel.In the present experiments, we have investigated molecular and pharmacological properties of K_<ATP> channels in human urinary bladder smooth muscle. We have revealed that the pore region of Kir6.x in human urinary bladder-type K_<ATP> channels may mainly consist of Kir6.2 due to the results of Western blotting techniques. Furthermore, the subunits of SURs in human urinary bladder-type K_<ATP> channels are likely to be mixture of SUR1 and SUR2B.