Molecular and pharmacological studies of human vascular-type ATP-sensitive K+ channels regarding the channel kinetics

人血管型 ATP 敏感 K 通道有关通道动力学的分子和药理学研究

• 批准号: 16390067
• 负责人: TERAMOTO Noriyoshi
• 金额: $ 9.22万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390067/
• 关键词: ATP; Ion channels; Vascular smooth muscle; Receptor; ヒト血管平滑筋細胞; ATP感受性カリウムチャネル; 国際研究者交流

Potassium channels which are inhibited by intracellular ATP (ATP_i) were first identified in ventricular myocytes, referring to as ATP-sensitive K^+ channels (i.e. K_<ATP> channels). Subsequently, K^+ channels with similar characteristics have been demonstrated in many other tissues (pancreatic β-cells, skeletal muscle, central neurones, smooth muscle). Approximately one decade ago, K_<ATP> channels were cloned and were found to be composed of at least two subunits : an inwardly-rectifying K^+ channel six family (Kir6.x) which forms the ion conducting pore and a modulatory sulphonylurea receptor (SUR) that accounts for several pharmacological properties. Various types of native K_<ATP> channels have been identified in a number of visceral and vascular smooth muscles in single-channel recordings. However, little attention has been paid to the molecular properties of the subunits in K_<ATP> channels and it is important to determine the relative expression of K_<ATP> channel components which give rise to native K_<ATP> channels in smooth muscle. The aim of this study was to investigate molecular properties of K_<ATP> channels with a main interest in the molecular basis of native K_<ATP> channels and to discuss their possible linkage with physiological functions in smooth muscle. We have prepared the original article which will be submitted to some journal soon.

被细胞内三磷酸腺苷(ATPI)抑制的钾通道最早是在心肌细胞中发现的，称为ATP敏感性钾通道(K&lt；ATP&gt；通道)。随后，在许多其他组织(胰腺β细胞、骨骼肌、中枢神经元、平滑肌)中也发现了具有相似特性的K~+通道。大约10年前，K_&lt；ATP&gt；通道被克隆，并被发现至少由两个亚基组成：形成离子传导孔的内向整流性K~(++)通道6家族(Kir6.x)和与多种药理特性有关的调节性磺脲受体(SUR)。在单通道记录中，已在许多内脏和血管平滑肌中发现了不同类型的天然K_lt；ATP&gt；通道。然而，对K_lt；ATP&gt；通道亚基的分子特性研究较少，因此确定K_lt；ATP&gt；通道组分在平滑肌中的相对表达具有重要意义。本研究的目的是研究K&lt；ATP&gt；通道的分子特性，主要关注天然K&lt；ATP&gt；通道的分子基础，并探讨它们与平滑肌生理功能的可能联系。我们已经准备好了原文，很快就会投稿到一些期刊上。

项目成果

Mefenamic acid as a novel activator of L-type voltage-dependent Ca^<2+> channels in smooth muscle from pig proximal urethra.

甲芬那酸作为猪近端尿道平滑肌中 L 型电压依赖性 Ca^2 通道的新型激活剂。

• 发表时间: 2005
• 期刊: British Journal of Pharmacology 144
• 作者: Teramoto;N. et al.
• 通讯作者: N. et al.

The effects of flavoxate hydrochloride on voltage-dependent L-type Ca2+ currents in human urinary bladder.

盐酸黄酮酯对人膀胱电压依赖性 L 型 Ca2 电流的影响。

• 发表时间: 2005
• 期刊: The British Journal of Pharmacology 46
• 作者: Tomoda T;et al.
• 通讯作者: et al.

Effects of U-37883A on intracellular Ca2+ -activated large-conductance K+ channels in pig proximal urethral myocytes.

U-37883A 对猪近端尿道肌细胞内 Ca2 激活的大电导 K 通道的影响。

• DOI: 10.1016/j.ejphar.2004.10.025
• 发表时间: 2004
• 期刊: European journal of pharmacology
• 影响因子: 5
• 作者: N. Teramoto;M. Aishima;Hai‐Lei Zhu;T. Tomoda;T. Yunoki;F. Takahashi;A. Brading;Y. Ito
• 通讯作者: Y. Ito

An analysis of inhibitory junction potentials in the guinea-pig proximal colon

• DOI: 10.1113/jphysiol.2004.065052
• 发表时间: 2004-08-01
• 期刊: JOURNAL OF PHYSIOLOGY-LONDON
• 影响因子: 5.5
• 作者: Hirst, GDS;Bywater, RAR;Edwards, FR
• 通讯作者: Edwards, FR

Modulation of voltage‐dependent Ba2+ currents in the guinea‐pig gastric antrum by cyclic nucleotide‐dependent pathways

• DOI: 10.1038/sj.bjp.0706295
• 发表时间: 2005-09
• 期刊: British Journal of Pharmacology
• 影响因子: 7.3
• 作者: Hai‐Lei Zhu;G. Hirst;Y. Ito;N. Teramoto